本文選題：胃癌 + 即刻早期反應(yīng)X-��； 參考：《中國現(xiàn)代醫(yī)學(xué)雜志》2017年20期

【摘要】：目的揭示即刻早期反應(yīng)X-1(IEX-1)蛋白在胃癌組織及細胞系中的表達,探討其對人胃癌細胞凋亡和增殖的影響。方法采用免疫組織化學(xué)法檢測100例胃鏡活檢標(biāo)本中IEX-1蛋白的表達,用實時熒光定量聚合酶鏈反應(yīng)和Western blot檢測人正常胃黏膜細胞GES-1和胃癌細胞MKN28中IEX-1基因和蛋白的表達,同時轉(zhuǎn)染IEX-1的過表達質(zhì)粒pc DNA3-FLAG-IEX-1和干擾質(zhì)粒p LL3.7-si-IEX-1,分別采用流式細胞術(shù)及四甲基偶氮唑鹽比色法檢測IEX-1對細胞周期、凋亡及增殖的影響。結(jié)果胃癌組織中IEX-1蛋白表達水平高于正常胃組織和慢性胃炎組織(P0.05),且隨著病情進展,IEX-1表達水平有逐漸升高的趨勢。相對于人正常胃黏膜細胞GES-1,胃癌細胞MKN28中IEX-1基因和蛋白表達更高(P0.05)。與對照組相比,IEX-1蛋白表達增高后S期細胞比例升高,G2期細胞比例降低,細胞增殖率升高,凋亡減少(P0.05),而干擾IEX-1蛋白表達后,G1期細胞升高,G2期細胞比例下降,細胞增殖率下降,細胞凋亡增加(P0.05)。結(jié)論 IEX-1蛋白在胃癌組織中呈高表達,在人胃癌細胞中上調(diào)IEX-1蛋白能促進細胞增殖,發(fā)揮抗凋亡能力,干擾IEX-1蛋白表達能阻滯細胞周期進程,并誘導(dǎo)凋亡。
[Abstract]:Objective to investigate the expression of immediate early reaction X-1nIEX-1 protein in gastric carcinoma tissues and cell lines, and to explore its effect on apoptosis and proliferation of human gastric cancer cells. Methods Immunohistochemical method was used to detect the expression of IEX-1 protein in 100 specimens of gastroscope biopsy, and the expression of IEX-1 gene and protein in human normal gastric mucosal cells (GES-1) and gastric cancer cells (MKN28) were detected by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) and Western blot. The overexpression plasmid PC DNA3-FLAG-IEX-1 and the interference plasmid pLL3.7-si-IEX-1 were transfected into IEX-1 at the same time. The effects of IEX-1 on cell cycle, apoptosis and proliferation were detected by flow cytometry and tetramethylazolium colorimetry, respectively. Results the expression level of IEX-1 protein in gastric cancer was higher than that in normal gastric tissue and chronic gastritis tissue, and the expression level of IEX-1 was gradually increasing with the progression of the disease. Compared with the normal gastric mucosal cells GES-1, the expression of IEX-1 gene and protein in MKN28 of gastric cancer cells was higher than that of normal gastric mucosal cells (GES-1). Compared with the control group, the proportion of S phase cells decreased, cell proliferation rate increased and apoptosis decreased after increasing the expression of IEX-1 protein. However, after interfering with the expression of IEX-1 protein, the proportion of G 2 phase cells decreased and the cell proliferation rate decreased after interfering with the expression of IEX-1 protein. Apoptosis increased P 0.05. Conclusion IEX-1 protein is highly expressed in gastric cancer tissues. Upregulation of IEX-1 protein in human gastric cancer cells can promote cell proliferation, exert anti-apoptosis ability, interfere with the expression of IEX-1 protein can block cell cycle progression and induce apoptosis.
【作者單位】： 中南大學(xué)湘雅醫(yī)學(xué)院附屬�？卺t(yī)院腫瘤化療科;
【基金】：2013年海南省�？谑兄攸c科技計劃項目(No:2013-70)
【分類號】：R735.2

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