本文關(guān)鍵詞： 含人類(lèi)Ⅰ型血小板結(jié)合蛋白基序的解聚蛋白樣金屬蛋白酶- 單核苷酸多態(tài)性 冠心病　出處：《重慶醫(yī)科大學(xué)學(xué)報(bào)》2016年12期 　論文類(lèi)型：期刊論文

【摘要】：目的:探討ADAMTS1基因SNPrs428785和SNPrs2738兩個(gè)位點(diǎn)與冠心病(coronary heart disease,CHD)的相關(guān)性。方法:采用聚合酶鏈反應(yīng)結(jié)合單鏈構(gòu)象多態(tài)性和聚合酶鏈反應(yīng)結(jié)合限制性片段長(zhǎng)度多態(tài)性,再結(jié)合DNA測(cè)序技術(shù)對(duì)315例CHD患者和355例對(duì)照人群進(jìn)行SNPrs428785和SNPrs2738檢測(cè),2組間計(jì)數(shù)資料比較采用卡方檢驗(yàn),計(jì)量資料比較采用獨(dú)立樣本t檢驗(yàn)和Mann-Whitney U檢驗(yàn)分析。結(jié)果:SNPrs428785在CHD組和對(duì)照組中基因型頻率為CC(50.2%vs 38.9%)、CG(40.3%vs.47.9%)和GG(9.5%vs.13.2%),差異有統(tǒng)計(jì)學(xué)意義(χ~2=8.974,P=0.011),2組間G等位基因頻率分別為29.7%和37.2%,差異有統(tǒng)計(jì)學(xué)意義(χ~2=8.410,P=0.004),經(jīng)二元logistic回歸分析后,提示rs428785的基因型與冠心病的發(fā)生相關(guān)(OR=1.528,95%CI=1.086~2.150,P=0.015)。SNPrs2738在CHD組和對(duì)照組中的基因型頻率為AA(74.3%vs.78.9%)、AC(24.1%vs.18.9%)和CC(1.6%vs.2.3%),差異無(wú)統(tǒng)計(jì)學(xué)意義(χ~2=2.998,P=0.223),A等位基因頻率分別為86.3%和88.3%,差異無(wú)統(tǒng)計(jì)學(xué)意義(χ~2=1.164,P=0.281)。結(jié)論:ADAMTS1基因SNPrs428785多態(tài)性與冠心病發(fā)生相關(guān),且G等位基因可能是冠心病的遺傳易感基因,而SNPrs2738多態(tài)性與冠心病發(fā)生無(wú)相關(guān)性。
[Abstract]:Objective: to investigate the relationship between SNPrs428785 and SNPrs2738 of ADAMTS1 gene and coronary heart disease (CHD). Methods: polymerase chain reaction (PCR) combined with single strand conformation polymorphism (SSCP) and polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) were used. Combined with DNA sequencing technique, the counting data of 315 CHD patients and 355 controls were compared between SNPrs428785 and SNPrs2738 detection group by chi-square test. The quantitative data were analyzed by independent sample t test and Mann-Whitney U test. Results the frequency of G allele in CHD group and control group was CC(50.2%vs 38.9% and CC(50.2%vs 38.9%, respectively. The difference was statistically significant (蠂 2 8.974P0. 011) and G allele frequencies were 29.7% and 37.2%, respectively, and the difference was statistically significant (蠂 2 8.974P0. 011), the difference was significant (蠂 2 8.974 P0. 01111), the frequency of G allele was 29.7% and 37. 2% respectively, and the difference was significant (蠂 2 8.97 4 P 0. 011 1), the difference was significant (蠂 2 8. 97 4 P 0. 011). There was statistical significance (蠂 ~ 2 ~ 2 ~ (8.410) P ~ (0.004)). After binary logistic regression analysis, The results suggest that the genotype of rs428785 is related to the occurrence of coronary heart disease. The frequency of genotype of rs428785 in CHD group and control group is AA74.3vs.78.9s. There is no significant difference between CHD group and control group. Conclusion there is no significant difference in allele frequency of ADAMTS1 gene between CHD group and control group. Conclusion there is no significant difference in allele frequencies of ADAMTS1 gene between CHD group and control group (蠂 ~ 22.998A = 86.3% and 88.323A, respectively). Conclusion there is no significant difference in allele frequencies of ADAMTS1 gene between CHD group and control group (P < 0.01). Conclusion there is no significant difference in allele frequencies of ADAMTS1 gene between CHD group and control group (P < 0.05). Conclusion there is no significant difference in allele frequencies of ADAMTS1 gene between CHD group and control group. SNPrs428785 polymorphism is associated with coronary heart disease. G allele may be a genetic susceptibility gene to coronary heart disease, but SNPrs2738 polymorphism is not associated with coronary heart disease.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院臨床研究中心;
【分類(lèi)號(hào)】：R541.4
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本文編號(hào)：1507991