【摘要】：目的:探討長鏈非編碼H19靶向調(diào)節(jié)miR-107通過Notch通路對肺癌的侵襲遷移的影響情況。方法:q PCR檢測肺癌和癌旁組織、不同肺癌細(xì)胞中H19的表達(dá)情況;Transwell侵襲實(shí)驗(yàn)檢測沉默H19后肺癌細(xì)胞侵襲能力的變化;劃痕實(shí)驗(yàn)檢測沉默H19后肺癌細(xì)胞遷移能力的變化;雙熒光素酶報(bào)告基因檢測H19與miR-107的相互作用;q PCR檢測肺癌和癌旁組織、不同肺癌細(xì)胞中miR-107的表達(dá)情況;Transwell侵襲實(shí)驗(yàn)檢測沉默H19后miR-107對肺癌細(xì)胞侵襲能力的影響;劃痕實(shí)驗(yàn)檢測沉默H19后miR-107肺癌細(xì)胞遷移能力的影響;裸鼠皮下成瘤檢測沉默H19后miR-107對肺癌成瘤大小以及體積的影響。Western blot檢測沉默H19后Notch通路蛋白的表達(dá)情況。結(jié)果:與癌旁組織相比,肺癌組織中H19表達(dá)明顯增高;肺癌細(xì)胞A549中H19表達(dá)水平最高;沉默H19可以抑制肺癌細(xì)胞侵襲和遷移能力;H19能與miR-107的3'UTR特異性結(jié)合;與癌旁組織相比,肺癌組織中miR-107表達(dá)明顯降低;沉默H19后,抑制miR-107可以促進(jìn)肺癌細(xì)胞侵襲和遷移能力;與H19-siRNA組相比,H19-siRNA+miR-107-inhibitor組荷瘤小鼠腫瘤體積和重量都明顯增大。沉默H19后Notch通路蛋白表達(dá)情況相應(yīng)下調(diào)。結(jié)論:H19在肺癌發(fā)生發(fā)展過程中起重要作用,H19可以靶向調(diào)節(jié)miR-107通過Notch信號(hào)通路調(diào)控肺癌細(xì)胞的侵襲和遷移能力。
[Abstract]:Aim: to investigate the effect of long-chain non-coding H19 target-regulated miR-107 on invasion and migration of lung cancer through Notch pathway. Methods: q PCR was used to detect the expression of H19 in lung cancer tissues and different lung cancer cells, Transwell invasion test was used to detect the invasion ability of lung cancer cells after silencing H19, scratch test was used to detect the changes of migration ability of lung cancer cells after silencing H19. The interaction between H19 and miR-107 was detected by double luciferase reporter gene.; q PCR was used to detect the expression of miR-107 in lung cancer and paracancerous tissues, and Transwell invasion assay was used to detect the effect of miR-107 on invasion ability of lung cancer cells after silencing H19. The migration ability of miR-107 lung cancer cells after silencing H19 was detected by scratch assay, and the effect of miR-107 on tumor size and volume after H19 silencing was detected in nude mice. Western blot was used to detect the expression of Notch pathway protein after H19 silencing. Results: compared with the paracancerous tissues, the expression of H19 in lung cancer tissues was significantly higher than that in lung cancer tissues, the expression of H19 in A549 cells was the highest, the ability of invasion and migration of lung cancer cells was inhibited by silencing H19, and H19 could specifically bind to 3'UTR of miR-107. Compared with the adjacent tissues, the expression of miR-107 in lung cancer tissues was significantly decreased, and the inhibition of miR-107 could promote the invasion and migration of lung cancer cells after silencing H19. Compared with H19-siRNA group, the tumor volume and weight in H19-siRNA miR-107-inhibitor group increased significantly. The expression of Notch pathway protein was down-regulated after H19 silencing. Conclusion: H19 plays an important role in the carcinogenesis and development of lung cancer. H19 can target-regulate miR-107 through Notch signaling pathway to regulate the invasion and migration of lung cancer cells.
【作者單位】： 河南科技大學(xué)第一附屬醫(yī)院胸外科;河南科技大學(xué)第一附屬醫(yī)院風(fēng)濕科;
【分類號(hào)】：R734.2

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