本文關(guān)鍵詞：OsEXPA8基因?qū)λ緫腋〖?xì)胞的周期和大小的影響　出處：《農(nóng)業(yè)生物技術(shù)學(xué)報(bào)》2016年01期 　論文類型：期刊論文

  更多相關(guān)文章： 水稻 膨脹素 OsEXPA 細(xì)胞周期 細(xì)胞大小 流式細(xì)胞儀

【摘要】：膨脹素是植物細(xì)胞特有的一種細(xì)胞壁松弛蛋白,在很大程度上決定著植物細(xì)胞的生長速率。α-膨脹素(α-expansin,EXPA)和β-膨脹素(β-expansin,EXPB)是植物膨脹素家族中兩個(gè)較大的亞家族。水稻(Oryza sativa)中至少有34個(gè)α-膨脹素基因。本研究通過轉(zhuǎn)基因技術(shù),研究了一個(gè)水稻根特異性表達(dá)的α-膨脹素基因Os EXPA8對(duì)水稻懸浮細(xì)胞的細(xì)胞周期和細(xì)胞大小的影響。利用流式細(xì)胞儀,分析不同培養(yǎng)時(shí)期野生型和轉(zhuǎn)基因型細(xì)胞周期的差異,結(jié)果發(fā)現(xiàn),35S::Os EXPA8(過表達(dá))轉(zhuǎn)基因懸浮細(xì)胞大部分處于G2/M期,而Os EXPA8-RNAi(knock-down)轉(zhuǎn)基因懸浮細(xì)胞多處在G1期;利用血球計(jì)數(shù)板測(cè)定細(xì)胞數(shù)量,結(jié)果表明,35S::Os EXPA8轉(zhuǎn)基因細(xì)胞數(shù)量野生型細(xì)胞數(shù)量Os EXPA8-RNAi轉(zhuǎn)基因細(xì)胞數(shù)量;利用顯微鏡測(cè)定不同培養(yǎng)時(shí)期的細(xì)胞大小,發(fā)現(xiàn)35S::Os EXPA8轉(zhuǎn)基因細(xì)胞大小野生型細(xì)胞大小Os EXPA8-RNAi轉(zhuǎn)基因細(xì)胞大小;測(cè)定愈傷組織塊大小,發(fā)現(xiàn)35S::Os EXPA8轉(zhuǎn)基因愈傷組織塊大小野生型愈傷組織塊大小Os EXPA8-RNAi轉(zhuǎn)基因愈傷組織塊大小。本研究結(jié)果表明,Os EXPA8能夠促進(jìn)水稻懸浮細(xì)胞的分裂和生長,從而增加細(xì)胞數(shù)量,增大細(xì)胞體積,對(duì)水稻懸浮細(xì)胞的生長具有調(diào)控作用。該研究將有助于更好地了解植物細(xì)胞壁的松弛過程,從而深入地認(rèn)識(shí)植物的生長發(fā)育進(jìn)程。同時(shí),膨脹素作為一種細(xì)胞壁松弛因子在農(nóng)業(yè)上具有潛在的生物工程應(yīng)用價(jià)值。
[Abstract]:Expansin is a cell wall loosening proteins in plant cell specific, largely determines the growth rate of plant cells. Alpha expansin (alpha -expansin, EXPA) and beta expansin (beta -expansin, EXPB) is a plant expansion in two large family of rice (Oryza sativa subfamily.) at least 34 alpha expansin genes. This study by transgenic technology, studies on an expression of rice root specific alpha expansion effect of Os gene on cell cycle of EXPA8 in rice suspension cell and cell size. Using flow cytometry, analysis of differences in different culture period of wild type and transgenic type the cell cycle was found: 35S: Os EXPA8 (expression) of most transgenic suspension cells in G2/M phase, and Os EXPA8-RNAi (knock-down) transgenic suspension cells in G1 phase; Determination of cell number using hemocytometer. The results showed that: 35S: Os EXPA The number of cell number 8 transgenic wild type cell number of Os transgenic EXPA8-RNAi cells; Determination of different incubation times with microscope cell size, 35S:: Os EXPA8 transgenic cell size of wild type cell size Os EXPA8-RNAi transgenic cell size; Determination of callus size, block size: 35S: block found in wild type callus Os EXPA8 transgenic callus block size Os EXPA8-RNAi transgenic callus block size. The results of this study show that Os EXPA8 can promote the proliferation and growth of rice suspension cells, thereby increasing the number of cells, increase in cell volume, has effects on rice suspension cell growth. The study will contribute to a better understanding of the relaxation process of plant cell wall, thus in-depth understanding the process of growth and development of plants. At the same time, expansion as a cell wall relaxation factor is a potential student in agriculture The application value of the material engineering.

【作者單位】： 重慶大學(xué)生物工程學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(No.31271685) 重慶市自然科學(xué)基金項(xiàng)目(No.cstc2014jcyja80017)
【分類號(hào)】：S511
【正文快照】： 植物生長過程中,位于細(xì)胞壁上的膨脹素使細(xì)胞壁膨脹,促進(jìn)細(xì)胞伸長(Mcqueen-Mason et al.,1992)。膨脹素最初在黃瓜(Cucumis sativus)下胚軸細(xì)胞的胞壁中發(fā)現(xiàn)(Cosgrove et al.,1997;Lee et al.,2001)。隨后,膨脹素基因也相繼在水稻、擬南芥(Arabidopsis thaliana)、大豆(Glycin

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