發(fā)布時(shí)間：2018-07-05 00:20

  本文選題：四溴苯三唑 + 宮頸腫瘤HeLa細(xì)胞��； 參考：《吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年03期

【摘要】：目的:探討四溴苯三唑(TBB)對(duì)人宮頸癌HeLa細(xì)胞的促凋亡作用,闡明其對(duì)相關(guān)信號(hào)轉(zhuǎn)導(dǎo)通路的作用機(jī)制。方法:選取處于對(duì)數(shù)生長(zhǎng)期的人宮頸癌HeLa細(xì)胞,將其分為對(duì)照組(未給予TBB)和實(shí)驗(yàn)組(給予TBB)。采用MTT比色法檢測(cè)HeLa細(xì)胞存活率,采用倒置顯微鏡觀察人宮頸癌HeLa細(xì)胞形態(tài)表現(xiàn),采用AnnexinⅤ-FITC/PI雙染法及流式細(xì)胞術(shù)檢測(cè)HeLa細(xì)胞凋亡率,采用Western blotting法檢測(cè)細(xì)胞中凋亡相關(guān)蛋白p-Akt、Akt、Bcl-2、Bax、cleaved-caspase-3和Pro-caspase-3表達(dá)水平。結(jié)果:MTT法檢測(cè),與對(duì)照組比較,實(shí)驗(yàn)組HeLa細(xì)胞存活率明顯降低(P0.01),且呈濃度依賴性;倒置顯微鏡下觀察,實(shí)驗(yàn)組HeLa細(xì)胞出現(xiàn)體積縮小、細(xì)胞核皺縮等細(xì)胞凋亡的形態(tài)變化;AnnexinⅤ-FITC/PI雙染法和流式細(xì)胞術(shù)檢測(cè),與對(duì)照組比較,實(shí)驗(yàn)組各時(shí)間段(3、6、12和24h)細(xì)胞凋亡率明顯升高(P0.05或P0.01);Western blotting檢測(cè),與對(duì)照組比較,實(shí)驗(yàn)組HeLa細(xì)胞中抗凋亡蛋白p-Akt和Bcl-2表達(dá)水平逐漸降低,促凋亡蛋白Bax和cleaved-caspase-3表達(dá)水平逐漸升高,Pro-caspase-3表達(dá)水平逐漸降低(P0.05或P0.01)。結(jié)論:TBB可能通過(guò)抑制Akt信號(hào)通路的活性進(jìn)而有效促進(jìn)HeLa細(xì)胞凋亡。
[Abstract]:Aim: to investigate the apoptotic effect of tetrabromotriazole (TBB) on human cervical cancer HeLa cells and to elucidate the mechanism of TBB on signal transduction pathway. Methods: human cervical cancer HeLa cells in logarithmic phase were divided into control group (without TBB) and experimental group (TBB). The survival rate of HeLa cells was detected by MTT colorimetry, the morphology of human cervical cancer HeLa cells was observed by inverted microscope, the apoptosis rate of HeLa cells was detected by Annexin 鈪，

本文編號(hào)：2098098