本文選題：表皮生長因子 + 外泌體��； 參考：《北京協(xié)和醫(yī)學(xué)院》2016年博士論文

【摘要】：背景肺癌是我國乃至全世界發(fā)病率和死亡率最高的腫瘤,其進展快、惡性程度高且極其容易轉(zhuǎn)移。腫瘤產(chǎn)生的外泌體在腫瘤的發(fā)生、侵襲和轉(zhuǎn)移方面都扮演者重要角色。表皮生長因子受體(Epidermal Growth Factor Receptor, EGFR)的過表達與EGFR信號通路的激活與肺腺癌的增殖、侵襲、轉(zhuǎn)移、密切相關(guān)。但關(guān)于EGFR通路激活對肺腺癌產(chǎn)生的外泌體的影響,目前研究尚不夠。目的觀察表皮生長因子(Epidermal Growth Factor, EGF)刺激對肺腺癌細(xì)胞系產(chǎn)生的外泌體形態(tài)、粒徑以及分泌量的影響,并探討EGFR相關(guān)的促腫瘤增殖、轉(zhuǎn)移作用能否能通過外泌體在腫瘤細(xì)胞間傳遞。方法使用EGF刺激人肺腺癌H322細(xì)胞,梯度離心法分離其細(xì)胞上清中外泌體。采用透射電鏡、納米示蹤分析、Western蛋白印記以及外泌體蛋白定量方法鑒定并比較EGF刺激組與對照組所得外泌體在形態(tài)、粒徑以及分泌量方面的區(qū)別。將上述兩組外泌體與肺腺癌細(xì)胞共孵育后,檢測肺腺癌細(xì)胞在增殖和轉(zhuǎn)移方面的變化。結(jié)果梯度離心所得到的顆粒具有外泌體典型杯狀的結(jié)構(gòu),顆粒直徑主要分布在40-150nm之間,表達外泌體標(biāo)志性蛋白CD63和TSG101。EGF處理組與對照組相比,肺腺癌產(chǎn)生的外泌體在形態(tài)、粒徑分布無明顯影響,但分泌量明顯增加。兩組外泌體都可促進肺腺癌細(xì)胞的增殖,但組間無統(tǒng)計學(xué)差異差異。而EGF處理組外泌體較對照組能提高肺腺癌的遷移能力,且具有統(tǒng)計學(xué)意義。結(jié)論EGF刺激對肺腺癌細(xì)胞產(chǎn)生的外泌體的形態(tài)和粒徑影響不明顯,但可促進外泌體的分泌。EGFR相關(guān)促腫瘤轉(zhuǎn)移作用可能通過外泌體在腫瘤細(xì)胞間傳遞。
[Abstract]:Background Lung cancer is a tumor with the highest morbidity and mortality in China and the world. Tumor-producing exocrine plays an important role in tumorigenesis, invasion and metastasis. Overexpression of epidermal Growth Factor Receptor, EGFR) and activation of EGFR signaling pathway are closely related to proliferation, invasion and metastasis of lung adenocarcinoma. However, the effect of EGFR pathway activation on exocrine secretion in lung adenocarcinoma is not well studied. Objective to observe the effects of epidermal Growth Factor, EGF) stimulation on the morphology, size and secretion of exocrine in lung adenocarcinoma cell lines, and to explore the role of EGFR in promoting tumor proliferation and whether metastasis can be transmitted between tumor cells through exocrine. Methods Human lung adenocarcinoma cell line H 322 was stimulated by EGF and its supernatant was isolated by gradient centrifugation. Transmission electron microscopy, nano-tracer analysis and quantitative analysis of Western protein imprinting and exocrine protein were used to identify and compare the morphology, particle size and secretion of exocrine in EGF stimulated group and control group. After the two groups were incubated with lung adenocarcinoma cells, the proliferation and metastasis of lung adenocarcinoma cells were detected. Results the granules obtained by gradient centrifugation had the typical goblet structure of exocrine, and the diameter of the granules mainly distributed between 40-150nm. The exocrine bodies produced by lung adenocarcinoma were in shape in the treatment group of CD63 and TSG101.EGF, which expressed the hallmark protein of exocrine, compared with the control group. The particle size distribution had no obvious effect, but the secretion increased obviously. Both exocrine bodies could promote the proliferation of lung adenocarcinoma cells, but there was no statistical difference between the two groups. The exocrine body in EGF group could improve the migration ability of lung adenocarcinoma compared with the control group, and had statistical significance. Conclusion EGF stimulation has no significant effect on the morphology and particle size of exocrine bodies produced by lung adenocarcinoma cells, but it can promote the secretion of exocrine. EGFR-related tumor metastasis may be transmitted between tumor cells through exosomes.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級別】：博士
【學(xué)位授予年份】：2016
【分類號】：R734.2

【相似文獻】

相關(guān)期刊論文 前10條

1 楊晉平,王澤,張?zhí)K;肺腺癌5-羥色胺受體的免疫組織化學(xué)定位[J];四川解剖學(xué)雜志;2003年02期

2 吳一龍,林嘉穎,楊學(xué)寧,喬貴賓,王坤,陳剛;肺腺癌患者酪氨酸激酶信號傳導(dǎo)通路的異常與臨床預(yù)后[J];中華醫(yī)學(xué)雜志;2004年12期

3 張利群,陳杭薇,王四海,辛慶紅,杜玉國,尤蘭華;人呼吸道合胞病毒轉(zhuǎn)錄調(diào)節(jié)基因轉(zhuǎn)染肺腺癌細(xì)胞系的研究[J];山東醫(yī)藥;2005年12期

4 黎聯(lián);梅同華;周向東;張新高;車德亞;;細(xì)胞外信號調(diào)節(jié)激酶信號通路與基質(zhì)金屬蛋白酶-26在肺腺癌表達中的關(guān)系[J];第三軍醫(yī)大學(xué)學(xué)報;2009年02期

5 王亞南;顧國浩;;生物芯片在肺腺癌研究和診療中的應(yīng)用進展[J];國際檢驗醫(yī)學(xué)雜志;2011年02期

6 ;我國人體肺腺癌細(xì)胞系建立[J];腫瘤;1981年06期

7 季晨陽,張義R，

本文編號：1961604