發(fā)布時(shí)間：2018-04-18 09:00

  本文選題：miR-21 + miR-375　； 參考：《新疆醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:探討微小RNA-21、微小RNA-375在維吾爾族、哈薩克族及漢族食管鱗狀細(xì)胞癌患者組織及血漿中的表達(dá)情況及發(fā)病風(fēng)險(xiǎn)。方法:選取180例食管鱗癌患者,并經(jīng)術(shù)后病理確診,術(shù)前采集其血液標(biāo)本,術(shù)后分別采集食管癌及癌旁組織,實(shí)驗(yàn)方法采用實(shí)時(shí)熒光定量PCR法檢測hsa-miR-21-5p、hsa-miR-375在組織及血液標(biāo)本中的表達(dá)情況;應(yīng)用配對樣本的卡方檢驗(yàn)分析癌和癌旁組織中miR-21和miR-375的表達(dá)差異;應(yīng)用單因素方差分析血漿中miR-21和miR-375在不同民族間的表達(dá)水平。結(jié)果:miR-21在食管組織中表達(dá)水平較癌旁組織高(P0.05),食管鱗癌組織中miR-375表達(dá)水平較癌旁組織低(P0.05),但3個(gè)民族間無明顯差異;哈薩克族和維吾爾族食管鱗癌患者血漿中miR-21表達(dá)水平較漢族高(P0.05);miR-21高表達(dá)及miR-375的低表達(dá)增加了食管癌的發(fā)病風(fēng)險(xiǎn),可能是影響食管癌的危險(xiǎn)因素。結(jié)論:miR-21、miR-375參與食管鱗狀細(xì)胞癌的發(fā)生、發(fā)展,miR-21、miR-375或可成為食管癌早期診斷的潛在腫瘤標(biāo)志物。
[Abstract]:Objective: to investigate the expression and risk of microRNA-21 and RNA-375 in tissues and plasma of Uygur, Kazak and Han patients with esophageal squamous cell carcinoma.Methods: 180 cases of esophageal squamous cell carcinoma were selected and confirmed by pathology. Blood samples were collected before operation, and esophageal carcinoma and adjacent tissues were collected respectively after operation.Methods the expression of hsa-miR-21-5phsa-miR-375 in tissues and blood samples was detected by real-time fluorescence quantitative PCR, and the difference between miR-21 and miR-375 expression in cancer and adjacent tissues was analyzed by chi-square test.Univariate ANOVA was used to analyze the expression of miR-21 and miR-375 in different ethnic groups.Results the expression of miR-375 in esophageal squamous cell carcinoma was lower than that in paracancerous tissue (P 0.05), but there was no significant difference among the three ethnic groups.The higher expression of miR-21 and the lower expression of miR-375 in the plasma of Kazak and Uygur patients with esophageal squamous cell carcinoma increased the risk of esophageal cancer, which may be the risk factors of esophageal cancer.Conclusion miR-21 miR-375 may be involved in the development of esophageal squamous cell carcinoma and may be a potential tumor marker for early diagnosis of esophageal carcinoma.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.1

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