本文選題：p-21活化激酶 + 結(jié)直腸腫瘤�。� 參考：《濟(jì)南大學(xué)》2015年碩士論文

【摘要】：背景p-21活化激酶(PAK)是小鳥(niǎo)苷三磷酸酶的下游靶蛋白,在許多基本的細(xì)胞功能中發(fā)揮著重要的作用,包括細(xì)胞骨架重組、細(xì)胞運(yùn)動(dòng)。PAK在多種組織中廣泛表達(dá),在多種類(lèi)型的癌癥中過(guò)度表達(dá)。本研究的目的是探討PAK1與PAK4與結(jié)直腸癌臨床病理特征的關(guān)系。方法 PAK1和PAK4在結(jié)直腸腫瘤患者的高度表達(dá)是通過(guò)RT-PCR、免疫組化進(jìn)行臨床分析。結(jié)果PAK1與PAK4基因在結(jié)直腸癌組織中的相對(duì)表達(dá)水平明顯高于正常組織(P0.01)。 PAK4表達(dá)明顯高于在同一癌組織中的PAK1。PAK1和PAK4表達(dá)隨癌組織的臨床分期增加而逐漸升高(P0.01)。PAK1在淋巴結(jié)陽(yáng)性的患者中高表達(dá),PAK4在浸潤(rùn)漿膜層患者中高表達(dá)(P0.05)。PAK1高表達(dá)組與PAK1低表達(dá)組相比有較高的復(fù)發(fā)轉(zhuǎn)移率。后續(xù)分析顯示,中位無(wú)進(jìn)展生存時(shí)間的PAK1高表達(dá)組較Pakl的低表達(dá)組明顯縮短。結(jié)論P(yáng)AK1與PAK4的表達(dá)與結(jié)直腸癌的浸潤(rùn)和轉(zhuǎn)移相關(guān),PAK1的高表達(dá)可能表明大腸癌預(yù)后不良。
[Abstract]:Background p-21 activated kinase (pak) is a downstream target protein of guanosine triphosphatase, which plays an important role in many basic cellular functions, including cytoskeleton recombination, cell motility, PAK expression in a variety of tissues. Overexpression in many types of cancer. The purpose of this study was to investigate the relationship between PAK1 and PAK4 and clinicopathological features of colorectal cancer. Methods the high expression of PAK1 and PAK4 in colorectal cancer patients was analyzed by RT-PCR and immunohistochemistry. Results the relative expression levels of PAK1 and PAK4 genes in colorectal carcinoma were significantly higher than those in normal tissues (P 0.01). The expression of PAK4 was significantly higher than that of PAK1.PAK1 and PAK4 in the same cancer tissue. With the increase of clinical stage of cancer tissue, the expression of p0. 01 and PAK1 in lymph node positive patients was significantly higher. The expression of p0. 05 and PAK1 in infiltrating serous layer patients was higher than that in the high expression group of P0. 05 and PAK1. The recurrence and metastasis rate of low expression group was higher than that of low expression group. The follow-up analysis showed that the high expression group of PAK1 with median survival time without progression was significantly shorter than the group with low expression of Pakl. Conclusion the expression of PAK1 and PAK4 may be associated with the invasion and metastasis of colorectal cancer and the high expression of PAK1 may indicate that the prognosis of colorectal cancer is poor.
【學(xué)位授予單位】：濟(jì)南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R735.34

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