本文關(guān)鍵詞： 長(zhǎng)柄扁桃 AP 轉(zhuǎn)錄組 低拷貝核基因　出處：《分子植物育種》2017年08期 　論文類(lèi)型：期刊論文

【摘要】：通過(guò)分析長(zhǎng)柄扁桃幼苗葉片轉(zhuǎn)錄組測(cè)序,從中找到一個(gè)包含AP2結(jié)構(gòu)域的Unigene,該Unigene包含完整的開(kāi)放讀碼框,全長(zhǎng)3182bp,編碼767個(gè)氨基酸,將其命名為ApAP2dc1。以ApAP2dc1的DNA序列作為信息探針,在桃和梅的基因組序列中進(jìn)行查詢(xún)比較,發(fā)現(xiàn)桃和梅的基因組中均存在ApAP2dc1的直系同源基因,分別命名為PpAP2dc1和PmAP2dc1。在桃和梅的基因組中還各發(fā)現(xiàn)一個(gè)串聯(lián)重復(fù)的旁系同源基因,分別命名為PpRYdc1和PmRINGdc1。保守結(jié)構(gòu)域分析表明,ApAP2dc1、PpAP2dc1和PmAP2dc1都含有AP2結(jié)構(gòu)域,而PpRYdc1和PmRINGdc1不含AP2結(jié)構(gòu)域;基因結(jié)構(gòu)分析表明,PpAP2dc1和PmAP2dc1、PpRYdc1和PmRINGdc1雖然互為直系同源基因,但其基因結(jié)構(gòu)有明顯差異;旁系同源基因的啟動(dòng)子序列也有較大差異。該研究結(jié)果表明長(zhǎng)柄扁桃、桃和梅基因組上的上述區(qū)域在進(jìn)化上不保守,該區(qū)域的基因在結(jié)構(gòu)和表達(dá)上正在進(jìn)行快速趨異進(jìn)化。
[Abstract]:By analyzing the transcriptome sequencing of leaves of almond seedlings, we found a complete open reading frame containing AP2 domain. The Unigene contains a complete open reading frame, encoding 767 amino acids and named ApAP2dc1. The DNA sequence of ApAP2dc1 was used as the information probe. By comparing the genomic sequences of peach and plum, we found that both peach and plum have direct homologous genes of ApAP2dc1, named PpAP2dc1 and PmAP2dc1.There is also a tandem repeat homologous gene in the genome of peach and plum. They were named PpRYdc1 and PmRINGdc1.The conserved domain analysis showed that both ApAP2dc1PpAP2dc1 and PmAP2dc1 contained AP2 domain, but PpRYdc1 and PmRINGdc1 did not contain AP2 domain, and the gene structure analysis showed that PpAP2dc1 and PmAP2dc1PpRYdc1 and PmRINGdc1 were homologous genes, but their gene structures were obviously different from those of PmAP2dc1 and PmAP2dc1PpAP2dc1 and PmAP2dc1PpR2dc1 and PmAP2dc1 respectively. The results showed that the above regions in the genome of Almond, Peach and Plum were not conserved in evolution, and the genes in this region were undergoing rapid evolution in structure and expression.
【作者單位】： 榆林學(xué)院生命科學(xué)學(xué)院;
【基金】：陜西省教育廳專(zhuān)項(xiàng)科研計(jì)劃(15JK1856) 榆林學(xué)院高學(xué)歷項(xiàng)目(11GK06) 國(guó)家自然科學(xué)基金(3150020131660075)共同資助
【分類(lèi)號(hào)】：Q943.2

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