【摘要】：目的探討局部高脂肪酸和高碳酸對HCC827人肺腺癌移植瘤鈣化及生長的影響。方法選取40只直徑在10~15 mm之間的HCC827人肺腺癌移植瘤裸鼠模型,隨機分為4組,對照組,油酸組,碳酸氫鈉(NaHCO_3)組,油酸+NaHCO_3組,每組10只,分別瘤內(nèi)注射0.9%NaCl 0.2 ml,油酸0.2 ml,NaHCO_3(595 mmol/L)0.2 ml,油酸0.1 ml+NaHCO_3(595 mmol/L)0.1 ml,觀察腫瘤生長的體積大小及腫瘤組織鈣鹽沉積情況,結(jié)果用SPSS 17.0統(tǒng)計軟件對腫瘤體積變化進行分析,腫瘤體積大小用均數(shù)±標準差表示,多組間比較采用方差分析,各組間兩兩比較采用LSD-t檢驗。結(jié)果各組移植瘤注藥后體積從小到大依次是油酸組、油酸+NaHCO_3組、NaHCO_3組和對照組,組間差異有統(tǒng)計學意義(F=5.773,P=0.0020.01);注藥后體積差值組間差異有統(tǒng)計學意義(F=7.242,P=0.0010.01)。由于分組誤差,所以各組在注藥前體積大小不一,但差異無統(tǒng)計學意義(P0.05)。各組間兩兩比較的LSD-t檢驗結(jié)果顯示,與對照組比較,油酸組、NaHCO_3組、油酸+NaHCO_3組注藥后體積及體積差值差異均有統(tǒng)計學意義(注藥后體積:P分別為0.001、0.006、0.002,體積差值:P分別為0、0.047、0.002);與油酸組比較,NaHCO_3組、油酸+NaHCO_3組注藥后體積及體積差值差異均有統(tǒng)計學意義(注藥后體積:P分別為0.420、0.742,體積差值:P分別為0.023、0.275);與NaHCO_3組比較,油酸+NaHCO_3組注藥后體積及體積差值差異均有統(tǒng)計學意義(注藥后體積:P為0.631,體積差值:P為0.212)。HE染色結(jié)果顯示:油酸組和油酸+NaHCO_3組明顯有鈣鹽沉積,NaHCO_3組和對照組未見明顯鈣鹽沉積。結(jié)論局部高油酸可促進腫瘤鈣鹽沉積,油酸可抑制腫瘤的生長;局部高NaHCO_3可抑制腫瘤生長,但停用NaHCO_3后腫瘤體積又會繼續(xù)增長,可能與NaHCO_3改變了腫瘤的偏酸性環(huán)境有關(guān),并且油酸和NaHCO_3有協(xié)同抑制腫瘤生長的趨勢。
[Abstract]:Objective to investigate the effects of local high fatty acid and high carbonic acid on calcification and growth of transplanted lung adenocarcinoma in HCC827. Methods Forty nude mice with a diameter of 10 ~ 15 mm were randomly divided into 4 groups: control group, oleic acid group, sodium bicarbonate (NaHCO_3) group and oleic acid NaHCO_3 group. 0.9%NaCl 0.2 ml, oleic acid 0.2 ml (595 mmol/L) 0.2 ml and oleic acid 0.1 ml NaHCO_3 (595 mmol/L) 0.1 ml were injected into the tumor respectively. The volume of tumor growth and calcium deposition in tumor tissue were observed. Results the changes of tumor volume were analyzed by SPSS 17.0 statistical software. The volume of tumor was expressed by mean 鹵standard deviation. The analysis of variance was used to compare the tumor volume among groups and the LSD-t test was used to compare the tumor volume between two groups. Results the volume of transplanted tumor after injection was oleic acid group, oleic acid NaHCO_3 group and control group, the difference between groups was statistically significant (F5. 773, P < 0. 0020.01), and the difference of volume after injection was statistically significant (FG. 7. 242, P 0. 0010. 01). Because of the grouping error, the volume of each group was different before injection, but the difference was not statistically significant (P0.05). The results of LSD-t test showed that compared with the control group, oleic acid group was treated with NaHCO _ 3 group. There were significant differences in volume and volume difference between oleic acid NaHCO_3 group and oleic acid NaHCO_3 group. There were significant differences in volume and volume difference between NaHCO_3 group and NaHCO_3 group (volume: P was 0.420 鹵0.742and volume difference was 0.023 / 0.275, respectively), compared with NaHCO_3 group, the difference of volume and volume of oleic acid NaHCO_3 group was statistically significant. There were significant differences in volume and volume difference between oleic acid NaHCO_3 group and oleic acid NaHCO_3 group (volume: P = 0.631, volume difference: 0.212). The results of HE staining showed that oleic acid group and oleic acid NaHCO_3 group obviously had calcium deposition. See obvious calcium deposit. Conclusion Local hyperoleic acid can promote tumor calcium deposition, oleic acid can inhibit tumor growth, local high NaHCO_3 can inhibit tumor growth, but tumor volume will continue to increase after discontinuation of NaHCO_3, which may be related to NaHCO_3 changing the acidic environment of tumor. Oleic acid and NaHCO_3 have the tendency of synergistic inhibition of tumor growth.
【作者單位】： 山西醫(yī)科大學第二醫(yī)院
【分類號】：R73-3

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