【摘要】：檢測T細胞免疫球蛋白及黏蛋白分子3(T cell immunoglobulin and mucin domain containing molecules 3,TIM-3)在胃癌患者固有免疫細胞(γδT細胞與NK細胞)中的表達率,探討其與臨床病理特征及術(shù)后中位無進展生存期(progress free survival,PFS)的關(guān)系。收集臨床病理資料齊全的胃癌患者26例,經(jīng)流式細胞技術(shù)檢測TIM-3在胃癌組織、癌旁組織及外周血γδT細胞、NK細胞中的表達率,并分析其與臨床病理因素及中位PFS的相關(guān)性。結(jié)果發(fā)現(xiàn),TIM-3在胃癌組織和癌旁組織中γδT細胞上均表達,而2類組織中γδT細胞上TIM-3的表達率差異無統(tǒng)計學意義(P=0.074),但均顯著高于其在外周血γδT細胞上的表達水平(P0.001;P0.001)。TIM-3在外周血NK細胞中表達率較高,且TIM-3在外周血中NK細胞表達率顯著高于胃癌組織及癌旁組織(P0.001;P0.001),而癌組織與癌旁組織之間差異無統(tǒng)計學意義(P=0.097)。NK細胞中TIM-3的表達率分別與腫瘤浸潤深度和TNM分期相關(guān)(P0.01),γδT細胞中TIM-3表達率與TNM分期有關(guān)(P0.01)。γδT細胞及NK細胞中TIM-3表達率較低的胃癌患者比TIM-3表達率較高的胃癌患者的中位PFS更長(P=0.014;P=0.028),提示TIM-3在胃癌的進展過程中起到重要作用。
[Abstract]:The expression rate of T cell immunoglobulin and mucin molecule 3 (T cell immunoglobulin and mucin domain containing molecules 3 TIM-3 in innate immune cells (緯 未 T cells and NK cells) of patients with gastric cancer was detected. To investigate its relationship with clinicopathological features and median progressive survival time (progress free survival,PFS). 26 cases of gastric cancer with complete clinicopathological data were collected. The expression rate of TIM-3 in gastric cancer tissues, peripheral blood 緯 未 T cells and NK cells was detected by flow cytometry, and the correlation with clinicopathological factors and median PFS was analyzed. The results showed that TIM-3 was expressed on 緯 未 T cells in gastric cancer and paracancerous tissues, but there was no significant difference in the expression rate of TIM-3 on 緯 未 T cells between the two types of tissues (P0. 074). The expression level of 緯 未 T cells was significantly higher than that of 緯 未 T cells (P0.001). The expression rate of TIM-3 in peripheral blood NK cells was higher than that in gastric cancer tissues and adjacent tissues (P0.001), and the expression rate of TIM-3 in peripheral blood NK cells was significantly higher than that in gastric cancer tissues and adjacent tissues (P0.001). There was no significant difference between cancer tissue and paracancerous tissue (P0. 097). NK cell TIM-3 expression was correlated with the depth of tumor invasion and TNM stage (P0. 01), respectively), but there was no significant difference between P0. 001 and paracancerous tissues (P0. 097). The expression rate of TIM-3 in 緯 未 T cells was related to the TNM stage (P0.01). The median PFS of patients with lower TIM-3 expression in 緯 未 T cells and NK cells was longer than that in patients with higher TIM-3 expression rate (P0.014). It suggests that TIM-3 plays an important role in the progression of gastric cancer.
【作者單位】： 蘇州大學附屬第三醫(yī)院腫瘤科;江蘇省腫瘤免疫治療工程技術(shù)研究中心;
【基金】：國家科技支撐項目(2015BAI12B12) 國家自然科學基金資助項目(31570877;31570908;81171653;30950022);國家自然科學基金海外及港澳學者合作研究基金資助項目(31428005) 江蘇省重點研發(fā)計劃(BE2015634) 常州市衛(wèi)生系領(lǐng)軍人才計劃項目(2016ZCLJ022)
【分類號】：R735.2

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