本文選題：結(jié)腸癌 + SIRT1　； 參考：《延邊大學(xué)》2017年碩士論文

【摘要】：目的:探討SIRT1(silent information regulator 1,沉默信息調(diào)節(jié)因子1)和突變型p53在結(jié)腸癌組織及癌旁組織中的表達(dá)意義,分析兩種蛋白的表達(dá)與結(jié)腸癌患者臨床病理特征的關(guān)系,并對(duì)SIRT1、突變型p53在結(jié)腸癌組織中的表達(dá)進(jìn)行相關(guān)性分析。方法:1.收集病理診斷為結(jié)腸癌的癌組織和癌旁組織石蠟包埋組織標(biāo)本各58例,常規(guī)進(jìn)行HE染色,采用免疫組織化學(xué)方法檢測(cè)SIRT1、突變型p53在結(jié)腸癌組織及癌旁組織中的表達(dá)。2.分析SIRT1和突變型p53在結(jié)腸癌組織中的表達(dá)與臨床病理特征的關(guān)系。3.分析SIRT1與突變型p53在結(jié)腸癌組織表達(dá)的相關(guān)性。結(jié)果:1.SIRT1在結(jié)腸癌組織中主要定位于細(xì)胞核,少量定位于胞漿,在癌組織中的陽性表達(dá)率為43.1%(25/58),在癌旁組織中的陽性表達(dá)率為7%(4/58),兩者之間差異有統(tǒng)計(jì)學(xué)意義(P0.05)。2.突變型p53在結(jié)腸癌組織中全部定位于細(xì)胞核,在癌組織中的陽性表達(dá)率為51.7%(30/58),在癌旁組織中陽性表達(dá)率為0%(0/58),兩者之間差異有統(tǒng)計(jì)學(xué)意義(P0.05)。3.SIRT1在結(jié)腸癌中的表達(dá)與年齡、分化程度有關(guān)(P0.05),與性別、民族、腫瘤大小、浸潤深度、淋巴結(jié)轉(zhuǎn)移、遠(yuǎn)處轉(zhuǎn)移、TNM分期均無關(guān)(P0.05)。4.突變型p53在結(jié)腸癌中的表達(dá)與年齡有關(guān)(P0.05),與性別、民族、腫瘤大小、分化程度、浸潤深度、遠(yuǎn)處轉(zhuǎn)移、TNM分期均無關(guān)(P0.05)。5.SIRT1在結(jié)腸癌組織中的表達(dá)與突變型p53在結(jié)腸癌組織中的表達(dá)呈正相關(guān),相關(guān)系數(shù)為0.417,P=0.0010.05。結(jié)論:1.SIRT1在結(jié)腸癌組織中高表達(dá),與患者年齡、分化程度有關(guān)。2.p53在結(jié)腸癌組織中高表達(dá),與年齡有關(guān)。3.結(jié)腸癌中SIRT1與突變型p53呈正相關(guān),SIRT1與p53可能起協(xié)同作用促進(jìn)結(jié)腸癌的發(fā)生。
[Abstract]:Objective: to investigate the expression significance of SIRT1(silent information regulator 1 (silencing signal regulator 1) and mutant p53 in colon cancer tissues and adjacent tissues, and to analyze the relationship between the expression of these two proteins and the clinicopathological features of colon cancer patients. The expression of SIRT1 and mutant p53 in colon cancer tissues was analyzed. Method 1: 1. The paraffin embedded tissues of 58 cases of carcinoma and 58 cases of paracancerous tissues were collected for HE staining. The expression of SIRT1 and mutant p53 in colon cancer and adjacent tissues were detected by immunohistochemical method. To analyze the relationship between the expression of SIRT1 and mutant p53 and the clinicopathological features of colon cancer. To analyze the correlation between SIRT1 and mutant p53 expression in colon cancer. Results: 1. SIRT1 was mainly located in the nucleus of colon cancer tissue, and a little in the cytoplasm. The positive expression rate of SIRT1 in cancer tissue was 43.1 / 58 and that in paracancerous tissue was 74.58%. The difference between the two groups was statistically significant (P 0.05 / 58). The positive expression rate of mutant p53 in colon cancer tissues was 51.7% and 30 / 58% respectively, and the positive expression rate in adjacent tissues was 0% / 58%. There was a significant difference between the two groups in the expression and age of SIRT1 in colon cancer. The degree of differentiation was related to P0.05, but not to sex, nationality, tumor size, depth of invasion, lymph node metastasis, TNM stage of distant metastasis. The expression of mutant p53 in colon cancer is related to age, sex, nationality, tumor size, differentiation, depth of invasion. There was no significant correlation between TNM stage of distant metastasis and mutant p53 expression in colon cancer tissues, and the correlation coefficient was 0.417% P ~ (0.05) P ~ (0.0010.05). 5. The expression of SIRT1 was positively correlated with the expression of mutant p53 in colon cancer tissues. Conclusion: 1. The overexpression of SIRT1 in colon cancer tissues is related to the age and differentiation degree of patients. 2. P53 expression in colon cancer tissue is higher than that in cancer tissue, and it is related to age. There is a positive correlation between SIRT1 and mutant p53 in colon cancer. SIRT1 and p53 may play a synergistic role in promoting the occurrence of colon cancer.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.35

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