本文選題：RNAscope + 免疫組化　； 參考：《重慶醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:雌激素受體α(Estrogen receptorα,ERα)在乳腺癌的發(fā)生發(fā)展中起重要作用,臨床上用于判斷乳腺癌組織ERα表達(dá)的主要方法是免疫組化(IHC),但是IHC結(jié)果受諸多因素的影響。本文應(yīng)用RNAscope技術(shù)來檢測乳腺癌組織ERα的表達(dá),并將RNAscope的結(jié)果與IHC的結(jié)果進(jìn)行對比分析,旨在進(jìn)一步探討RNAscope技術(shù)的臨床應(yīng)用價值。方法:收集人乳腺癌組織標(biāo)本(72例,47例ERα陽性以及25例ERα陰性),將收集到的標(biāo)本分成三份,一份用來提取RNA,進(jìn)行RT-q PCR,一份用來進(jìn)行RNAscope原位雜交和IHC,一份備用;然后構(gòu)建標(biāo)準(zhǔn)曲線,進(jìn)行絕對定量RT-q PCR,并將絕對定量RT-q PCR以及RNAscope的結(jié)果與IHC進(jìn)行對比分析。結(jié)果:ERα陽性乳腺癌組織標(biāo)本的RT-qPCR結(jié)果和RNAscope結(jié)果顯著高于ERα陰性乳腺癌組織標(biāo)本,RT-q PCR的結(jié)果與IHC結(jié)果的一致性并不明顯,RNAscope的結(jié)果與IHC結(jié)果的一致性較好,在對不一致的組織標(biāo)本分析中我們發(fā)現(xiàn)有3例IHC判斷為ERα陰性而RNAscope判斷為陽性的標(biāo)本最后應(yīng)該診斷為陽性,有2例IHC判斷為ERα陽性RNAscope判斷為陰性的標(biāo)本最后應(yīng)該診斷為陰性,有1例IHC判斷為ERα陰性RNAscope判斷為陽性的標(biāo)本最后應(yīng)該診斷為陰性。結(jié)論:1.RNAscope結(jié)果與IHC結(jié)果具有較好的一致性,RT-q PCR結(jié)果與IHC結(jié)果一致性不明顯;且RNAscope操作較RT-q PCR操作簡便,還具有可視化,與RT-q PCR相比,具有明顯優(yōu)勢。2.為了提高ERα的診斷,RNAscope技術(shù)可以作為IHC的一種補(bǔ)充實(shí)驗(yàn)技術(shù)。
[Abstract]:Objective: estrogen receptor 偽 strogen receptor 偽 er 偽 plays an important role in the occurrence and development of breast cancer. The main method for judging the expression of ER 偽 in breast cancer is immunohistochemistry, but the results of IHC are affected by many factors.In this paper, the expression of ER 偽 in breast cancer tissue was detected by RNAscope technique, and the results of RNAscope and IHC were compared and analyzed in order to further explore the clinical application value of RNAscope technique.Methods: a total of 72 human breast cancer specimens were collected from 47 cases of ER 偽 positive and 25 cases of ER 偽 negative. The collected specimens were divided into three parts, one for RT-q, one for RNAscope in situ hybridization and one for RNAscope.Then the standard curve was constructed, and the absolute quantitative RT-q PCR and RNAscope results were compared with IHC.Results the results of RT-qPCR and RNAscope were significantly higher in the breast cancer tissue samples with ER 偽 positive than those in the samples with ER 偽 negative breast cancer. The results of RT-q PCR and IHC were not in good agreement with the results of IHC.In the analysis of inconsistent tissue specimens, we found that three specimens with ER 偽 negative for IHC and positive for RNAscope should be diagnosed as positive in the end, and 2 samples with ER 偽 positive RNAscope negative in IHC should be diagnosed as negative in the end.In one case, ER 偽 negative RNAscope positive specimen diagnosed by IHC should be diagnosed as negative in the end.Conclusion: 1. The results of RNAscope and IHC have good consistency. The results of RT-q PCR and IHC are not consistent, and the operation of RNAscope is simpler and more visual than that of RT-q PCR. Compared with RT-q PCR, it has obvious advantage. 2.In order to improve the diagnostic ability of ER 偽, it can be used as a supplementary experimental technique for IHC.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R737.9

【相似文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 韓笑;中腦邊緣皮質(zhì)多巴胺系統(tǒng)對獎賞效應(yīng)的精確調(diào)控與外源性大麻正性強(qiáng)化作用及其機(jī)制研究[D];中國人民解放軍軍事醫(yī)學(xué)科學(xué)院;2017年

相關(guān)碩士學(xué)位論文 前1條

1 喻修為;RNAscope技術(shù)與IHC在判斷乳腺癌組織ERα表達(dá)的比較研究[D];重慶醫(yī)科大學(xué);2017年

，

本文編號：1735467