本文選題：肺腺癌　切入點(diǎn)：葡萄糖轉(zhuǎn)運(yùn)蛋白(GLUT)　出處：《暨南大學(xué)學(xué)報(bào)(自然科學(xué)與醫(yī)學(xué)版)》2017年06期

【摘要】：目的:檢測(cè)果糖代謝相關(guān)蛋白葡萄糖轉(zhuǎn)運(yùn)蛋白5(GLUT5)和己酮糖激酶(KHK)在肺腺癌中的表達(dá)及生物學(xué)功能.方法:利用http://kmplot.com/analysis/上的數(shù)據(jù)分析GLUT5和KHK的mRNA表達(dá)水平對(duì)肺腺癌患者生存預(yù)后的影響;免疫組織化學(xué)方法檢測(cè)GLUT5和KHK蛋白在肺腺癌及相應(yīng)癌旁組織中的表達(dá)水平;SPSS統(tǒng)計(jì)分析GLUT5和KHK表達(dá)水平與肺腺癌患者年齡、性別、腫瘤大小、淋巴結(jié)轉(zhuǎn)移、遠(yuǎn)處轉(zhuǎn)移、臨床分期、病理分級(jí)等臨床病理指標(biāo)之間的關(guān)系,以及對(duì)患者生存預(yù)后的影響;MTT和克隆形成實(shí)驗(yàn)檢測(cè)無葡萄糖條件下果糖對(duì)肺腺癌細(xì)胞增殖和克隆形成的影響,以及沉默GLUT5和KHK對(duì)細(xì)胞生物學(xué)行為的影響.結(jié)果:GLUT5和KHK mRNA表達(dá)水平較高的肺腺癌患者,其總生存和無進(jìn)展生存均較差;GLUT5和KHK在肺腺癌組織中的蛋白表達(dá)水平均高于癌旁組織(P0.01);GLUT5高表達(dá)與腫瘤大小、淋巴結(jié)轉(zhuǎn)移和病理分化較差有關(guān)(P0.05);KHK高表達(dá)與腫瘤大小、臨床分期和病理分化較差有關(guān)(P0.05);而且GLUT5和KHK高表達(dá)的患者預(yù)后較差(P0.05).果糖可促進(jìn)肺腺癌細(xì)胞的增殖和克隆形成,沉默GLUT5和KHK后,細(xì)胞利用果糖的能力顯著下降.結(jié)論:果糖可以促進(jìn)肺腺癌細(xì)胞的增殖和克隆形成,果糖代謝相關(guān)蛋白GLUT5和KHK在肺腺癌組織中高表達(dá),且與肺腺癌的惡性進(jìn)展密切相關(guān),GLUT5和KHK可作為治療肺腺癌潛在臨床靶點(diǎn).
[Abstract]:Aim: to detect the expression and biological function of fructose metabolism-associated protein glucose transporter (GLUT5) and hexokokinase (KHK) in lung adenocarcinoma.Methods: http://kmplot.com/analysis/ data were used to analyze the effect of mRNA expression of GLUT5 and KHK on survival and prognosis of patients with lung adenocarcinoma.Expression of GLUT5 and KHK protein in Lung Adenocarcinoma and its adjacent tissues by immunohistochemical method GLUT5 and KHK expression levels and age, sex, tumor size, lymph node metastasis, distant metastasis, clinical stage were analyzed.The relationship between pathological grading and clinicopathological parameters, and the influence of MTT and Clone formation on the Survival and prognosis of patients the effects of fructose on the Proliferation and Clone formation of Lung Adenocarcinoma cells under Glucose-free condition,And the effect of silencing GLUT5 and KHK on cell biological behavior.Lymph node metastasis and poor pathological differentiation were related to the high expression of P0.05KHK and the poor clinical stage and pathological differentiation, and the prognosis of the patients with high expression of GLUT5 and KHK was poor.Fructose could promote the proliferation and clone formation of lung adenocarcinoma cells. After silencing GLUT5 and KHK, the ability of fructose utilization was significantly decreased.Conclusion: fructose can promote the proliferation and clone formation of lung adenocarcinoma cells. Fructose metabolism-related proteins GLUT5 and KHK are highly expressed in lung adenocarcinoma tissues, and GLUT5 and KHK are closely related to the malignant progression of lung adenocarcinoma. GLUT5 and KHK may be potential clinical targets for the treatment of lung adenocarcinoma.
【作者單位】： 河南中醫(yī)藥大學(xué)基礎(chǔ)醫(yī)學(xué)院實(shí)驗(yàn)教學(xué)中心;
【基金】：河南省高等學(xué)校重點(diǎn)科研項(xiàng)目(16A310021)
【分類號(hào)】：R734.2

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