【摘要】：本研究分別從細(xì)胞層面和在體層面,研究納米氧化石墨烯(nano graphene oxide,NGO)聯(lián)合805 nm近紅外激光對于腫瘤的殺傷和免疫刺激作用.細(xì)胞實(shí)驗(yàn)證實(shí)NGO具有良好的光熱轉(zhuǎn)換效應(yīng),聯(lián)合激光治療,能夠有效殺傷腫瘤細(xì)胞.此外,NGO能夠刺激巨噬細(xì)胞產(chǎn)生IL-6及TNFα,并增強(qiáng)激光殺傷的腫瘤細(xì)胞對巨噬細(xì)胞的免疫刺激效應(yīng).在體實(shí)驗(yàn)中,在小鼠背部不同位置使用同一瘤株種植兩個(gè)瘤塊,模擬局部原發(fā)腫瘤和轉(zhuǎn)移瘤.實(shí)驗(yàn)結(jié)果表明,NGO聯(lián)合激光治療可以有效消融局部腫瘤,并且,未治療的遠(yuǎn)隔部位腫瘤生長速度也顯著降低.說明NGO聯(lián)合激光治療的方式在局部殺傷原位腫瘤的同時(shí),可能誘發(fā)了機(jī)體的抗腫瘤免疫反應(yīng),從而達(dá)到了抑制遠(yuǎn)隔腫瘤生長的效果.
[Abstract]:In this study, the cytotoxic and immunostimulatory effects of nano-graphene oxide (nano graphene oxide,NGO) combined with 805 nm near infrared laser on tumor were studied at the cellular and in vivo levels, respectively. Cell experiments have proved that NGO has a good photothermal conversion effect, combined with laser therapy, can effectively kill tumor cells. In addition, NGO stimulated macrophages to produce IL-6 and TNF 偽, and enhanced the immunostimulatory effect of laser killed tumor cells on macrophages. In vivo, two tumor blocks were implanted with the same tumor strain at different sites in the back of mice to simulate local primary tumor and metastatic tumor. The experimental results show that NGO combined with laser therapy can effectively ablation local tumors, and the growth rate of untreated distant tumors is also significantly reduced. The results suggest that the combination of NGO and laser therapy may induce antitumor immune response and inhibit the growth of distant tumor.
【作者單位】： 天津醫(yī)科大學(xué)腫瘤醫(yī)院 介入腫瘤治療科 天津市腫瘤防治重點(diǎn)實(shí)驗(yàn)室;Biophotonics
【基金】：美國國家衛(wèi)生研究院(RS20132225-106,國家研究資源中心的INBRE項(xiàng)目) 俄克拉荷馬州科學(xué)技術(shù)促進(jìn)中心(HR16-085)資助項(xiàng)目~~
【分類號】：R737.9

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