本文關(guān)鍵詞： 結(jié)直腸癌 pINKa基因 MGMT基因 RASSFA基因 基因甲基化　出處：《山東醫(yī)藥》2017年31期 　論文類型：期刊論文

【摘要】：目的觀察結(jié)直腸癌(CRC)患者糞便p16INK4a、MGMT、RASSF1A基因甲基化狀態(tài)。方法 CRC患者50例(腫瘤組)、結(jié)直腸良性病變者50例(良性組)、健康體檢者50例(正常組),采用甲基化特異度PCR的方法檢測(cè)各組糞便p16INK4a、MGMT、RASSF1A基因甲基化檢出率,并分析其與CRC臨床病理參數(shù)的關(guān)系。根據(jù)腸鏡病理診斷結(jié)果進(jìn)行驗(yàn)證,比較糞便p16INK4a、MGMT、RASSF1A單獨(dú)及聯(lián)合檢測(cè)診斷CRC的敏感度和特異度。結(jié)果腫瘤組、良性組、正常組糞便p16INK4a基因甲基化檢出率分別為74%、28%、14%,MGMT基因甲基化檢出率分別為56%、24%、12%,RASSF1A基因甲基化檢出率分別為72%、20%、10%,腫瘤組分別與正常組、良性組比較,P均0.05。p16INK4a基因甲基化狀態(tài)與CRC分化程度、TNM分期、淋巴結(jié)轉(zhuǎn)移相關(guān),MGMT基因甲基化狀態(tài)與CRC淋巴結(jié)轉(zhuǎn)移相關(guān),RASSF1A基因甲基化狀態(tài)與CRC分化程度、TNM分期、淋巴結(jié)轉(zhuǎn)移相關(guān),P均0.05。三者聯(lián)合檢測(cè)診斷CRC的敏感度為95.8%,特異度為83.4%,ROC曲線下面積為0.816(95%CI 0.739%~0.894%)。結(jié)論 CRC患者糞便p16INK4a、MGMT、RASSF1A基因甲基化檢出率明顯高于結(jié)直腸良性病變者及健康體檢者,聯(lián)合檢測(cè)上述指標(biāo)有助于CRC患者的早期診斷及其生物學(xué)行為的判斷。
[Abstract]:Objective to observe the methylation status of p16INK4a MGMT-RASSF1A gene in feces of patients with colorectal cancer. Methods 50 patients with CRC (tumor group) were enrolled in this study. Fifty patients with colorectal benign lesions (benign group) and 50 healthy persons (normal group) were detected by methylation specificity (PCR) method. The detection rate of methylation of RASSF1A gene and its relationship with the clinicopathological parameters of CRC were analyzed. The stool p16INK4a MGMT was compared according to the results of pathological diagnosis under enteroscopy. Results the detection rate of methylation of p16INK4a gene in feces of tumor group, benign group and normal group was 7428%. The positive rate of methylation of MGMT gene was 56%, and the positive rate of methylation of RASSF1A gene was 72%, 20% and 10%, respectively. The methylation status of p16INK4a gene was correlated with CRC differentiation and lymph node metastasis. The methylation status of MGMT gene was associated with CRC lymph node metastasis. The methylation status of RASSF1A gene was correlated with the degree of CRC differentiation and lymph node metastasis. P all 0.05. The sensitivity and specificity of combined detection and diagnosis of CRC were 95.8 and 83.4% respectively. The area under the ROC curve was 0.816 ~ 95% CI 0.739 and 0.8944.Conclusion the stool p16INK4a of CRC patients has MGMT. The detection rate of methylation of RASSF1A gene was significantly higher than that of benign colorectal lesions and healthy persons. The combined detection of the above indexes was helpful to the early diagnosis and the judgement of biological behavior of patients with CRC.
【作者單位】： 惠州市中心人民醫(yī)院;
【基金】：廣東省惠州市民政局資助項(xiàng)目(20151116344)
【分類號(hào)】：R735.34
【正文快照】： 結(jié)直腸癌(CRC)是常見的惡性消化道腫瘤之一,有較高的發(fā)病率及病死率[1,2]。CRC早期未發(fā)生癌組織轉(zhuǎn)移時(shí)患者5年生存率為90%,發(fā)生局部轉(zhuǎn)移時(shí)患者生存率為68%,發(fā)生遠(yuǎn)處轉(zhuǎn)移時(shí)患者生存率僅為10%[3~5]。早發(fā)現(xiàn)及早治療能明顯提高CRC患者的生存率[6],近年發(fā)達(dá)國(guó)家的CRC患者5年生存率

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