發(fā)布時(shí)間：2018-02-24 06:39

  本文關(guān)鍵詞： 石榴 UFGT 基因克隆 表達(dá)分析　出處：《西北植物學(xué)報(bào)》2017年04期 　論文類型：期刊論文

【摘要】：利用RT-PCR和RACE方法,從石榴(Punica granatum L.)果皮中克隆到一個(gè)類黃酮糖基轉(zhuǎn)移酶(UFGT)基因(PgUFGT)全長(zhǎng)cDNA序列(GenBank登錄號(hào)為KF841620)。PgUFGT基因編碼區(qū)1 476bp,編碼491個(gè)氨基酸。PgUFGT蛋白具有保守PSPG基序、UDP-糖基轉(zhuǎn)移酶家族結(jié)構(gòu)域和UDP-葡萄糖醛酸基/葡萄糖基轉(zhuǎn)移酶保守域(UDPGT),與其他植物UFGT蛋白一致性較高;系統(tǒng)進(jìn)化樹分析結(jié)果表明,PgUFGT屬于類黃酮3-O-糖基轉(zhuǎn)移酶類。熒光定量qRT-PCR結(jié)果表明,PgUFGT基因在‘紅寶石’和‘水晶甜’2個(gè)石榴品種的發(fā)育期內(nèi)具有不同的表達(dá)模式,PgUFGT在‘紅寶石’石榴中有2個(gè)轉(zhuǎn)錄表達(dá)高峰,而在‘水晶甜’石榴中僅有1個(gè)表達(dá)高峰,表明PgUFGT可能在2個(gè)石榴品種中具有不同的催化作用。該研究結(jié)果為進(jìn)一步研究石榴果實(shí)色澤形成的分子機(jī)制奠定了基礎(chǔ)。
[Abstract]:Using RT-PCR and RACE methods, A full-length cDNA sequence of flavonoid glycosyltransferase (granatum granatum) gene was cloned from the pericarp of Punica granatum L.). GenBank accession number is 1 476 BP, encoding 491 amino acids. PgUFGT protein has conserved PSPG motif and UDP- glycosyltransferase family knot. The conserved domain of UDP-glucuronyl / glucosyltransferase (UDP-glucuronyl / glucosyltransferase) conserved with UFGT protein in other plants. Phylogenetic tree analysis showed that PgUFGT belonged to flavonoid 3-Oglycosyltransferase. Fluorescence quantitative qRT-PCR showed that PgUFGT gene had different expression patterns during the development period of 'ruby' and 'crystal sweet' pomegranate. There were two peaks of transcription expression in 'ruby' pomegranate. However, there was only one peak expression in 'crystal sweet' pomegranate, indicating that PgUFGT may have different catalytic effect in two pomegranate varieties. The results laid a foundation for further study on the molecular mechanism of fruit color formation of pomegranate.
【作者單位】： 南京林業(yè)大學(xué)南方現(xiàn)代林業(yè)協(xié)同創(chuàng)新中心;南京林業(yè)大學(xué)林學(xué)院;山東省果樹研究所;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31272143) 南京林業(yè)大學(xué)高層次人才科研啟動(dòng)基金(GXL2014070) 江蘇省高校優(yōu)勢(shì)學(xué)科建設(shè)工程項(xiàng)目(PAPD)
【分類號(hào)】：Q943.2;S665.4
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本文編號(hào)：1529260