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  本文關鍵詞： 小麥(Triticum aestivum L.) 分蘗角度 TaTAC 同源克隆 表達分析　出處：《植物遺傳資源學報》2017年01期 　論文類型：期刊論文

【摘要】：分蘗角度影響植物群體結構、光合效率以及植株的形態(tài)建成,最終影響其產量及品質。小麥中關于分蘗角度的研究鮮有報道。前人研究顯示,水稻OsTAC1正向調控分蘗角度的大小,玉米Zm TAC1與葉片角度呈正相關。本研究的目的是解析Ta TAC1的表達模式并初步了解該基因的分子遺傳機制及其與分蘗角度的遺傳關系。本研究以CN16、SM969、Lan2399、SHW-1為材料,利用同源克隆分離獲得Ta TAC1,利用生物信息學軟件對Ta TAC1進行序列特征分析,應用實時熒光定量PCR對其表達模式進行分析,并進行Ta TAC1蛋白的亞細胞定位分析。結果顯示,Ta TAC1長度約1.1~1.2 kb,包括780 bp的完整開放閱讀框和320~370 bp的3'-UTR。Ta TAC1的c DNA序列分為2類,其中CN16-2、SM969-2的第10號堿基發(fā)生突變,引起終止子提前,導致Ta TAC1表達受阻;Lan2399-2和SHW-1-2在109~115堿基位置有"CGCGCG"片段插入,導致蛋白質β-折疊片減少。表達分析表明,Ta TAC1在分蘗期的葉鞘、莖高效表達,分蘗節(jié)其次,葉與根表達量最低。相關分析表明該基因在分蘗節(jié)各時期表達量與分蘗角度呈顯著正相關,Pearson相關系數為0.677,其他組織表達量與分蘗角度無顯著相關性。亞細胞定位分析顯示Ta TAC1定位于細胞膜。從上述結果可以推測Ta TAC1在mRNA水平上正向調控分蘗角度,且可能參與生長素極性運輸過程從而改變分蘗角度大小。
[Abstract]:Tiller angle affects plant population structure, photosynthetic efficiency and plant morphogenesis, and ultimately affects yield and quality. There are few reports on tiller angle in wheat. OsTAC1 positively regulated the tiller angle. Zm TAC1 was positively correlated with leaf angle. The expression pattern of TAC1 and the molecular genetic mechanism of the gene and its genetic relationship with tiller angle were preliminarily understood. CN16 was used in this study. Ta TAC-1 was isolated from SM969 / Lan2399 / SHW-1 by homologous cloning and sequenced by bioinformatics software. The expression pattern of Ta TAC1 protein was analyzed by real-time fluorescence quantitative PCR and the subcellular localization of Ta TAC1 protein was analyzed. The results showed that the length of Ta TAC1 was about 1.1 ~ 1.2kb. The complete open reading frame (ORAF) of 780bp and the c DNA sequence of 3GUTR.Ta TAC1 (320,370bp) were classified into two categories, including CN16-2. The mutation of the 10th base of SM969-2 caused the Terminator to advance and the expression of Ta TAC1 was blocked. The insertion of "CGCGCG" fragment of Lan2399-2 and SHW-1-2 at the 109N115base site resulted in the decrease of protein 尾 -folding tablets. Ta TAC1 was highly expressed in leaf sheath and stem at tillering stage, followed by tiller node and lowest in leaf and root. Correlation analysis showed that the expression of Ta TAC1 was positively correlated with tiller angle at each stage of tillering. Pearson correlation coefficient was 0. 677. There was no significant correlation between the expression of other tissues and tiller angle. Subcellular localization analysis showed that Ta TAC1 was located on cell membrane. From the above results, we can infer that Ta is located on cell membrane. The tiller angle was positively regulated by TAC1 at mRNA level. And it may be involved in the polar transport process of auxin, thus changing the tiller angle.
【作者單位】： 四川農業(yè)大學小麥研究所分子生物學實驗室;
【基金】：四川省科技支撐計劃(2016NZ0057) 國家自然科學基金(31301317)
【分類號】：S512.1
【正文快照】： URL:http://www.cnki.net/kcms/detail/11.4996.S.20161214.1435.026.html小麥(Triticum aestivum L.)是世界一半以上人口的主要糧食,其種植面積、總產量以及總貿易額均居各類作物之首。合理的小麥株型可較大幅度提高產量,而分蘗角度是決定株型的主要因素之一,研究與之相關的

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