71沉默CCR和CAD基因培育低木質(zhì)素含量轉(zhuǎn)基因多年生黑麥草
本文關(guān)鍵詞:沉默CCR和CAD基因培育低木質(zhì)素含量轉(zhuǎn)基因多年生黑麥草,由筆耕文化傳播整理發(fā)布。
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第22卷第5期草業(yè)學(xué)報2013年83
Geneticimrovementofrerasswithlowlinincontenterennial 。穑纾纾
bsilencinofCCRandCADenes 。纾纭
1,21,211,21,,HU。耍澹伲粒危兀酰澹妫澹睿蹋桑模幔睿裕粒危牵兀椋幔铮澹椋伲粒危恰。龋铮睢。 。怼,g,g,
111,,WANG。伲幔睿模牛危恰。龋铮睿停痢。兀椋睿颍铮睿酰幔睿纾纾
(,,;1.ChenduInstituteofBioloChineseAcademofSciencesChendu610041,China 。纾纾纭
,)2.GraduateUniversitofChineseAcademofSciencesBeiin100049,China 。辏纭
:,AbstractLinin,oneofthemaincomonentsinvascularlantsismainlresentinthewallsofsecondaril 。纾穑穑穑
,,thickenedcells.Howeveritisalimitinfactorinanumberofaroindustrialsuchaschemicalrocessesul 。 。纾纾穑稹
,,foraediestibilitandlinocellulosictobioethanolconversion.CinnamolCoAreductase(CCR)andin 。 。纾纾纾穑
enultimatecinnamlalcoholdehdroenase(CAD)aretwokeenzmesthatcatalsetheandultimatestes 。纾穑稹
resectivelinthebiosnthesisofmonolinols.BasedontheCCRandCADgseuencesofeneerennial 。穑纾瘢稹
,Lolium。澹颍澹睿睿澹妫颍铮怼。危茫拢桑螅澹悖椋妫椋悖鳎澹颍澹洌澹螅椋睿澹洌簦铮幔洌洌幔颍铮颍椋幔簦澹颍澹螅簦颍椋悖簦椋铮睿澹睿恚澹颍椋恚澹颍螅颍澹颍幔螅螅ā 。穑纾穑穑穑穑纾
sitestoamlifandclonethetwoframentsfromcDNAofrerass.TwoRNAinterferenceeneerennial 。穑纾纾纾稹
()RNAivectors(iCCRandiCAD)wereconstructedusininversereeatframents.Theconstructs2323- 。 。纾穑纾穑稹
wereintroducedintoembroeniccallioferennialrerassbArobacterium-mediatedtransformationwith 。纾穑纾纭
EHA105.ThearomomcinwerescreenedusinanddetectedbPCR,andindeendentiCCRandiCAD 。 。穑纾稹
lantswereobtained.Analsisofrelativelinincontentwasconductedbconventionalmethods.transenic 。穑纾纭
Thelinincontentwasdistinctlreducedcomaredwiththatofwildcontrolsin9plantsofiCCRand11plants 。 。纾稹
ofiCADtranseniclinincontentoftheseiCCRandiCADwasreducedb34.67%andlants.Thelants。 。 。 。纾纾穑稹
33.86%resectivel.Thelantsrew。簦颍幔睿螅澹睿椋悖瑁幔洌睿铮颍恚幔欤恚铮颍瑁铮欤铮幔睿洌鳎澹欤欤裕瑁椋螅螅簦酰洌螅瑁铮鳎澹洌簦瑁幔簟 。穑穑纾纾穑纾
linincontentrerasscouldbeobtainedbsilencinCCRandCADgexressionandhaslowerennialenero-- 。纾纾纾穑穑稹
vidednovelermlasmsforbreedindiestibleandimrovedabsortionrerass. 。纾穑纾纾穑穑纭
:;;LKewordslinin;cinnamolCoAreductase(CCR)cinnamlalcoholdehdroenase(CAD)olium。濉 。纾纾穑
;renne;RNAitransformationenetic。
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本文關(guān)鍵詞:沉默CCR和CAD基因培育低木質(zhì)素含量轉(zhuǎn)基因多年生黑麥草,由筆耕文化傳播整理發(fā)布。
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