本文選題：胃腫瘤 + 白介素-17(IL-17)　； 參考：《青島大學(xué)》2017年碩士論文

【摘要】：研究目的研究胃癌發(fā)生的分子遺傳機(jī)制,為胃癌的預(yù)防、胃癌高危人群的篩查、診斷提供依據(jù),也能對(duì)胃癌的治療提供新的思路。探討IL-17基因rs2275913、rs763780、rs3748067位點(diǎn)多態(tài)性與胃癌易感性的相關(guān)性。研究方法采取病例對(duì)照研究,收集2014年12月—2015年12月青島市市立醫(yī)院355例漢族胃癌患者組成胃癌組,選取同期在體檢中心進(jìn)行健康體檢的300名正常者組成對(duì)照組。兩組人群均為漢族人口。采集兩組患者外周血液,參照血液基因組DNA提取試劑盒(天根生化科技有限公司)的說明書提取全血基因組DNA,PCR擴(kuò)增目的基因片段,采用DNA直接測序法檢測IL-17基因rs2275913、rs763780、rs3748067位點(diǎn)基因型,探討其位點(diǎn)多態(tài)性與胃癌發(fā)病風(fēng)險(xiǎn)之間的關(guān)系。結(jié)果胃癌組與對(duì)照組IL-17基因rs2275913位點(diǎn)基因型分布差異有統(tǒng)計(jì)學(xué)意義(cc2=17.192,P=0.000);與GG基因型相比,攜帶AA基因型的個(gè)體胃癌發(fā)病風(fēng)險(xiǎn)增加,差異有統(tǒng)計(jì)學(xué)意義(c2=16.829,P0.05;OR=2.891,95%CI=1.721-4.857);攜帶GA基因型的個(gè)體胃癌發(fā)病風(fēng)險(xiǎn)增加(OR=1.172,95%CI=0.841-1.634),但差異無統(tǒng)計(jì)學(xué)意義(c2=0.878,P0.05)。與攜帶rs763780位點(diǎn)CC基因型相比,攜帶CT基因型的個(gè)體胃癌易感性增高(OR=1.132,95%CI=0.810-1.583),但是差異沒有統(tǒng)計(jì)學(xué)意義(c2=0.526,P0.05),攜帶TT基因型的個(gè)體胃癌易感性增高(OR=1.404,95%CI=0.755-2.609),差異也沒有統(tǒng)計(jì)學(xué)意義(c2=1.156,P0.05)。與攜帶rs3748067位點(diǎn)TT基因型相比,攜帶CT基因型的個(gè)體胃癌易感性增高(OR=1.402,95%CI=0.748-1.451),但是差異沒有統(tǒng)計(jì)學(xué)意義(c2=0.059,P0.05),攜帶CC基因型的個(gè)體胃癌易感性降低(OR=0.661,95%CI=0.408-1.072),差異也沒有統(tǒng)計(jì)學(xué)意義(c2=2.830,P0.05)。對(duì)于rs2275913位點(diǎn),H.Pylori陽性組AA基因型頻率明顯高于H.Pylori陰性組(18.2%比8.5%),差異有統(tǒng)計(jì)學(xué)意義(P0.05);而H.Pylori陽性組GA、GG基因型頻率與H.Pylori陰性組相比,差異無統(tǒng)計(jì)學(xué)意(P0.05)。對(duì)于rs3748067位點(diǎn),H.Pylori陽性組與H.Pylori陰性組基因型分布無明顯差異(P0.05)。結(jié)論IL-17基因rs2275913 A等位基因的攜帶會(huì)增加胃癌發(fā)病風(fēng)險(xiǎn),IL-17基因rs763780、rs3748067位點(diǎn)多態(tài)性與胃癌發(fā)病風(fēng)險(xiǎn)無明顯相關(guān)性。
[Abstract]:Objective to study the molecular genetic mechanism of gastric cancer, to provide evidence for the prevention of gastric cancer, screening and diagnosis of high risk population of gastric cancer, and to provide new ideas for the treatment of gastric cancer. To investigate the association between the polymorphism of rs2275913, rs763780, rs3748067 and the susceptibility of gastric cancer. Methods A case-control study was conducted to collect 355 patients with gastric cancer of Han nationality from December 2014 to December 2015 in Qingdao City Hospital. Both groups were Han population. The peripheral blood fluid of two groups of patients was collected and the target gene fragment was amplified by PCR with reference to the instructions of the blood genomic DNA extraction kit. The genotypes of IL-17 gene rs2275913, rs763780, rs3748067 were detected by DNA direct sequencing. To investigate the relationship between locus polymorphism and the risk of gastric cancer. Results the genotype distribution of rs2275913 locus of IL-17 gene in gastric cancer group was significantly different from that in control group, and the risk of gastric cancer was increased in individuals with AA genotype compared with GG genotype. The difference was statistically significant (P < 0.05). The risk of gastric cancer in individuals with GA genotype increased by 1.172 ~ 95CIN 0.841-1.634, but there was no significant difference between 0.878P0.05and 0.878P0.05.The difference was statistically significant (P < 0.05), but there was no significant difference in the risk of gastric cancer in individuals with GA genotype (P = 0.878P0.05), but there was no significant difference in the risk of gastric cancer in individuals with GA genotype (P < 0.05), but there was no significant difference between the two groups. Compared with CC genotype carrying rs763780 locus, the susceptibility of individuals with CT genotype was higher than that of individuals with CT genotype. The susceptibility of individuals with CT genotype was 0.810-1.583, but the difference was not statistically significant, but there was no significant difference in the susceptibility of individuals with TT genotype. The susceptibility of individuals with TT genotype was 0.755-2.609, and the difference was not statistically significant. Compared with TT genotype carrying rs3748067 locus, the susceptibility of individuals carrying CT genotype to gastric cancer was increased. The susceptibility of individuals with CT genotype was 0.748-1.451C, but the difference was not statistically significant. The susceptibility of individuals with CC genotype was lower than that of individuals with rs3748067 locus TT genotype (CI 0.408-1.072), and there was no significant difference between them. The frequency of AA genotype in rs2275913 positive group was significantly higher than that in H.Pylori negative group (18.2% vs 8.5%), the difference was statistically significant (P 0.05), but there was no significant difference between H.Pylori positive group and H.Pylori negative group. There was no significant difference in genotype distribution between H.Pylori positive group and H.Pylori negative group for rs3748067 locus. Conclusion carrying the rs2275913 A allele of IL-17 gene may increase the risk of gastric cancer. The polymorphism of rs763780 rs3748067 in IL-17 gene has no significant correlation with the risk of gastric cancer.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 聶剛;;成纖維細(xì)胞生長因子2基因多態(tài)性與胃癌的關(guān)聯(lián)性研究[J];現(xiàn)代醫(yī)藥衛(wèi)生;2017年03期

2 蔡慧;丁一波;馬立業(yè);杜琰;曹廣文;;炎癥信號(hào)網(wǎng)絡(luò)及其關(guān)鍵分子事件在胃癌進(jìn)化發(fā)育中的作用[J];東南國防醫(yī)藥;2016年03期

3 陳俊利;溫瑩;常虹;李思齊;趙蕓;薛清萍;王英;李琛;趙志梅;潘雄飛;楊春霞;;IL-1B和IL-1RN基因多態(tài)性與非賁門胃癌發(fā)病風(fēng)險(xiǎn)的研究[J];現(xiàn)代預(yù)防醫(yī)學(xué);2016年02期

4 王勝;張蕊;王俊鳳;趙輝;;Th17細(xì)胞及IL-17在非小細(xì)胞肺癌患者外周血的表達(dá)及臨床意義[J];實(shí)用臨床醫(yī)藥雜志;2015年23期

5 李巧霞;李清靖;劉艷軍;陳錦霞;李宏;蘇景偉;張超;趙雪峰;單保恩;;Th17細(xì)胞及其相關(guān)細(xì)胞因子在胃癌中的表達(dá)及臨床意義[J];腫瘤學(xué)雜志;2015年09期

6 Zi-Wen Long;Hong-Mei Yu;Ya-Nong Wang;Dan Liu;Yan-Zhi Chen;Yu-Xia Zhao;Lu Bai;;Association of IL-17 polymorphisms with gastric cancer risk in Asian populations[J];World Journal of Gastroenterology;2015年18期

7 陸興熱;陳鳳羽;楊德興;劉孝文;袁雕;資云菊;何祥;和潤濘;;IL-1β基因多態(tài)性及幽門螺桿菌感染交互作用與胃癌發(fā)生的相關(guān)性研究[J];中華醫(yī)院感染學(xué)雜志;2015年10期

8 李云婷;郭欣;李彥會(huì);汪冉;;白細(xì)胞介素4和白細(xì)胞介素10基因多態(tài)性與胃癌易感性的關(guān)系[J];臨床薈萃;2015年05期

9 張健;魏威;應(yīng)榮超;項(xiàng)晶晶;金慧成;馬勝林;;幽門螺桿菌感染對(duì)胃癌患者血清、胃液及組織IL-6、IL-8水平影響的臨床研究[J];中華醫(yī)院感染學(xué)雜志;2015年07期

10 陶梅;張玲霞;宋瑛;莊坤;張寧霞;張瀝;;TNF-β+252基因多態(tài)性及幽門螺桿菌感染與胃癌易感性的關(guān)系[J];山西醫(yī)科大學(xué)學(xué)報(bào);2014年12期

，

本文編號(hào)：1958985