發(fā)布時間：2018-01-20 15:20

  本文關(guān)鍵詞： 丹參 DIR蛋白 類DIR蛋白 基因家族 生物信息學(xué)　出處：《基因組學(xué)與應(yīng)用生物學(xué)》2017年04期 　論文類型：期刊論文

【摘要】：Dirigent(DIR)蛋白基因是植物木質(zhì)素合成過程中一類重要的基因,幾乎存在于所有維管植物中,然而藥用植物丹參中的DIR和類DIR蛋白基因(SmDIR)還沒有被研究過。本研究通過生物信息學(xué)方法,對已在Gen Bank上登錄的9個丹參轉(zhuǎn)錄組數(shù)據(jù)庫進(jìn)行整合拼接分析,發(fā)現(xiàn)了33個SmDIR基因片段,其中5條為完整序列。通過對5條完整SmDIR基因的序列特征、理化性質(zhì)、跨膜結(jié)構(gòu)域、信號肽、導(dǎo)肽、疏水性/親水性、蛋白質(zhì)二級結(jié)構(gòu)、三級結(jié)構(gòu)及結(jié)構(gòu)域等進(jìn)行預(yù)測分析,并將丹參DIR蛋白序列與其它植物的DIR蛋白進(jìn)行同源比對,構(gòu)建了丹參和模式植物擬南芥DIR蛋白家族的系統(tǒng)進(jìn)化樹。結(jié)果表明,這5條丹參DIR基因的開放閱讀框都在594 bp上下,分子量為21.47 k D左右。除了SmDIR5,其余SmDIR蛋白的理論等電點(diǎn)(p I)均低于7,在5.60~6.50范圍內(nèi),說明大多數(shù)丹參的DIR蛋白略呈酸性。Thr、Val、Gly、Leu和Ser等氨基酸是這些SmDIR蛋白共有的主要氨基酸。研究還發(fā)現(xiàn)這些丹參DIR蛋白表現(xiàn)出明顯的疏水區(qū)和親水區(qū),均存在分泌途徑信號肽,可能存在跨膜結(jié)構(gòu)域。蛋白質(zhì)二級結(jié)構(gòu)中最主要的結(jié)構(gòu)元件是無規(guī)則卷曲。氨基酸同源性比對顯示,SmDIR4與Erythranthe guttata的類DIR蛋白同源性較高,SmDIR1、SmDIR2、SmDIR3和SmDIR5與芝麻的類DIR蛋白同源性較高。與擬南芥的進(jìn)化分析顯示,這些丹參DIR蛋白基因被分為兩大類,歸屬DIR-b和DIR-d亞家族。本研究對丹參DIR蛋白序列特征進(jìn)行了系統(tǒng)分析,可為今后深入研究該蛋白提供有利參考。
[Abstract]:Dirigent-DIRs are important genes in the process of lignin synthesis in plants, which are found in almost all vascular plants. However, the DIR and DIR like protein gene of Salvia miltiorrhiza (Salvia miltiorrhiza) have not been studied. In this paper, 33 SmDIR gene fragments were found in 9 transcriptional database of Salvia miltiorrhiza recorded on Gen Bank. Five of the five complete SmDIR genes were sequenced, including their sequence characteristics, physicochemical properties, transmembrane domain, signal peptide, conducting peptide, hydrophobic / hydrophilic, protein secondary structure. The tertiary structure and domain were predicted and the sequence of DIR protein of Salvia miltiorrhiza was compared with DIR protein of other plants. Phylogenetic tree of DIR protein family of Salvia miltiorrhiza and model plant Arabidopsis thaliana was constructed. The results showed that the open reading frame of DIR gene of Salvia miltiorrhiza was about 594bp. The molecular weight of SmDIR protein was 21.47 KD. Except SmDIR5, the theoretical isoelectric point (Pi) of SmDIR protein was lower than 7, in the range of 5.60 ~ 6.50. The results showed that the DIR protein of most Salvia miltiorrhiza was slightly acidic. Amino acids such as Leu and Ser are the main amino acids shared by these SmDIR proteins. It was also found that the DIR proteins of Salvia miltiorrhiza showed obvious hydrophobic and hydrophilic regions, and there were signal peptides secreting pathway. There may be transmembrane domain. The most important structural element in protein secondary structure is irregular curl. Amino acid homology ratio shows. The homology of DIR like protein between SmDIR4 and Erythranthe guttata is higher than that of SmDIR1 and SmDIR2. SmDIR3 and SmDIR5 share high homology with DIR like proteins of sesame seeds. Evolutionary analysis with Arabidopsis thaliana showed that these DIR genes of Salvia miltiorrhiza were classified into two categories. DIR-b and DIR-d subfamily. The sequence characteristics of DIR protein of Salvia miltiorrhiza were systematically analyzed, which could provide a useful reference for the further study of this protein.
【作者單位】： 中國人民解放軍第二軍醫(yī)大學(xué)藥學(xué)院藥用植物學(xué)教研室;中國人民解放軍第二軍醫(yī)大學(xué)附屬上海長征醫(yī)院藥學(xué)部;
【基金】：國家自然科學(xué)基金青年科學(xué)基金項(xiàng)目(81303160);國家自然科學(xué)基金國家杰出青年科學(xué)基金項(xiàng)目(81325024) 第二軍醫(yī)大學(xué)本科學(xué)員創(chuàng)新實(shí)踐能力孵化基地項(xiàng)目(FH2015095,FH2015096,FH2016081)共同資助
【分類號】：Q943.2;S567.53
【正文快照】： 200003*同等貢獻(xiàn)作者**通訊作者,qli@smmu.edu.cn;zhanglei@smmu.edu.cnDiscovery and Bioinformatics Analysis of Dirigent Multigene Family inSalvia miltiorrhizaMa Jinyang1*Yang Jindong1*Li Qing2**Zhang Lei1**1 Department of Pharmaceutical Botany,School of Phar，

本文編號：1448686