本文選題：臍帶間充質(zhì)干細(xì)胞　切入點(diǎn)：乳腺上皮細(xì)胞　出處：《畜牧獸醫(yī)學(xué)報(bào)》2017年05期 　論文類型：期刊論文

【摘要】：旨在探究奶牛乳腺上皮細(xì)胞(BMECs)與臍帶間充質(zhì)干細(xì)胞(UC-MSCs)共培養(yǎng)對(duì)(BMECs)乳脂合成及關(guān)鍵基因表達(dá)的影響。試驗(yàn)共分為8組:共培養(yǎng)組為UC-MSCs和BMECs共培養(yǎng)條件下的不處理組、IGF-1R抑制劑AG1024處理組、Janus激酶和轉(zhuǎn)錄活化因子(JAK/STAT)信號(hào)通路信號(hào)阻斷劑AG490處理組及AG1024+AG490處理組,對(duì)照組為BMECs單培養(yǎng)條件下的不處理組、IGF-1R抑制劑AG1024組、Janus激酶和轉(zhuǎn)錄活化因子(JAK/STAT)信號(hào)通路信號(hào)阻斷劑AG490組及AG1024+AG490處理組。檢測(cè)各組上清IGF-1、甘油三酯(TG)含量變化;RT-qPCR檢測(cè)乙酰輔酶A羧化酶(ACACA),脂肪酸合成酶(FASN)和固醇調(diào)節(jié)元件結(jié)合蛋白(Sterol regulatory element binding proteins,SREBP1)基因的相對(duì)表達(dá)豐度。結(jié)果表明,共培養(yǎng)組IGF-1、TG含量均顯著高于對(duì)照組(P0.05);AG1024處理對(duì)IGF-1具有極顯著抑制效果(P0.01),顯著降低TG含量及ACACA、FASN、SREBP1mRNA相對(duì)表達(dá)豐度(P0.05);AG490處理對(duì)ACACA、FASN、SREBP1mRNA的表達(dá)無顯著影響(P0.05);AG1024和AG490共同處理較AG1024單獨(dú)處理各項(xiàng)指標(biāo)表現(xiàn)差異不顯著(P0.05)。綜上表明,臍帶間充質(zhì)干細(xì)胞能夠通過IGF-1促進(jìn)乳腺上皮細(xì)胞乳脂合成及關(guān)鍵基因的表達(dá),JAK2/STAT5信號(hào)通路不參與臍帶間充質(zhì)干細(xì)胞對(duì)乳腺上皮細(xì)胞乳脂調(diào)控。
[Abstract]:To investigate the effects of co-culture of bovine mammary epithelial cells (BMECs) and umbilical cord mesenchymal stem cells (UC-MSCs) on the synthesis of milk fat and expression of key genes in the milk of BMC cells. The experiment was divided into 8 groups: the co-culture group was untreated under the condition of UC-MSCs and BMECs co-culture. IGF-1R inhibitor AG1024 treatment group, AG490 treatment group and AG1024 AG490 treatment group, The control group was treated with BMECs mono-culture. The level of IGF-1R inhibitor AG1024 was detected by RT-qPCR. The signal pathway blocker AG490 and AG1024 AG490 were used to detect the IGF-1 and triglyceride TGs in the supernatant of each group. The relative expression abundance of acetyl coenzyme A carboxylase, fatty acid synthase (FASN) and sterol regulatory element binding proteins1 (SREBP1) gene was observed. The content of IGF-1G in co-cultured group was significantly higher than that in control group (P0.05AG-1024). The effect of P0.01C on IGF-1 was significantly lower than that of P0.01treatment, and the relative expression abundance of ACA FASNNU SREBP1mRNA and the relative expression of ACA FASNN SREBP1 mRNA were not affected by ACA FASNN SREBP1 mRNA expression in ACA FASNSREBP1 treated with ACA FASNN AG1024 and AG490 alone. The results showed that the expression of ACA FASNSREBP1 mRNA was not affected by ACA FASNN AG1024 and AG490 alone. There was no significant difference in the performance of various indexes between the two groups (P 0.05). Umbilical cord mesenchymal stem cells can promote breast epithelial cell milk fat synthesis and expression of key genes through IGF-1. JAK2 / STAT5 signaling pathway does not participate in the regulation of breast epithelial cell milk fat by umbilical cord mesenchymal stem cells.
【作者單位】： 新疆農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)學(xué)院新疆肉乳用草食動(dòng)物營養(yǎng)實(shí)驗(yàn)室;
【基金】：奶產(chǎn)業(yè)體系資助(CARS-37) 國家自然科學(xué)基金(31560645) 中國博士后基金 新疆維吾爾自治區(qū)高等學(xué)�？蒲杏�(jì)劃項(xiàng)目(XJEDU2013S17) 2013年度新疆研究生科研創(chuàng)新項(xiàng)目(xjau-2013-yjsky-XJGRI2013113) 新疆肉乳用草食動(dòng)物營養(yǎng)實(shí)驗(yàn)室開放課題
【分類號(hào)】：S823

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