肺癌、腸癌多腫瘤標志物聯(lián)合檢測及菜菔硫烷預防化學性致癌的效果初探
發(fā)布時間：2018-09-16 20:27
【摘要】：目的：研究巨噬細胞抑制因子-1(MIC-1)在較大樣本的早期肺癌和肺癌中的輔助診斷價值,并評價多腫瘤標志物聯(lián)合應用相關(guān)數(shù)據(jù)的臨床意義。方法：應用本實驗室自主研制的MIC-1定量檢測試劑盒及Roche Cobas 601電化學發(fā)光免疫分析儀分別檢測663例不同臨床分期的未治療肺癌患者和488例正常人群血清樣本中的MIC-1、CEA、CA125、NSE、SCC和Cyfra21-1水平和分布,分析患者血清MIC-1水平與肺癌臨床分期、病理分型和細胞分化程度的關(guān)系,并研究多標志聯(lián)合檢測的價值。結(jié)果：肺癌患者血清MIC-1水平顯著高于正常人群(p0.001)；MIC-1水平隨臨床分期顯著上升(p0.001),且與腫瘤浸潤(p0.001)、淋巴結(jié)轉(zhuǎn)移(p=0.037)、遠端轉(zhuǎn)移(p0.001)和腫瘤分化程度(p0.001)顯著相關(guān)。MIC-1肺癌診斷的敏感性高于其它五種肺癌標志物的聯(lián)合應用(76.6% vs 72.2%),在腺癌診斷中更為突出(腺癌：74.7% vs68.9%；鱗癌：81.6% vs 82.8%；小細胞癌：84.9% vs 83.0%)。尤為顯著的是,MIC-1診斷Ⅰ期和ⅡI期肺癌的敏感性可達70.2%和69.9%,顯著優(yōu)于五種常用標志物中敏感性最高的Cyfra21-1(Ⅰ期20.7%,Ⅱ期52.1%)；MIC-1聯(lián)合上述五種標志物診斷Ⅰ期和Ⅱ期肺癌的靈敏度可達79.8%和87.7%。結(jié)論：MIC-1是肺癌尤其是早期肺癌有價值的血清腫瘤標志物,MIC-1和CEA、 CA125、NSE、SCC、Cyfra21-1聯(lián)合檢測用于普通人群體檢和肺癌早期診斷具有重要的臨床意義和價值。目的：研究巨噬細胞抑制因子-1(MIC-1)在較大樣本的早期腸癌和腸癌中的輔助診斷價值,并評價多腫瘤標志物聯(lián)合應用相關(guān)數(shù)據(jù)的臨床意義。方法：應用本實驗室自主研制的MIC-1定量檢測試劑盒及Roche Cobas 601電化學發(fā)光免疫分析儀分別檢測441例不同臨床分期的未治療腸癌患者、179例治療患者和617例正常人群血清樣本中的MIC-1、CA19-9和CEA水平和分布,分析患者血清MIC-1水平與腸癌臨床分期、病理分型和細胞分化程度的關(guān)系,并研究多標志物聯(lián)合檢測的價值。結(jié)果：腸癌患者血清MIC-1水平均極顯著高于正常人群(p0.001),腸癌水平隨臨床分期進展而顯著上升(p0.001),與腫瘤浸潤(p=0.01)、淋巴結(jié)轉(zhuǎn)移(p=0.017)、遠端轉(zhuǎn)移(p0.001)及發(fā)病部位(p0.05)有關(guān)。MIC-1的診斷敏感性高于CA19-9和CEA(52.4% vs 14.5%、36.3%),尤其在早期診斷時具有更高的敏感性(Ⅰ期43.4%,Ⅱ期55.0%),而MIC-1、CA19-9、CEA聯(lián)合診斷時敏感性高達69.6%,Ⅰ期腸癌診斷敏感性高達52.8%,Ⅱ期腸癌診斷敏感性高達70.7%。結(jié)論：MIC-1是腸癌尤其是早期腸癌有價值的血清腫瘤標志物,MIC-1和CA19-9、CEA聯(lián)合檢測用于普通人群體檢和腸癌早期診斷具有重要的臨床意義和價值。目的：探討萊菔硫烷在小鼠3,4-苯并芘誘導前胃癌過程中的化學預防作用。方法：小鼠以10g/L 3,4-苯并芘灌胃誘導前胃癌,實驗組小鼠從第一次灌胃前2周持續(xù)到第四次灌胃后2周的時間內(nèi)飼喂不同濃度的萊菔硫烷特殊小鼠飼料,分別為每千克飼料500mg、250mg萊菔硫烷,第四次灌胃后每隔5周處死一批小鼠,對前胃組織進行病理組織學觀察。結(jié)果：小鼠飼喂第16周前胃組織出現(xiàn)腫瘤,且隨時間增長,腫瘤數(shù)量增多,直徑增大。不同濃度萊菔硫烷對小鼠前胃腫瘤出現(xiàn)有抑制作用,并呈現(xiàn)劑量依賴效應。第26周時,高劑量萊菔硫烷對小鼠平均荷瘤數(shù)的抑制率為30.7%,低劑量萊菔硫烷抑制率為18.2%。HE染色切片顯示26周時,高劑量萊菔硫烷對小鼠3,4-苯并芘誘導出現(xiàn)增生性病變、癌前病變、原位癌和浸潤癌的抑制率分別為14.0%、28.1%、56.1%和100%,低劑量組抑制率分別為7.4%、29.1%、34.1%和16.7%。結(jié)論：萊菔硫烷能夠抑制小鼠3,4-苯并芘誘導小鼠前胃癌出現(xiàn)過程中增生性病變、癌前病變、原位癌和浸潤癌的病灶數(shù),對小鼠前胃癌的誘發(fā)有明確的預防效果。
[Abstract]:Objective: To study the diagnostic value of macrophage inhibitory factor-1 (MIC-1) in large sample of early lung cancer and lung cancer, and to evaluate the clinical significance of combined application of multiple tumor markers. The levels and distribution of MIC-1, CEA, CA125, NSE, SCC and Cyfra21-1 in serum samples of 663 patients with untreated lung cancer at different clinical stages and 488 normal persons were detected, and the relationship between the levels of MIC-1 and clinical stage, pathological classification and cell differentiation of lung cancer was analyzed. The serum level of MIC-1 was significantly higher than that of the normal population (p0.001); the level of MIC-1 increased significantly with clinical stage (p0.001), and was significantly correlated with tumor infiltration (p0.001), lymph node metastasis (p = 0.037), distal metastasis (p0.001) and tumor differentiation (p0.001). The diagnostic sensitivity of MIC-1 lung cancer was higher than that of other five lung cancer markers (76.6%). Vs 72.2% (adenocarcinoma: 74.7% vs 68.9%; squamous carcinoma: 81.6% vs 82.8%; small cell carcinoma: 84.9% vs 83.0%). Conclusion: MIC-1 is a valuable serum tumor marker for lung cancer, especially for early stage lung cancer. The combined detection of MIC-1 and CEA, CA125, NSE, SCC, Cyfra21-1 has important clinical significance and value in the physical examination of general population and early diagnosis of lung cancer. Objective: To study the diagnostic value of macrophage inhibitory factor-1 (MIC-1) in large samples of early colorectal cancer and colorectal cancer, and to evaluate the clinical significance of combined application of multiple tumor markers and related data. The serum levels of MIC-1, CA19-9 and CEA were measured in 441 untreated, 179 untreated and 617 normal subjects. The relationship between serum levels of MIC-1 and clinical stage, pathological classification and cell differentiation of colorectal cancer was analyzed. The serum level of MIC-1 in cancer patients was significantly higher than that in normal people (p0.001). The level of intestinal cancer increased significantly with clinical stage (p0.001). It was associated with tumor invasion (p = 0.01), lymph node metastasis (p = 0.017), distal metastasis (p0.001) and site of disease (p0.05). The diagnostic sensitivity of MIC-1 was higher than that of CA19-9 and CEA (52.4% vs 14.5%, 36.3%) especially in early stage. The sensitivity of combined diagnosis of MIC-1, CA19-9 and CEA was 69.6%, 52.8% for stage I and 70.7% for stage II. Conclusion: MIC-1 is a valuable serum tumor marker for colorectal cancer, especially for early stage colorectal cancer. Objective: To investigate the chemopreventive effect of sulforaphane on 3,4-benzopyrene-induced precancerous carcinoma in mice. Methods: The precancerous carcinoma was induced by intragastric administration of 10 g/L 3,4-benzopyrene in mice, and the precancerous carcinoma lasted from 2 weeks before the first gastric administration to 4 times in the experimental group. The mice were fed with different concentrations of sulforaphane (500 mg/kg, 250 mg/kg) for 2 weeks after gastric administration. A batch of mice were sacrificed every 5 weeks after the fourth gastric administration. The histopathological changes of the forestomach tissues were observed. At the 26th week, the inhibition rate of high dose of sulforaphane was 30.7% and that of low dose of sulforaphane was 18.2%. HE staining showed that at the 26th week, high dose of sulforaphane inhibited 3,4-in mice. The inhibition rates of benzopyrene-induced proliferative lesions, precancerous lesions, carcinoma in situ and invasive carcinoma were 14.0%, 28.1%, 56.1% and 100%, respectively. The inhibition rates of low dose group were 7.4%, 29.1%, 34.1% and 16.7% respectively. Conclusion: Raphane can inhibit the proliferative lesions, precancerous lesions and carcinoma in situ in mice with 3,4-benzopyrene-induced precancerous lesions. And the number of infiltrating cancer has a definite preventive effect on the induction of gastric cancer in mice.
【學位授予單位】：北京協(xié)和醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R730
本文編號：2244703
[Abstract]:Objective: To study the diagnostic value of macrophage inhibitory factor-1 (MIC-1) in large sample of early lung cancer and lung cancer, and to evaluate the clinical significance of combined application of multiple tumor markers. The levels and distribution of MIC-1, CEA, CA125, NSE, SCC and Cyfra21-1 in serum samples of 663 patients with untreated lung cancer at different clinical stages and 488 normal persons were detected, and the relationship between the levels of MIC-1 and clinical stage, pathological classification and cell differentiation of lung cancer was analyzed. The serum level of MIC-1 was significantly higher than that of the normal population (p0.001); the level of MIC-1 increased significantly with clinical stage (p0.001), and was significantly correlated with tumor infiltration (p0.001), lymph node metastasis (p = 0.037), distal metastasis (p0.001) and tumor differentiation (p0.001). The diagnostic sensitivity of MIC-1 lung cancer was higher than that of other five lung cancer markers (76.6%). Vs 72.2% (adenocarcinoma: 74.7% vs 68.9%; squamous carcinoma: 81.6% vs 82.8%; small cell carcinoma: 84.9% vs 83.0%). Conclusion: MIC-1 is a valuable serum tumor marker for lung cancer, especially for early stage lung cancer. The combined detection of MIC-1 and CEA, CA125, NSE, SCC, Cyfra21-1 has important clinical significance and value in the physical examination of general population and early diagnosis of lung cancer. Objective: To study the diagnostic value of macrophage inhibitory factor-1 (MIC-1) in large samples of early colorectal cancer and colorectal cancer, and to evaluate the clinical significance of combined application of multiple tumor markers and related data. The serum levels of MIC-1, CA19-9 and CEA were measured in 441 untreated, 179 untreated and 617 normal subjects. The relationship between serum levels of MIC-1 and clinical stage, pathological classification and cell differentiation of colorectal cancer was analyzed. The serum level of MIC-1 in cancer patients was significantly higher than that in normal people (p0.001). The level of intestinal cancer increased significantly with clinical stage (p0.001). It was associated with tumor invasion (p = 0.01), lymph node metastasis (p = 0.017), distal metastasis (p0.001) and site of disease (p0.05). The diagnostic sensitivity of MIC-1 was higher than that of CA19-9 and CEA (52.4% vs 14.5%, 36.3%) especially in early stage. The sensitivity of combined diagnosis of MIC-1, CA19-9 and CEA was 69.6%, 52.8% for stage I and 70.7% for stage II. Conclusion: MIC-1 is a valuable serum tumor marker for colorectal cancer, especially for early stage colorectal cancer. Objective: To investigate the chemopreventive effect of sulforaphane on 3,4-benzopyrene-induced precancerous carcinoma in mice. Methods: The precancerous carcinoma was induced by intragastric administration of 10 g/L 3,4-benzopyrene in mice, and the precancerous carcinoma lasted from 2 weeks before the first gastric administration to 4 times in the experimental group. The mice were fed with different concentrations of sulforaphane (500 mg/kg, 250 mg/kg) for 2 weeks after gastric administration. A batch of mice were sacrificed every 5 weeks after the fourth gastric administration. The histopathological changes of the forestomach tissues were observed. At the 26th week, the inhibition rate of high dose of sulforaphane was 30.7% and that of low dose of sulforaphane was 18.2%. HE staining showed that at the 26th week, high dose of sulforaphane inhibited 3,4-in mice. The inhibition rates of benzopyrene-induced proliferative lesions, precancerous lesions, carcinoma in situ and invasive carcinoma were 14.0%, 28.1%, 56.1% and 100%, respectively. The inhibition rates of low dose group were 7.4%, 29.1%, 34.1% and 16.7% respectively. Conclusion: Raphane can inhibit the proliferative lesions, precancerous lesions and carcinoma in situ in mice with 3,4-benzopyrene-induced precancerous lesions. And the number of infiltrating cancer has a definite preventive effect on the induction of gastric cancer in mice.
【學位授予單位】：北京協(xié)和醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R730
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