本文關(guān)鍵詞： 蒙古黃芪 低溫脅迫 異黃酮 植物內(nèi)源激素 差異表達(dá)基因　出處：《植物生理學(xué)報(bào)》2017年04期 　論文類型：期刊論文

【摘要】：溫度是調(diào)控植物生長(zhǎng)發(fā)育和代謝產(chǎn)物積累的重要因素。為了研究溫度調(diào)控蒙古黃芪次生代謝產(chǎn)物積累的分子機(jī)制,本文基于轉(zhuǎn)錄組測(cè)序結(jié)果對(duì)常溫和低溫組的蒙古黃芪幼苗基因表達(dá)水平進(jìn)行對(duì)比分析。結(jié)果表明:相對(duì)于常溫組,低溫組差異表達(dá)基因共3 896個(gè),其中表達(dá)上調(diào)和下調(diào)的基因數(shù)目分別為2 420和1 476個(gè)。關(guān)鍵基因差異表達(dá)分析顯示,低溫組異黃酮代謝途徑中大部分關(guān)鍵基因表達(dá)量都顯著上調(diào),而植物激素合成轉(zhuǎn)導(dǎo)途徑中的關(guān)鍵基因表達(dá)模式各有不同。由此可見,低溫脅迫能夠促進(jìn)蒙古黃芪中部分藥用成分的合成,同時(shí)能改變部分內(nèi)源植物激素的合成和轉(zhuǎn)導(dǎo),從而使其更好的適應(yīng)低溫環(huán)境。
[Abstract]:In order to study the molecular mechanism of temperature regulating the accumulation of secondary metabolites of Astragalus mongolicus (Astragalus mongolicus), temperature is an important factor in regulating plant growth and metabolite accumulation. Based on the results of transcriptome sequencing, the gene expression levels of Astragalus mongolicus seedlings at room temperature and low temperature were compared. The results showed that there were 3 896 differentially expressed genes in low temperature group compared with normal temperature group. The number of genes up-regulated and down-regulated were 2 420 and 1 476 respectively. The differential expression analysis of key genes showed that most of the key genes in isoflavone metabolism pathway were significantly up-regulated in hypothermia group. However, there are different expression patterns of key genes in plant hormone synthesis transduction pathway. Therefore, low temperature stress can promote the synthesis of some medicinal components in Astragalus mongolicus (Astragalus mongolicus). At the same time, it can change the synthesis and transduction of some endogenous plant hormones, so that it can adapt to low temperature environment better.
【作者單位】： 復(fù)旦大學(xué)生命科學(xué)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(8137963)~~
【分類號(hào)】：S567.239
【正文快照】： 收稿2016-12-26修定2017-03-10蒙古黃芪[Astragalus membranaceus Bge.var.mongolicus(Bge.)Hsiao]是我國(guó)最重要的中藥材之一,有廣泛的生理和藥理活性,能增強(qiáng)機(jī)體免疫力,清除自由基,在保護(hù)心肌細(xì)胞、增強(qiáng)心肌活力、抗腫瘤等方面有廣泛應(yīng)用(陳國(guó)輝和黃文鳳2008)。黃芪皂苷(主要

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