本文選題：乳腺癌 + 危險因素�。� 參考：《寧夏醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的:調(diào)查分析乳腺癌的環(huán)境危險因素,研究rs6788895(SIAH2基因)、rs10941679(5p12)、rs889312(MAP3K1)、rs13387042(2q35)、rs6504950(17q23)、rs17530068(6q14)和rs2284378(20q11)7個位點的單核苷酸多態(tài)性與寧夏女性乳腺癌的關(guān)系,對環(huán)境因素和以上7個位點進(jìn)行交互作用分析,探討基因-環(huán)境交互作用對乳腺癌的影響。方法:采用以醫(yī)院為基礎(chǔ)且以年齡為頻數(shù)匹配條件的病例-對照研究方法,共收集病例組與對照組研究對象各225人,其中病例組來源于2013年5月至2014年7月于寧夏醫(yī)科大學(xué)附屬總醫(yī)院就診入院的新發(fā)寧夏女性乳腺癌患者;對照組來源于同一醫(yī)院同期入院的無乳腺癌的女性患者。研究對象使用統(tǒng)一的調(diào)查表進(jìn)行流行病學(xué)調(diào)查,調(diào)查內(nèi)容主要包括一般人口學(xué)資料、月經(jīng)史、生育史、生活習(xí)慣和腫瘤家族史等其他乳腺癌易感環(huán)境因素。同時采集研究對象的靜脈血2ml,采用Sequenom Mass Array系統(tǒng)進(jìn)行基因型檢測,所有統(tǒng)計計算均由SPSS17.0統(tǒng)計軟件完成。結(jié)果:1.婚姻狀況、文化程度、年齡、民族、職業(yè)及熬夜情況在病例組與對照組之間的分布差異均無統(tǒng)計學(xué)意義(P0.05)。2.病例組與對照組研究對象在初婚年齡、月經(jīng)持續(xù)天數(shù)、首次生育年齡、生育次數(shù)、流產(chǎn)次數(shù)、是否絕經(jīng)、月經(jīng)規(guī)律性以及乳腺自檢方面的分布差異具有統(tǒng)計學(xué)意義(P0.05);兩組研究對象在初潮年齡、月經(jīng)周期、末次生育年齡、絕經(jīng)年齡、哺乳年限、行經(jīng)年限、是否避孕、醫(yī)院乳腺檢查、是否哺乳、及BMI方面的分布差異均無統(tǒng)計學(xué)意義(P0.05)。3.體育鍛煉及晚上佩戴文胸情況在病例組與對照組之間的分布差異有統(tǒng)計學(xué)意義(P0.05);而吸煙、被動吸煙、飲酒、失眠情況、午睡情況和飲食習(xí)慣方面在兩組中分布差異均無統(tǒng)計學(xué)意義(P0.05)。4.癌癥家族史在病例組與對照組間的分布差異具有統(tǒng)計學(xué)意義(P0.05),且病例組中有癌癥家族史人群所占比例(9.8%)顯著高于對照組(1.8%)。5.經(jīng)logistic回歸分析發(fā)現(xiàn)流產(chǎn)次數(shù)(OR=1.565)和癌癥家族史(OR=5.030)為乳腺癌的危險性因素,乳腺自檢(OR=0.418)、體育鍛煉(OR=0.444)和晚上不佩戴文胸(OR=0.562)為可能會降低患乳腺癌的保護(hù)性因素。6.rs6788895、rs10941679、rs889312、rs13387042、rs6504950、rs17530068和rs2284378位點的基因型頻率及等位基因頻率在病例組與對照組間的分布差異均無統(tǒng)計學(xué)意義。7.rs6788895、rs889312、rs13387042、rs6504950、rs17530068、rs10941679和rs2284378位點多態(tài)性與環(huán)境因素(流產(chǎn)次數(shù)、癌癥家族史、乳腺自檢、體育鍛煉、晚上是否佩戴文胸)之間不存在交互效應(yīng)(P0.05)。結(jié)論:1.流產(chǎn)次數(shù)、乳腺自檢、體育鍛煉、晚上佩戴文胸和癌癥家族史可能是女性乳腺癌的環(huán)境影響因素。2.rs6788895、rs10941679、rs889312、rs13387042、rs6504950、rs17530068和rs2284378位點的多態(tài)性與乳腺癌的發(fā)生可能不相關(guān)。3.s6788895、rs10941679、rs889312、rs13387042、rs6504950、rs17530068和rs2284378位點和環(huán)境因素可能不存在交互效應(yīng)。
[Abstract]:Objective: To investigate and analyze the environmental risk factors of breast cancer, and to study the relationship between single nucleotide polymorphism of rs6788895 (SIAH2 gene), rs10941679 (5p12), rs889312 (MAP3K1), rs6504950 (2q35), rs6504950 (17q23), rs17530068 (6q14) and 7 loci with female breast cancer in Ningxia, and the interaction between the environmental factors and the above 7 loci. The effect of gene environmental interaction on breast cancer was analyzed. Methods: a case-control study based on the hospital based and matched condition of age was used to collect 225 people from the case group and the control group. The case group came from May 2013 to July 2014 at the General Hospital of Ningxia Medical University. A new Ningxia female breast cancer patient was admitted to the hospital. The control group was derived from women who had no breast cancer in the same hospital during the same period. The subjects used a unified questionnaire for epidemiological investigation, including general demographic data, menstrual history, birth history, life habits and family history of cancer. At the same time, the venous blood 2ml of the subjects was collected and the Sequenom Mass Array system was used for genotyping. All the statistical calculations were completed by the SPSS17.0 software. Results: there was no significant difference in the distribution difference between the case group and the control group in 1. marital status, cultural degree, age, nationality, occupation and staying up. (P0.05 The.2. case group and the control group were studied at the age of first marriage, the duration of menstruation, the first birth age, the number of birth, the number of abortion, the menopause, the regularity of menstruation and the difference in the distribution of breast self examination (P0.05); the two groups of subjects were at the age of menarche, the menstrual cycle, the last birth age, the menopause age, lactation. Years, menstruation, contraception, hospital breast examination, breast feeding, and BMI distribution were not statistically significant (P0.05).3. physical exercise and wearing a bra at night between the case group and the control group had a statistically significant difference (P0.05), while smoking, passive smoking, drinking, insomnia, nap and diet The distribution difference between the two groups was not statistically significant (P0.05).4. cancer family history between the case group and the control group had statistical significance (P0.05), and the proportion of the family history of cancer (9.8%) in the case group was significantly higher than the control group (1.8%).5. by logistic regression analysis to find the frequency of abortion (OR=1.565) and cancer. Family history of the disease (OR=5.030) is a risk factor for breast cancer, breast self examination (OR=0.418), physical exercise (OR=0.444) and not wearing a bra at night (OR=0.562) may reduce the protective factors of breast cancer, the genotype frequency and allele frequency of.6.rs6788895, rs10941679, rs889312, rs13387042, rs6504950, rs17530068, and rs2284378 loci. There was no significant difference in the distribution of.7.rs6788895, rs889312, rs13387042, rs6504950, rs17530068, rs10941679, and rs2284378 locus polymorphism and environmental factors (abortion times, cancer family history, breast self examination, physical exercise, and whether to wear a bra at night). There was no interaction effect (P0.05). Conclusion: 1. The number of miscarriages, self examination of breast, physical exercise, the history of wearing a bra and a family history of cancer at night may be the environmental factors of female breast cancer.2.rs6788895, rs10941679, rs889312, rs13387042, rs6504950, rs17530068 and rs2284378 polymorphism and breast cancer can not be associated with.3.s6788895, rs10941679, rs889312, rs13387042, rs6504 950, there may be no interaction effect between rs17530068 and rs2284378 loci and environmental factors.

【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R737.9

【引證文獻(xiàn)】

相關(guān)會議論文 前1條

1 李海欣;陳可欣;溫萬青;鄭葦;;乳腺癌遺傳易感位點的基因環(huán)境交互作用的研究[A];全國腫瘤流行病學(xué)和腫瘤病因?qū)W學(xué)術(shù)會議論文集[C];2011年

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本文編號：1870714