【摘要】：目的:分析新疆地區(qū)維、漢族婦女HLA-DRB1等位基因頻率分布情況,探索其與中晚期宮頸癌的相關(guān)聯(lián)系。方法:選取100例中晚期宮頸癌患者(其中維吾爾族患者37例,漢族患者63例)及188例健康對(duì)照(其中維吾爾族健康對(duì)照97例,漢族健康對(duì)照91例),應(yīng)用聚合酶反應(yīng)/序列特異性引物檢測(cè)HLA-DRB1等位基因,根據(jù)不同因素對(duì)基因頻率進(jìn)行比較,并進(jìn)一步了解HLA-DRB1與宮頸癌近期療效及預(yù)后的聯(lián)系。結(jié)果:(1)漢族人群和維吾爾族人群都攜帶有相同的13種HLA-DRB1等位基因;(2)漢族健康對(duì)照組HLA-DRB1*01基因頻率明顯低于維吾爾族健康對(duì)照組,漢族健康對(duì)照組HLA-DRB1*09、HLA-DRB1*12基因頻率明顯高于維吾爾族健康對(duì)照組,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);漢族宮頸癌組HLA-DRB1*07基因頻率明顯低于維族宮頸癌組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。(3)新疆地區(qū)漢族宮頸癌組HLA-DRB1*15基因頻率明顯高于漢族健康對(duì)照組,維吾爾族宮頸癌患者HLA-DRB1*04基因頻率明顯低于維吾爾族健康對(duì)照組,維吾爾族宮頸癌患者HLA-DRB1*07、HLA-DRB1*11基因頻率明顯高于維吾爾族健康對(duì)照組,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。(4)HPV16陽(yáng)性組宮頸癌HLA-DRB1*12基因頻率明顯低于HPV16陰性組,中分化鱗癌組HLA-DRB1*04基因頻率明顯高于非中分化鱗癌組,52歲年齡組宮頸癌HLA-DRB1*11基因頻率明顯低于≥52歲年齡組,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)(5)HLA-DRB1等位基因與宮頸癌近期療效及預(yù)后方面未見(jiàn)明顯聯(lián)系。結(jié)論:(1)漢族人群和維吾爾族人群都攜帶有相同的HLA-DRB1等位基因;(2)維、漢族之間HLA-DRB1等位基因分布存在較大差異性;同一等位基因在不同民族間起著不同作用。(3)HLA-DRB1*15可能是漢族人群宮頸癌的易感基因;HLA-DRB1*07、HLA-DRB1*11可能是維吾爾族人群宮頸癌的易感基因;HLA-DRB1*04可能是維吾爾族人群宮頸癌的保護(hù)基因。(4)攜帶HLA-DRB1*12等位基因的宮頸癌患者可能更不易感染HPV16;攜帶HLA-DRB1*04等位基因的宮頸癌患者可能更易罹患中分化鱗癌;攜帶HLA-DRB1*11等位基因的宮頸癌患者更易在中老年階段發(fā)病。(5)HLA-DRB1等位基因與宮頸癌近期療效及預(yù)后方面未見(jiàn)明顯相關(guān)性。
[Abstract]:Aim: to analyze the distribution of HLA-DRB1 allele frequency in Uygur and Han women in Xinjiang and to explore its association with advanced cervical cancer. Methods: 100 patients with advanced cervical cancer (including 37 Uighur and 63 Han) and 188 healthy controls (97 Uighur and 91 Han) were selected. HLA-DRB1 alleles were detected by polymerase reaction / sequence-specific primers, and the gene frequencies were compared according to different factors, and the relationship between HLA-DRB1 and short-term curative effect and prognosis of cervical cancer was further understood. Results: (1) 13 HLA-DRB1 alleles were found in both Han and Uygur populations. (2) the frequency of HLA-DRB1*01 gene in Han healthy control group was significantly lower than that in Uygur healthy control group, and the HLA-DRB1*09,HLA-DRB1*12 gene frequency in Han healthy control group was significantly higher than that in Uygur healthy control group. The difference was statistically significant (P0.05); The frequency of HLA-DRB1*07 gene in Han cervical cancer group was significantly lower than that in Uygur cervical cancer group (P0.05). (3). The HLA-DRB1*15 gene frequency in Xinjiang Han cervical cancer group was significantly higher than that in Han healthy control group. The frequency of HLA-DRB1*04 gene in Uighur cervical cancer patients was significantly lower than that in Uighur healthy controls, and the HLA-DRB1*07,HLA-DRB1*11 gene frequency in Uighur cervical cancer patients was significantly higher than that in Uighur healthy controls. The HLA-DRB1*12 gene frequency of cervical carcinoma in HPV16 positive group was significantly lower than that in HPV16 negative group, and the HLA-DRB1*04 gene frequency in moderately differentiated squamous cell carcinoma group was significantly higher than that in non-intermediate differentiated squamous cell carcinoma group (P0.05). (4). The frequency of HLA-DRB1*11 gene in 52-year-old group was significantly lower than that in 鈮，

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