本文選題：電離輻射 + 肺癌　； 參考：《中國(guó)病理生理雜志》2017年05期

【摘要】：目的:探討電離輻射對(duì)肺癌A549細(xì)胞上皮間質(zhì)轉(zhuǎn)化(EMT)的影響及其可能機(jī)制。方法:采用不同劑量(0 Gy、1 Gy、2 Gy、4 Gy和8 Gy)的X射線分別照射肺癌A549細(xì)胞不同時(shí)間,通過(guò)倒置顯微鏡觀察X射線照射12 h、24 h和48 h時(shí)肺癌A549細(xì)胞形態(tài)的改變;Western blot檢測(cè)X射線照射12 h與24 h時(shí)EMT相關(guān)蛋白vimentin、N-cadherin及E-cadherin和轉(zhuǎn)錄因子c-Myc的表達(dá)。結(jié)果:照射后肺癌A549細(xì)胞輪廓不清,突起增多,邊緣不規(guī)則且呈煎蛋樣塌陷,以8 Gy X射線照射48 h時(shí)肺癌A549細(xì)胞上皮間質(zhì)化形態(tài)最明顯。與0 Gy照射劑量對(duì)照組相比,vimentin雖然于4 Gy劑量照射12 h時(shí)下調(diào),但是處理24 h時(shí)各劑量照射組vimentin均表現(xiàn)為上調(diào),其中2 Gy劑量照射組其上調(diào)最明顯(P0.01);與0 Gy照射劑量對(duì)照組相比,1 Gy、2 Gy及4 Gy照射組照射肺癌A549細(xì)胞24 h后其N(xiāo)-cadherin表達(dá)上調(diào)(P0.05);各照射劑量對(duì)肺癌A549細(xì)胞E-cadherin表達(dá)沒(méi)有顯著影響。照射24 h后肺癌A549細(xì)胞c-Myc表達(dá)上調(diào),以4 Gy劑量照射組表達(dá)差異最明顯(P0.01)。結(jié)論:X線電離輻射可能通過(guò)上調(diào)c-Myc表達(dá)促進(jìn)肺癌A549細(xì)胞發(fā)生上皮間質(zhì)轉(zhuǎn)化。
[Abstract]:Aim: to investigate the effect of ionizing radiation on epithelial mesenchymal transformation (EMTT) of lung cancer cell line A549 and its possible mechanism. Methods: lung cancer A549 cells were irradiated with different doses of X ray (1 Gy, 2 Gy, 4 Gy and 8 Gy) for different time, respectively. The morphologic changes of lung cancer A549 cells at 12h and 48h after irradiation were observed by inverted microscope. The expression of EMT related protein vimentininN-cadherin, E-cadherin and transcription factor c-Myc were detected by Western blot at 12h and 24h after X-ray irradiation. Results: the outline of lung cancer A549 cells was not clear, the processes increased, the edges were irregular and collapsed like eggs. The epithelial interstitial morphology of lung cancer A549 cells was the most obvious after 48 h irradiation with 8 Gy X-ray. Compared with the 0 Gy radiation dose control group, vimentin was down-regulated at the dose of 4 Gy for 12 h, but the vimentin was up-regulated in each dose group at 24 h. The expression of N-cadherin was up-regulated in A549 cells 24 h after irradiation with 2 Gy dose of 2 Gy and 4 Gy respectively, and the expression of E-cadherin in A549 cells of lung cancer was not significantly affected by different doses of irradiation. The expression of c-Myc in lung cancer A549 cells was up-regulated 24 hours after irradiation, and the difference was most obvious in the 4 Gy irradiation group. ConclusionIonizing radiation may promote epithelial mesenchymal transformation of lung cancer A549 cells by upregulating the expression of c-Myc.
【作者單位】： 中山大學(xué)附屬第一醫(yī)院呼吸內(nèi)科;中山大學(xué)中山醫(yī)學(xué)院病理生理教研室;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(No.81502492) 廣東省自然科學(xué)基金資助項(xiàng)目(No.2016A030313839)
【分類(lèi)號(hào)】：R363.1+2;;R730.23

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