本文選題：地西他濱 + A�。� 參考：《中國醫(yī)科大學學報》2017年12期

【摘要】：目的檢測地西他濱對A431細胞生物學功能的影響并初步探討其機制。方法將0、5、10和20μmol/L的地西他濱分別作用于A431細胞,通過MTT法觀察地西他濱對細胞增殖的影響,通過Western blotting和實時PCR檢測地西他濱作用于A431細胞后細胞增殖相關蛋白Cyclin B1、PCNA和CDC2的改變。通過Hoechst 33258染色檢測0、5和10μmol/L地西他濱對A431細胞凋亡狀態(tài)的影響,采用Western blotting和實時PCR檢測地西他濱對A431細胞凋亡相關蛋白Bax和Bcl-2的影響。采用transwell實驗檢測細胞侵襲轉(zhuǎn)移情況,并通過Western blotting和實時PCR檢測0、5和10μmol/L的地西他濱對侵襲轉(zhuǎn)移相關蛋白MMP2的影響。結(jié)果 MTT檢測發(fā)現(xiàn),5、10和20μmol/L的地西他濱作用于A431細胞24 h后對細胞增殖的抑制率分別為(43.81±1.53)%、(48.64±4.65)%和(50.69±4.99)%,隨著藥物的濃度增加,對A431細胞的增殖抑制率顯著增加(P0.05)。Hoechst 33258染色發(fā)現(xiàn),地西他濱可以顯著促進細胞凋亡,transwell實驗也證明地西他濱可以明顯抑制A431細胞的轉(zhuǎn)移能力。Western blotting和實時PCR結(jié)果顯示,地西他濱影響A431細胞中Cyclin B1、CDC2、PCNA、Bax、Bcl-2和MMP2蛋白及m RNA水平,地西他濱處理組與對照組比較,差異均有統(tǒng)計學意義(P0.05)。結(jié)論地西他濱對外陰鱗狀細胞癌的生物學功能有很強的抑制作用,可以作為治療外陰鱗狀細胞癌的化療藥物。
[Abstract]:Objective to investigate the effect of dietabine on biological function of A 431 cells and its mechanism. Methods the cell proliferation of A431 cells was observed by MTT, and the changes of Cyclin B1P and CDC2 were detected by Western blotting and real-time PCR. The apoptosis of A431 cells was detected by Hoechst 33258 staining, and the apoptosis related proteins Bax and Bcl-2 of A431 cells were detected by Western blotting and real-time PCR. Transwell assay was used to detect the invasion and metastasis of the cells, and the effects of 0 渭 mol/L and 10 渭 mol/L on the invasion and metastasis associated protein MMP2 were detected by Western blotting and real-time PCR. Results the inhibitory rates of dietabine on A431 cell proliferation were 43.81 鹵1.53 鹵4.65% and 50.69 鹵4.99%, respectively. With the increase of drug concentration, the inhibition rate of proliferation of A431 cells was significantly increased with the increase of drug concentration. Hoechst 33258 staining showed that the inhibitory rate of dietabine on A431 cell proliferation was significantly higher than that of control group. Dietabine could significantly promote apoptosis. The transwell assay also demonstrated that dietabine could significantly inhibit the metastatic ability of A431 cells. Western blotting and real-time PCR results showed that dietabine affected the expression of Bcl-2, MMP2 and m RNA in A431 cells. Compared with the control group, the difference between the two groups was statistically significant (P 0.05). Conclusion Detabine has a strong inhibitory effect on the biological function of vulvar squamous cell carcinoma and can be used as a chemotherapeutic drug for vulvar squamous cell carcinoma.
【作者單位】： 中國醫(yī)科大學附屬盛京醫(yī)院婦產(chǎn)科;沈陽醫(yī)學院附屬第二醫(yī)院普外科;
【基金】：沈陽市科學技術計劃(F15-199-1-34)
【分類號】：R737.35

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