【摘要】：目的了解幽門螺桿菌CagA上調(diào)CIP2A表達(dá)的分子機(jī)制。方法培養(yǎng)AGS細(xì)胞和GES-1細(xì)胞,一部分細(xì)胞分別轉(zhuǎn)染CagA陽(yáng)性質(zhì)粒WT-cagA和陰性對(duì)照質(zhì)粒pcDNA3.1;另一部分細(xì)胞用B-Raf和JNK2信號(hào)抑制劑作用,然后分別轉(zhuǎn)染質(zhì)粒WT-cagA和質(zhì)粒pcDNA3.1,Western blot檢測(cè)CIP2A表達(dá),細(xì)胞克隆形成試驗(yàn)檢測(cè)細(xì)胞增殖能力,細(xì)胞遷移試驗(yàn)檢測(cè)細(xì)胞遷移能力結(jié)果 AGS細(xì)胞和GES-1細(xì)胞轉(zhuǎn)染CagA陽(yáng)性質(zhì)粒WT-cagA后CIP2A表達(dá)量增多;細(xì)胞經(jīng)B-Raf和JNK2的信號(hào)抑制劑作用后轉(zhuǎn)染CagA陽(yáng)性質(zhì)粒WT-cagA,其CIP2A表達(dá)量比僅轉(zhuǎn)染質(zhì)粒WT-cagA的細(xì)胞表達(dá)量少,細(xì)胞的克隆數(shù)減少,細(xì)胞遷移能力降低。結(jié)論幽門螺桿菌CagA進(jìn)入細(xì)胞通過(guò)B-Raf和JNK2及其參與的信號(hào)途徑調(diào)節(jié)原癌蛋白CIP2A的表達(dá),進(jìn)而影響細(xì)胞的克隆形成能力和遷移能力。
[Abstract]:Objective to investigate the molecular mechanism of CagA upregulation of CIP2A expression in Helicobacter pylori. Methods AGS cells and GES-1 cells were cultured, some of them were transfected with CagA positive plasmid WT-cagA and the other were transfected with B-Raf and JNK2 signal inhibitors, and then transfected with WT-cagA and pcDNA3.1 Western blot to detect the expression of CIP2A. The ability of cell proliferation was detected by cell clone formation assay, and the expression of CIP2A was increased after transfection of CagA positive plasmid WT-cagA into AGS cells and GES-1 cells by cell migration assay. After transfection of WT-cagA into CagA positive plasmid WT-cagA by signal inhibitor of B-Raf and JNK2, the expression of CIP2A was less than that of only transfected plasmid WT-cagA, the number of cell clones was decreased, and the ability of cell migration was decreased. Conclusion Helicobacter pylori CagA enters the cells and regulates the expression of proto-oncoprotein CIP2A through B-Raf, JNK2 and its signal pathway, which affects the ability of cell clone formation and migration.
【作者單位】： 鄭州大學(xué)第一附屬醫(yī)院麻醉科;泰山醫(yī)學(xué)院病原生物學(xué)教研室;泰山醫(yī)學(xué)院附屬醫(yī)院檢驗(yàn)科;泰山醫(yī)學(xué)院公共衛(wèi)生學(xué)院;山東省泰山療養(yǎng)院內(nèi)科;泰山醫(yī)學(xué)院第二臨床醫(yī)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.81602455) 山東省自然科學(xué)基金項(xiàng)目(No.ZR2013HM033,ZR2013HL057,ZR2013HL060) 泰山醫(yī)學(xué)院重大科研專項(xiàng)(No.2014GCC10) 泰安市科研課題(No.2015NS2098,2016NS1074,2016NS1089)
【分類號(hào)】：R735.2

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