【摘要】：[目的]研究10項(xiàng)常見腫瘤標(biāo)志物:癌胚抗原、糖類抗原19-9、糖類抗原72-4、甲胎蛋白、絨毛膜促性腺激素、糖類抗原15-3、糖類抗原125、神經(jīng)元特異性烯醇化酶、細(xì)胞角蛋白19片段和總前列腺抗原的生物學(xué)變異。[方法]依據(jù)美國臨床實(shí)驗(yàn)室標(biāo)準(zhǔn)化研究所相關(guān)評(píng)價(jià)文件對(duì)ROCHE e601電化學(xué)發(fā)光分析儀進(jìn)行校準(zhǔn)及方法學(xué)性能進(jìn)行評(píng)價(jià);通過問卷調(diào)查,選取120位健康成年人建立適合于本實(shí)驗(yàn)室的上述腫瘤標(biāo)志物的參考區(qū)間;并募集55位健康成年人志愿者,采用相關(guān)生物學(xué)變異文獻(xiàn)的實(shí)驗(yàn)方法,研究55例健康成年人志愿者的10項(xiàng)常見腫瘤標(biāo)志物的個(gè)體內(nèi)生物學(xué)變異、個(gè)體間生物學(xué)變異、參考變化值及個(gè)體指數(shù)。[結(jié)果](1)得出了 10項(xiàng)常見腫瘤標(biāo)志物的個(gè)體內(nèi)生物學(xué)變異、個(gè)體間生物學(xué)變異、參考變化值及個(gè)體指數(shù)數(shù)據(jù)。(2)不同檢驗(yàn)項(xiàng)目生物學(xué)變異不同,健康成年人志愿者10項(xiàng)常見腫瘤標(biāo)志物的平均個(gè)體內(nèi)生物學(xué)變異,其中CA15-3、AFP等較小,NSE、CA21-1等較大,其余檢驗(yàn)項(xiàng)目介于兩者之間。(3)健康成年人志愿者10項(xiàng)常見腫瘤標(biāo)志物個(gè)體間生物學(xué)變異,其中CA153較小,CA19-9較大,其余檢驗(yàn)項(xiàng)目介于兩者之間。(4)健康成年人志愿者10項(xiàng)常見腫瘤標(biāo)志物的參考變化值差異較大。(5)健康成年人志愿者各項(xiàng)腫瘤標(biāo)志物的個(gè)體指數(shù)比較接近。[結(jié)論](1)本研究得出10種常見腫瘤標(biāo)志物項(xiàng)目在我國實(shí)驗(yàn)室情況下的基于國人的生物學(xué)變異數(shù)據(jù)庫。(2)建立的基于中國人群的常見腫瘤標(biāo)志物的生物學(xué)變異數(shù)據(jù),與西班牙臨床化學(xué)和分子病理學(xué)協(xié)會(huì)發(fā)布的數(shù)據(jù)庫基本一致,但也有少部分項(xiàng)目存在差異。(3)使用生物學(xué)變異數(shù)據(jù)對(duì)于評(píng)估腫瘤標(biāo)志物的群體參考區(qū)間、參考變化值及臨床診療應(yīng)用有重要價(jià)值。
[Abstract]:[objective] to study 10 common tumor markers: carcinoembryonic antigen, carbohydrate antigen 19-9, carbohydrate antigen 72-4, alpha-fetoprotein, chorionic gonadotropin, carbohydrate antigen 15-3, carbohydrate antigen 125, neuron-specific enolase. Biological variation of cytokeratin 19 fragment and total prostate antigen. [methods] the calibration and methodological performance of ROCHE e601 electroluminescent analyzer were evaluated according to the relevant evaluation documents of American Institute of Clinical Laboratory Standardization. By questionnaire, 120 healthy adults were selected to establish a reference range for the above tumor markers in our laboratory. In addition, 55 healthy adult volunteers were recruited to study the intra-individual biological variation and inter-individual biological variation of 10 common tumor markers in 55 healthy adult volunteers. Reference value and individual index. [results] (1) Intra-individual biological variation, inter-individual biological variation, reference variation value and individual index data of 10 common tumor markers were obtained. (2) the biological variation of different test items was different. The average in vivo biological variation of 10 common tumor markers in healthy adult volunteers was CA15-3,AFP and NSE,CA21-1. The other test items were between the two. (3) the biological variation of 10 common tumor markers in healthy adult volunteers, in which CA153 was small, CA19-9 was larger, The other test items were between the two. (4) the reference values of 10 common tumor markers in healthy adult volunteers were significantly different. (5) the individual index of each tumor marker in healthy adult volunteers was similar. [conclusion] (1) in this study, we obtained the biological variation database of 10 common tumor markers in Chinese laboratory. (2) the biological variation data of common tumor markers based on Chinese population were established. It is basically consistent with the database published by the Spanish Association of Clinical Chemistry and Molecular Pathology, but there are also differences in a small number of items. (3) the use of biological variation data to evaluate the population reference interval for tumor markers. Reference value and clinical application have important value.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R730.43

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 Wanqing Chen;Rongshou Zheng;Siwei Zhang;Hongmei Zeng;Tingting Zuo;Changfa Xia;Zhixun Yang;Jie He;;Cancer incidence and mortality in China in 2013:an analysis based on urbanization level[J];Chinese Journal of Cancer Research;2017年01期

2 魏紅璐;王萍;;基于WS/T 405-2012行標(biāo)的血細(xì)胞分析參考區(qū)間驗(yàn)證[J];國際檢驗(yàn)醫(yī)學(xué)雜志;2016年05期

3 張學(xué)東;李曉華;李建華;王新杰;侯志偉;楊帆;;原發(fā)性肝癌血清腫瘤標(biāo)志物的研究進(jìn)展[J];承德醫(yī)學(xué)院學(xué)報(bào);2014年06期

4 文軍;;原發(fā)性肝癌血清腫瘤標(biāo)記物的研究進(jìn)展[J];中國傷殘醫(yī)學(xué);2013年09期

5 沈立松;周韻斕;尚紅;潘柏申;;腫瘤標(biāo)志物的臨床應(yīng)用建議[J];中華檢驗(yàn)醫(yī)學(xué)雜志;2012年02期

6 麥愛芬;;原發(fā)性肝癌血清腫瘤標(biāo)記物的研究進(jìn)展[J];檢驗(yàn)醫(yī)學(xué)與臨床;2011年23期

7 趙旭;李安慶;于剛;王林忠;;原發(fā)性肝癌的血清腫瘤標(biāo)記物研究[J];肝膽外科雜志;2011年04期

8 陽蘋;張莉萍;李武縣;羅疏薇;陳宏礎(chǔ);;6σ質(zhì)量管理理論在電化學(xué)發(fā)光法測定腫瘤標(biāo)志物中的應(yīng)用[J];重慶醫(yī)學(xué);2010年24期

9 曾潔;趙海艦;張傳寶;申子瑜;陳文祥;;19項(xiàng)臨床生化檢驗(yàn)項(xiàng)目的分析前變異和個(gè)體內(nèi)生物學(xué)變異[J];中華檢驗(yàn)醫(yī)學(xué)雜志;2010年08期

10 王鈺;;原發(fā)性肝癌血清腫瘤標(biāo)記物檢測研究進(jìn)展[J];吉林醫(yī)學(xué);2010年07期

，

本文編號(hào)：2339327