玉米熱激轉(zhuǎn)錄因子基因ZmHsf06表達(dá)提高擬南芥耐鹽性
本文關(guān)鍵詞: 玉米 ZmHsf 熱激轉(zhuǎn)錄因子 鹽脅迫 基因表達(dá) 出處:《河北農(nóng)業(yè)大學(xué)學(xué)報(bào)》2016年02期 論文類型:期刊論文
【摘要】:在對(duì)從玉米幼葉中獲得熱激轉(zhuǎn)錄因子基因ZmHsf06生物學(xué)特性及轉(zhuǎn)基因擬南芥植株耐熱性和抗旱性評(píng)價(jià)的基礎(chǔ)上,從表型和生理水平對(duì)轉(zhuǎn)ZmHsf06基因植株的耐鹽性進(jìn)行了鑒定。表型觀察結(jié)果顯示,鹽脅迫下轉(zhuǎn)ZmHsf06擬南芥植株根系和地上部長勢(shì)明顯強(qiáng)于野生型;生理指標(biāo)分析發(fā)現(xiàn),鹽脅迫下轉(zhuǎn)基因植株能保持相對(duì)較高的葉片SOD、POD、CAT活性和葉綠素含量,以及相對(duì)較低的相對(duì)電導(dǎo)率、丙二醛含量,且葉片滲透勢(shì)升高明顯。結(jié)果表明,ZmHsf06不僅能提高植株耐熱性和抗旱性,在一定程度上對(duì)提高植株的耐鹽性也發(fā)揮重要作用,是玉米熱激轉(zhuǎn)錄因子家族主要成員之一。
[Abstract]:The biological characteristics of heat shock transcription factor gene (ZmHsf06) obtained from young leaves of maize and the heat tolerance and drought resistance of transgenic Arabidopsis thaliana plants were evaluated. The salt tolerance of transgenic ZmHsf06 transgenic plants was identified by phenotypic and physiological levels. Phenotypic observation showed that the root and shoot potential of transgenic ZmHsf06 Arabidopsis plants under salt stress was significantly stronger than that of wild type. Under salt stress, transgenic plants could maintain relatively high activity of POD POD cat and chlorophyll content in leaves, as well as relatively low relative electrical conductivity and malondialdehyde content. The results showed that ZmHsf06 could not only improve the heat resistance and drought resistance of plants, but also play an important role in improving the salt tolerance of plants to some extent. ZmHsf06 was one of the main members of heat shock transcription factor family in maize.
【作者單位】: 河北省農(nóng)林科學(xué)院遺傳生理研究所/河北省植物轉(zhuǎn)基因中心重點(diǎn)實(shí)驗(yàn)室;石家莊市農(nóng)林科學(xué)研究院;
【基金】:河北省應(yīng)用基礎(chǔ)研究重點(diǎn)研究項(xiàng)目(12965517D)
【分類號(hào)】:S513
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