本文選題：PES1 + EGFR��； 參考：《青島大學(xué)》2017年碩士論文

【摘要】：目的檢測(cè)PES1及EGFR在胃癌組織中的表達(dá),探討PES1和EFGR與胃癌的各種臨床病理特征的關(guān)系及兩者在胃癌組織中表達(dá)的相關(guān)性。方法收集60例胃癌患者的外科手術(shù)切除標(biāo)本(60例患者均無(wú)腫瘤家族史,無(wú)遺傳病史,術(shù)前均未曾接受過(guò)化療及放療等抗癌治療措施),取材、包埋、切片后,進(jìn)行常規(guī)HE染色,整理患者的年齡、性別、腫瘤分化程度、腫瘤浸潤(rùn)深度、臨床病理分期、淋巴結(jié)轉(zhuǎn)移情況等臨床病理資料。根據(jù)HE染色切片的診斷結(jié)果,選定典型的蠟塊,采用手工S-P兩步法進(jìn)行免疫組織化學(xué)染色,檢測(cè)PES1和EGFR在胃癌及癌旁組織中的表達(dá)情況。將表達(dá)結(jié)果按照規(guī)定的標(biāo)準(zhǔn)進(jìn)行判讀,整理數(shù)據(jù),將數(shù)據(jù)輸入SPSS17.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)學(xué)的分析處理。結(jié)果①PES1在60例胃癌組織中陽(yáng)性表達(dá)率(31.67%)明顯高于癌旁組織(3.33%),差異具有統(tǒng)計(jì)學(xué)意義(x~2=16.681,P0.05);EGFR在胃癌組織中陽(yáng)性表達(dá)率(75%)也明顯高于癌旁組織(10%),差異具有統(tǒng)計(jì)學(xué)意義(x~2=51.867,P0.05)。②PES1和EGFR在胃癌組織中表達(dá)無(wú)相關(guān)性(r=0.062,P=0.873)。③PES1在胃癌組織中的高表達(dá)與腫瘤的浸潤(rùn)深度(x~2=7.288,P0.05)和臨床病理分期(x~2=8.182,P0.05)密切相關(guān),在侵達(dá)漿膜層者陽(yáng)性率(41.86%)明顯高于未侵達(dá)漿膜層者的陽(yáng)性率(5.88%),在胃癌臨床病理分期為Ⅲ-Ⅳ期患者的陽(yáng)性率(44.74%)明顯高于Ⅰ-Ⅱ期患者的陽(yáng)性率(9.09%);而與患者的年齡(x~2=0.184)、性別(x~2=0.026)、腫瘤分化程度(x~2=0.255)和淋巴結(jié)轉(zhuǎn)移情況(x~2=0.490)無(wú)相關(guān)性(P0.05)。(4)EGFR在胃癌組織中的高表達(dá)與腫瘤的浸潤(rùn)深度(x~2=17.100,P0.05)、臨床病理分期(x~2=27.656,P0.05)和淋巴結(jié)轉(zhuǎn)移(x~2=31.801,P0.05)密切相關(guān),在侵達(dá)漿膜層患者陽(yáng)性率(90.70%)明顯高于未侵達(dá)漿膜層者的陽(yáng)性率(35.29%),在臨床病理分期Ⅲ-Ⅳ期的胃癌患者中的陽(yáng)性率(97.37%)明顯高于Ⅰ-Ⅱ期患者的陽(yáng)性率(36.36%),在有淋巴結(jié)轉(zhuǎn)移者的陽(yáng)性表達(dá)率(93.48%)高于無(wú)淋巴結(jié)轉(zhuǎn)移者的陽(yáng)性率(14.29%);而與患者的性別(x~2=0.030)、年齡(x~2=2.262)、腫瘤分化程度(x~2=0.044)無(wú)相關(guān)性(P0.05)。結(jié)論P(yáng)ES1與EGFR在60例胃癌組織中的陽(yáng)性表達(dá)率均明顯高于癌旁組織;兩者在胃癌組織中的表達(dá)無(wú)相關(guān)性;PES1在胃癌組織中的高表達(dá)與腫瘤浸潤(rùn)深度、臨床病理分期密切相關(guān),而與患者的年齡、性別、腫瘤分化程度、有無(wú)淋巴結(jié)轉(zhuǎn)移等情況無(wú)關(guān);EGFR在胃癌組織中的高表達(dá)與腫瘤的浸潤(rùn)深度、臨床病理分期、淋巴結(jié)轉(zhuǎn)移情況有關(guān),與患者的年齡、性別、腫瘤分化程度等臨床病理參數(shù)無(wú)關(guān)。綜上所述,PES1和EGFR在胃癌的發(fā)生發(fā)展中起重要作用,是胃癌治療的重要靶點(diǎn),同時(shí)也是胃癌預(yù)后評(píng)估的重要參考因素。
[Abstract]:Objective to detect the expression of PES1 and EGFR in gastric cancer and to explore the relationship between PES1 and EFGR and the clinicopathological features of gastric cancer.Methods A total of 60 patients with gastric cancer underwent surgical excision without a family history of cancer, no history of hereditary diseases, no chemotherapy and radiotherapy before operation, and no samples taken, embedded, sliced, and then stained with HE.Age, sex, tumor differentiation, depth of tumor invasion, clinicopathologic stage, lymph node metastasis and other clinicopathological data were analyzed.According to the diagnostic results of HE staining sections, the typical wax blocks were selected and the expression of PES1 and EGFR in gastric cancer and adjacent tissues was detected by using the two-step immunohistochemical method of S-P.The expression result is interpreted according to the prescribed standard, the data is sorted out, and the data is input into the SPSS17.0 statistical software for statistical analysis and processing.Results the positive expression rate of 1PES1 in 60 cases of gastric cancer was significantly higher than that in adjacent tissues (3.33%), and the difference was statistically significant. The positive expression rate of 1PES1 in gastric cancer tissues was significantly higher than that in adjacent tissues of gastric cancer (P 0.052.The difference was statistically significant.There was no correlation between the expression of PES1 and EGFR in gastric cancer tissues. The high expression of PES1 and EGFR in gastric cancer tissues was closely related to the depth of invasion of the tumor and the clinicopathologic stage (P0.05).The positive rate in patients with invasion of serous layer (41.86) was significantly higher than that in patients without serous layer (5.88%), and the positive rate in patients with stage 鈪，

本文編號(hào)：1757834