【摘要】：目的探討枸杞多糖在白血病患者中的應(yīng)用及對(duì)人前髓細(xì)胞HL-60表面靶細(xì)胞表面相應(yīng)配體(MICA)蛋白表達(dá)水平和自然殺傷(NK)細(xì)胞殺傷活性的影響及機(jī)制。方法取白血病患者HL-60細(xì)胞,隨機(jī)分為觀察1組、觀察2組及觀察3組,分別放入濃度為6、8、10 mg/ml枸杞多糖共同培養(yǎng),未經(jīng)枸杞多糖作用的30例白血病患者HL-60細(xì)胞為對(duì)照組,采用流式細(xì)胞儀測定4組細(xì)胞表面MICA蛋白表達(dá)水平。將4組獲得的溶液與NK細(xì)胞相互混合,20 h后采用四甲基偶氮唑藍(lán)比色(MTT)法檢測NK細(xì)胞對(duì)4組HL-60細(xì)胞的殺傷活性進(jìn)行測定。結(jié)果觀察1組MICA表達(dá)率顯著低于觀察2組、觀察3組(P0.05);觀察1組、觀察2組、觀察3組MICA表達(dá)率高于對(duì)照組(P0.05);隨著靶細(xì)胞、效應(yīng)細(xì)胞比的不斷增加,4組NK細(xì)胞殺傷活性均出現(xiàn)上升趨勢;觀察1組、觀察2組及觀察3組NK細(xì)胞殺傷活性高于對(duì)照組(P0.05);觀察3組NK細(xì)胞殺傷活性,高于觀察1組、觀察2組(P0.05)。結(jié)論白血病患者體內(nèi)HL-60細(xì)胞在枸杞多糖的孵育下能提高細(xì)胞MICA表達(dá)率,且呈濃度正相關(guān)性。同時(shí),枸杞多糖能提高機(jī)體內(nèi)NK細(xì)胞的殺傷活性,能清除微小病灶。
[Abstract]:Aim to investigate the application of Lycium barbarum polysaccharides in patients with leukemia and the effect of Lycium barbarum polysaccharides on the expression of (MICA) protein on the surface of target cells on the surface of human anterior myeloid cells (HL-60) and the killing activity of natural killer (NK) cells and its mechanism. Methods HL-60 cells from leukemia patients were randomly divided into observation group 1, observation group 2 and observation 3 groups. The cells were co-cultured with Lycium barbarum polysaccharides at the concentration of 6,8,10 mg/ml, respectively. The expression of MICA protein on the surface of HL-60 cells from 30 leukemia patients without Lycium barbarum polysaccharide was measured by flow cytometry. The solution obtained from the four groups was mixed with NK cells for 20 hours, and the cytotoxicity of NK cells to four groups of HL-60 cells was determined by (MTT) assay with tetramethylazo blue colorimetric assay. Results the expression rate of MICA in group 1 was significantly lower than that in group 2, and in group 3 (P0.05), the expression rate of MICA in group 1, group 2 and group 3 was higher than that in control group (P0.05). With the increasing ratio of target cells and effector cells, the killing activity of NK cells in four groups increased, and the killing activity of NK cells in group 1, group 2 and group 3 was higher than that in control group (P0.05). The killing activity of NK cells in three groups was higher than that in observation group 1 and group 2 (P0.05). Conclusion HL-60 cells incubated with Lycium barbarum polysaccharide can increase the expression rate of MICA in leukemia patients, and there is a positive correlation between the concentrations. At the same time, Lycium barbarum polysaccharide can improve the killing activity of NK cells and clear the small lesions.
【作者單位】： 廣州中醫(yī)藥大學(xué);廣東醫(yī)科大學(xué);
【基金】：廣東省自然科學(xué)基金項(xiàng)目(No.10451008901001139)
【分類號(hào)】：R733.7

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