【摘要】：目的·探討褐藻糖膠對(duì)多發(fā)性骨髓瘤U266細(xì)胞自噬、遷移及侵襲的影響。方法·褐藻糖膠處理細(xì)胞后,透射電子顯微鏡(TEM)觀察細(xì)胞自噬小體,Transwell小室觀察細(xì)胞遷移及侵襲能力,Western blotting測(cè)定LC3-Ⅱ/LC3-Ⅰ、Beclin-1、P62、MMP9、CXCR4、p-AKT/T-AKT、p-m TOR/T-m TOR蛋白表達(dá),ELISA法檢測(cè)細(xì)胞培養(yǎng)液中MMP9含量。結(jié)果·(1)TEM觀察發(fā)現(xiàn),褐藻糖膠處理組自噬小體增加。(2)褐藻糖膠可降低U266細(xì)胞遷移及侵襲能力,并呈現(xiàn)出劑量依賴(lài)性,氯喹可阻斷該抑制作用。(3)Western blotting結(jié)果顯示,LC3-Ⅱ/LC3-Ⅰ、Beclin-1及MMP9蛋白表達(dá)隨褐藻糖膠濃度增加而增加,P62、CXCR4、p-AKT/T-AKT及p-m TOR/T-m TOR蛋白表達(dá)隨褐藻糖膠濃度增加而降低。(4)ELISA結(jié)果顯示,褐藻糖膠處理組細(xì)胞培養(yǎng)液中MMP9的含量下降。結(jié)論·褐藻糖膠可誘導(dǎo)多發(fā)性骨髓瘤U266細(xì)胞自噬,抑制該細(xì)胞的遷移、侵襲能力。
[Abstract]:Objective to investigate the effects of fucoidan on autophagy, migration and invasion of multiple myeloma cell line U266. Methods after the cells were treated with fucoidan, the cell migration and invasiveness were observed by transwell chamber of autophagy body by transmission electron microscopy (TEM). The expression of MMP9 in cell culture medium was detected by LC3- 鈪，

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