發(fā)布時(shí)間：2018-01-29 01:15

  本文關(guān)鍵詞： 大豆銹病 抗銹遺傳 基因定位　出處：《中國(guó)農(nóng)業(yè)科學(xué)院》2016年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：由豆薯層銹菌(Phakopsora pachyrhizi)引起的大豆銹病,已經(jīng)成為世界大豆主產(chǎn)區(qū)主要病害,嚴(yán)重影響大豆產(chǎn)量。與化學(xué)藥物防治相比,培育抗銹品種是控制大豆銹病最為安全有效的途徑。抗銹資源篩選、抗銹基因遺傳分析是開(kāi)展抗病育種和抗病機(jī)理研究的基礎(chǔ)。本研究在前期發(fā)現(xiàn)一份對(duì)銹菌小種SS4表現(xiàn)高抗的大豆資源SX6907的基礎(chǔ)上,開(kāi)展SX6907抗銹遺傳分析及抗銹基因定位研究。主要研究結(jié)果如下:1.抗銹遺傳分析:利用感病品種天隆1號(hào)、中豆40及蒲豆11分別與抗銹親本SX6907雜交,構(gòu)建了三個(gè)F2群體。采用離體葉片接種法,對(duì)親本以及三個(gè)組合的F1和F2單株進(jìn)行抗銹鑒定。結(jié)果表明:三個(gè)組合的F1植株都表現(xiàn)不同于雙親的的紅黑色(Red-black,RB)病斑反應(yīng)型;F2群體中出現(xiàn)3種抗病反應(yīng)型,經(jīng)卡方測(cè)驗(yàn),三種抗病反應(yīng)型分離比例符合1免疫(Immune,IM):2紅黑色病斑(RB):1黃褐色病斑(TAN)的分離比,說(shuō)明抗銹資源SX6907對(duì)銹菌小種SS4的抗性可能是由一對(duì)不完全顯性基因控制。2.抗銹基因初步定位:利用BSA(Bulked Segregation Analysis)法,對(duì)抗、感親本(中豆40、天隆一號(hào)、蒲豆11和SX6907)及抗、感池進(jìn)行多態(tài)性篩選及標(biāo)記連鎖分析,結(jié)果表明18號(hào)染色體上、Rpp1位點(diǎn)附近標(biāo)記與抗銹性狀連鎖。通過(guò)對(duì)Rpp1位點(diǎn)附近標(biāo)記在中豆40×SX6907組合F2群體上的標(biāo)記分析,將抗銹基因初步定位在標(biāo)記BARCSOYSSR_18_1856和BARCSOYSSR_18_1864之間,物理距離約為363.9kb,標(biāo)記分離比例與3種抗病反應(yīng)分離比例一致。利用天隆1號(hào)×SX6907和蒲豆11×SX6907兩個(gè)組合的F2分離群體驗(yàn)證了該結(jié)果。3.抗銹基因精細(xì)定位:進(jìn)一步在初定位區(qū)間內(nèi)開(kāi)發(fā)出多態(tài)性SSR標(biāo)記11個(gè)。利用初步定位側(cè)翼標(biāo)記對(duì)天隆1號(hào)×SX6907雜交獲得的另外800株F2單株進(jìn)行重組子篩選,獲得重組單株16株。采用新開(kāi)發(fā)的標(biāo)記對(duì)重組子進(jìn)行標(biāo)記分析,結(jié)合表型鑒定,最終將抗銹基因定位在標(biāo)記SSR24和SSR40之間,物理距離約為111.9Kb。與已知Rpp1資源的抗銹基因定位區(qū)間和抗性表現(xiàn)進(jìn)行比較,推測(cè)SX6907抗銹基因?yàn)椴煌赗pp1位點(diǎn)的新基因,將其命名為Rpp6907。4.抗銹候選基因分析:對(duì)Rpp6907所在定位區(qū)間內(nèi)基因預(yù)測(cè)顯示,該區(qū)間存在11個(gè)候選基因。其中三個(gè)基因Glyma18g51930、Glyma18g51950和Glyma18g51960屬于NBS-LRR類(lèi)抗病基因家族,推測(cè)它們可能是Rpp6907位點(diǎn)的抗銹候選基因。
[Abstract]:Soybean rust caused by Phakopsora pachyrhizi) has become the main disease in soybean producing areas in the world. Compared with chemical drug control, breeding rust resistant varieties is the safest and effective way to control soybean rust. Genetic analysis of rust resistance genes is the basis of the research on resistance breeding and disease resistance mechanism. This study was based on the discovery of a soybean resource SX6907 with high resistance to rust race SS4. The main results of this study were as follows: 1. Genetic analysis of rust resistance: using the susceptible cultivar Tianlong 1. Three F2 populations were constructed by crossing Zhongdou 40 and Pudou 11 with rust resistant parent SX6907, respectively. The method of leaf inoculation in vitro was used. The rust resistance of the F1 and F2 plants of their parents and three combinations were identified. The results showed that all the F1 plants of the three combinations showed Red-black which was different from their parents. RBB); In F2 population, there were three resistant response types, and the segregation ratio of the three disease resistance response types was in accordance with 1 immunized Immune by chi-square test. The isolation ratio of IM):2, RBC: 1. It is suggested that the resistance of rust resistant resource SX6907 to SS4 may be controlled by a pair of incomplete dominant genes. Bulked Segregation Analysis. Antagonistic, susceptible parents (Zhongdou 40, Tianlong 1, Pudou 11 and SX6907) and resistance, susceptibility pool were screened by polymorphic analysis and marker linkage analysis. The results showed that chromosome 18 was on chromosome 18. Rpp1 locus markers were linked to rust resistance traits. The markers near Rpp1 locus were analyzed on F _ 2 population of Zhongdou 40 脳 SX6907 combination. The rust resistance gene was preliminarily located between the labeled BARCSOYSSR_18_1856 and BARCSOYSSR_18_1864, and the physical distance was about 363.9 kb. The segregation ratio of marker was the same as that of three disease resistance reactions. The results were verified by F2 population of Tianlong 1 脳 SX6907 and Pudou 11 脳 SX6907. 3. The antirust gene spermatozoa was obtained. Fine positioning:. Furthermore, 11 polymorphic SSR markers were developed in the initial locational region. The recombinant clones of another 800 F2 plants obtained from Tianlong 1 脳 SX6907 hybridization were screened by using the preliminary flanking marker. A total of 16 recombinant single strains were obtained. The newly developed markers were used for marker analysis and phenotypic identification. Finally, the rust resistance gene was located between SSR24 and SSR40. The physical distance is about 111.9 KB. Compared with the known Rpp1 resources, we speculated that the rust-resistant gene of SX6907 was different from the new gene at Rpp1 site. Rpp6907.4. Rpp6907.4. Rpp6907.4. Rpp6907.4.Analysis of rust resistance candidate genes: prediction of genes. There are 11 candidate genes in this region, three of which are Glyma18g51930. Glyma18g51950 and Glyma18g51960 belong to the family of NBS-LRR resistance genes, which may be the candidate genes of Rpp6907 locus.
【學(xué)位授予單位】：中國(guó)農(nóng)業(yè)科學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：S435.651

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本文編號(hào)：1472090