【摘要】：目的建立一種用射頻加熱(radiofrequency heat, RFH)來增強(qiáng)前列腺癌局部化療療效的新的介入治療方法。材料和方法本實(shí)驗(yàn)分為體內(nèi)實(shí)驗(yàn)和體外實(shí)驗(yàn)兩部分。體外實(shí)驗(yàn)部分：將體外培養(yǎng)的人前列腺癌細(xì)胞株分為4組：(1)對照組；(2)僅射頻加熱組；(3)僅化療組(多西紫杉醇)；(4)射頻加熱-化療聯(lián)合治療組。各組細(xì)胞經(jīng)不同治療方法處理后,MTS比色法觀察對比射頻加熱-化療聯(lián)合治療組與其他3組腫瘤細(xì)胞增殖水平的差異。體內(nèi)實(shí)驗(yàn)部分：首先建立裸鼠人前列腺癌皮下移植瘤模型,將其分為4組(每組n=6):(1)對照組；(2)僅射頻加熱組;(3)僅化療組(多西紫杉醇)；(4)射頻加熱-化療聯(lián)合治療組。在治療前、治療后1周及2周用磁共振掃描監(jiān)測各組移植瘤模型的腫瘤體積,最后從病理組織水平分析證實(shí)各組腫瘤組織的凋亡水平。結(jié)果體外實(shí)驗(yàn)部分：在射頻加熱-化療聯(lián)合治療組中,人前列腺癌細(xì)胞增殖水平較其他3組明顯降低(P0.05)；體內(nèi)實(shí)驗(yàn)部分：(1)射頻加熱-化療聯(lián)合治療后小鼠移植瘤體積與其他3組相比明顯縮小(P0.05)；腫瘤組織TUNEL凋亡檢測表明聯(lián)合治療組治療后移植瘤凋亡水平較其他組明顯升高(P0.05)。結(jié)論本實(shí)驗(yàn)從體外和體內(nèi)兩方面驗(yàn)證實(shí)了射頻加熱可以增強(qiáng)前列腺癌局部化療療效,為下一步建立影像引導(dǎo)下,聯(lián)合RFH與腫瘤局部化療兩種治療手段來清除腫瘤消融邊界區(qū)瘤組織這一新的腫瘤介入治療技術(shù)奠定了基礎(chǔ),新型的介入治療方法可能為前列腺癌患者提供新的治療方案。。
[Abstract]:Objective to establish a new interventional therapy for enhancing the efficacy of local chemotherapy for prostate cancer with radiofrequency heating (radiofrequency heat, RFH). Materials and methods this experiment is divided into two parts: in vivo experiment and in vitro experiment. In vitro experiment part: human prostate cancer cell line cultured in vitro was divided into 4 groups: (1) control group; (2) radiofrequency heating group only; (3) only chemotherapy group (docetaxel); (4) combined radiofrequency heating and chemotherapy group. After the cells in each group were treated with different methods, the proliferation level of tumor cells in the radiofrequency heating-chemotherapy combined therapy group was compared with that in the other three groups by MTS colorimetry. In vivo part: the nude mice model of human prostate cancer transplanted subcutaneously was firstly established and divided into 4 groups (n = 6): (1) control group, (2) radiofrequency heating group only, (3) chemotherapy group (docetaxel only), 3) chemotherapy group (docetaxel group), 3) chemotherapy group (docetaxel group). (4) radiofrequency heating combined with chemotherapy group. Before and 1 and 2 weeks after treatment, the tumor volume of the transplanted tumor model was monitored by magnetic resonance imaging (MRI). Finally, the apoptosis level of the tumor tissue was confirmed by pathological analysis. Results in the part of in vitro experiment: the proliferation level of human prostate cancer cells in the radiofrequency heating-chemotherapy group was significantly lower than that in the other three groups (P0.05). In vivo experiment part: (1) after radiofrequency heating-chemotherapy combined treatment, the transplanted tumor volume of mice was significantly smaller than that of the other three groups (P0.05); The apoptotic level of transplanted tumor in the combined treatment group was significantly higher than that in the other groups (P0.05). The TUNEL apoptosis in the tumor tissue was significantly higher than that in the other groups. Conclusion radiofrequency heating can enhance the efficacy of local chemotherapy for prostate cancer in vitro and in vivo. The combination of RFH and local chemotherapy has laid a foundation for the removal of tumor tissue in the tumor ablation boundary region. The new interventional therapy may provide a new therapy for prostate cancer patients.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R737.25

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