【摘要】：目的:探究MAT1A、MAT2A、SAMe在肝內(nèi)膽管癌組織中的表達(dá)情況和意義;方法:搜集2012.1.26至2016.3.29于蕪湖市第二人民醫(yī)院及弋磯山醫(yī)院行肝葉部分切除術(shù),術(shù)后病理確診為肝內(nèi)膽管癌的60例病例資料和肝內(nèi)膽管結(jié)石的86例病例資料為研究對(duì)象,病例資料包括臨床病理資料及標(biāo)本蠟塊,分別為觀察組和對(duì)照。應(yīng)用組織芯片和免疫組織化學(xué)技術(shù)比較MAT1A、MAT2A、SAMe在觀察組和對(duì)照組中的表達(dá)情況;分析不同物質(zhì)表達(dá)之間的相關(guān)性;分析肝內(nèi)膽管癌臨床病理因素與物質(zhì)表達(dá)的關(guān)系;并對(duì)觀察組的病人進(jìn)行電話隨訪,統(tǒng)計(jì)術(shù)后生存時(shí)間;結(jié)果:觀察組中MAT1A、MAT2A蛋白的表達(dá)明顯較對(duì)照組弱(P0.05),SAMe蛋白在觀察組和對(duì)照組中的含量均較低,但觀察組低于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05);MAT1A、MAT2A、SAMe在肝內(nèi)膽管癌組織中的表達(dá)兩兩之間呈正相關(guān)關(guān)系(P0.01);有膽道探查史的患者ICC組織中的MAT1A表達(dá)增多(P0.05),不同臨床分期的肝內(nèi)膽管癌組織中的MAT2A的表達(dá)不同(P0.05),不同分化程度的ICC組織中SAMe的含量具有差異性(P0.05);肝內(nèi)膽管癌患者術(shù)后中位生存期:13月,術(shù)后1年生存率53.10%,2年生存率31.80%;結(jié)論:在肝內(nèi)膽管癌的組織中MAT1A、MAT2A表達(dá)降低,SAMe的含量也較正常組織減少,以上指標(biāo)的變化在肝內(nèi)膽管上皮細(xì)胞的癌變過(guò)程中可能具有重要意義。
[Abstract]:Objective: to investigate the expression and significance of MAT1A- mati2An2A- SAMe in intrahepatic cholangiocarcinoma. Methods: partial liver lobectomy was performed in Wuhu second people's Hospital and Yiji Mountain Hospital from January 26 to March 29, 2016.Methods: the patients were divided into two groups: the second people's Hospital of Wuhu City and the second people's Hospital of Wuhu City. The data of 60 cases of intrahepatic cholangiocarcinoma and 86 cases of intrahepatic cholelithiasis were studied. Tissue microarray and immunohistochemical technique were used to compare the expression of MAT1Am2AMA-SAMe in observation group and control group, to analyze the correlation between the expression of different substances, to analyze the relationship between clinicopathological factors and substance expression in intrahepatic cholangiocarcinoma. The patients in the observation group were followed up by telephone to calculate the postoperative survival time. Results: the expression of MAT1Anm2A protein in the observation group was significantly lower than that in the control group (P0.05), but the content of SAMe protein in the observation group was lower than that in the control group, but it was lower in the observation group than in the control group. The difference was statistically significant (P0.05). The positive correlation was found between the expression of MAT2A in intrahepatic cholangiocarcinoma tissues (P0.01), the expression of MAT1A in ICC tissues of patients with history of exploration of biliary tract was increased (P0.05), and the expression of MAT2A in intrahepatic cholangiocarcinoma tissues with different clinical stages was significantly higher than that in patients with biliary tract exploration history (P0.01). The expression of SAMe was different (P0.05) and the content of SAMe was different in ICC tissues with different differentiation (P0.05). The median survival time of patients with intrahepatic cholangiocarcinoma was 13 months. The 1 year survival rate was 53.10%, and the 2 year survival rate was 31.80%. Conclusion: the expression of MAT1Anm2A in intrahepatic cholangiocarcinoma is lower than that in normal tissue. The changes of above indexes may play an important role in the carcinogenesis of intrahepatic bile duct epithelial cells.
【學(xué)位授予單位】：皖南醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.8

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