發(fā)布時間：2018-01-24 18:57

  本文關(guān)鍵詞： 腺病毒載體 裸鼠 Fat-基因 Caspase- 子宮內(nèi)膜癌　出處：《現(xiàn)代婦產(chǎn)科進展》2017年05期 　論文類型：期刊論文

【摘要】：目的:探究腺病毒介導(dǎo)的Fat-1基因(Ad-GFP-Fat-1)對人子宮內(nèi)膜癌Ishikawa細胞體內(nèi)抑制效應(yīng)及其潛在的分子機制。方法:體外培養(yǎng)人子宮內(nèi)膜癌Ishikawa細胞,接種于裸鼠右前肢腋部,建立人子宮內(nèi)膜癌裸鼠皮下移植瘤模型。將成瘤后裸鼠隨機分成3組:Ad-GFP-Fat-1實驗組、Ad-GFP空載體組、PBS對照組,分別對裸鼠進行皮下移植瘤注射治療。觀察并測量瘤體體積。RT-PCR檢測Fat-1基因表達,TUNEL+DAPI染色法檢測移植瘤組織凋亡,免疫組化SP法和Western blot檢測瘤體內(nèi)半胱氨酸蛋白酶(Caspase)-3的表達情況。結(jié)果:Fat-1基因在Ad-GFP-Fat-1組移植瘤組織中有效異源表達。Ad-GFP-Fat-1組裸鼠移植瘤的體積和重量明顯小于Ad-GFP組和PBS組(瘤體比較:P0.05,瘤重比較:P0.05)。Ad-GFP-Fat-1組的凋亡指數(shù)明顯高于Ad-GFP組和PBS組(P0.05)。Ad-GFP-Fat-1可明顯上調(diào)Caspase-3表達。Ad-GFP-Fat-1主要通過增加活化的Caspase-3(Cleaved Caspase-3)上調(diào)Caspase-3表達。結(jié)論:Ad-GFP-Fat-1表達對人子宮內(nèi)膜癌裸鼠皮下移植瘤有抑制作用,其機制可能與Fat-1基因上調(diào)Cleaved Caspase-3表達誘導(dǎo)細胞凋亡有關(guān)。
[Abstract]:Objective: to investigate the adenovirus mediated Fat-1 gene Ad-GFP-Fat-1. Methods: human endometrial carcinoma Ishikawa cells were cultured in vitro. The nude mice were inoculated into the armpit of right forelimb of nude mice and subcutaneously transplanted tumor models of human endometrial carcinoma were established. The nude mice were randomly divided into 3 groups: 1: Ad-GP-Fat-1 experimental group and Ad-GFP empty carrier group. In PBS control group, subcutaneous tumor transplantation was performed in nude mice, and tumor volume was measured. RT-PCR was used to detect the expression of Fat-1 gene in nude mice. Apoptosis of transplanted tumor was detected by TUNEL DAPI staining. Detection of cysteine protease caspase in tumor by immunohistochemical SP method and Western blot. Results:. The volume and weight of Fat-1 gene in xenografts in Ad-GFP-Fat-1 group were significantly lower than those in Ad-GFP group and that in Ad-GFP-Fat-1 group was significantly lower than that in Ad-GFP group. PBS group (. Tumor comparison: P0.05. The apoptotic index was significantly higher in the tumor weight group than in the Ad-GFP group and the PBS group (P 0.05). The apoptotic index in the Ad-GFP-Fat-1 group was significantly higher than that in the Ad-GFP group and the PBS group. Ad-GFP-Fat-1 significantly upregulated the expression of Caspase-3. Ad-GFP-Fat-1 mainly increased the activated Caspase-3 (Caspase-3). Conclusion the expression of Cleaved Caspase-3 up-regulates the expression of Caspase-3. Conclusion the expression of Cleaved-GFP-Fat-1 can inhibit the subcutaneous transplantation of human endometrial carcinoma in nude mice. The mechanism may be related to the up-regulation of Cleaved Caspase-3 expression by Fat-1 gene.
【作者單位】： 徐州醫(yī)科大學(xué)研究生學(xué)院婦產(chǎn)科;
【基金】：江蘇省衛(wèi)生廳醫(yī)學(xué)科研項目(No:H201428)
【分類號】：R737.33
【正文快照】： 子宮內(nèi)膜癌是女性生殖系統(tǒng)三大惡性腫瘤之一[1],占女性生殖系統(tǒng)惡性腫瘤的20%~30%[2]。目前,子宮內(nèi)膜癌的治療手段十分有限,迫切需要具體的、有效的、無毒的治療方法。大量研究表明,n-6/n-3多不飽和脂肪酸(polyunsaturated fatty acids,PUFAs)的比例與腫瘤的發(fā)生發(fā)展有關(guān)。通，

本文編號：1460771