本文選題：肥胖　切入點(diǎn)：H.suis　出處：《青島大學(xué)》2017年碩士論文

【摘要】：目的通過對(duì)Helicobacter suis(H.suis)感染肥胖小鼠的研究,探討高脂飲食誘導(dǎo)的肥胖對(duì)H.suis感染后小鼠胃黏膜相關(guān)淋巴樣組織(mucosa associated lymphoid tissue,MALT)形成的影響及其可能的機(jī)制,進(jìn)而有望為肥胖的促瘤機(jī)制提供新的研究方向。方法1.H.suis感染小鼠模型的建立:將40只雌性C57BL/6小鼠隨機(jī)分為模型組和對(duì)照組,模型組每只小鼠給予0.5 m L經(jīng)PCR檢測(cè)確定H.suis存在的豬胃黏膜勻漿液灌胃,對(duì)照組則給予等量磷酸鹽緩沖液(phosphate buffer saline,PBS)灌胃。分別于灌胃1個(gè)月及3個(gè)月后處死兩組中的半數(shù)小鼠,取其胃黏膜組織,采用PCR方法檢測(cè)H.suis在小鼠胃內(nèi)的定植情況,HE染色方法檢測(cè)胃黏膜淋巴濾泡形成的情況。2.肥胖對(duì)H.suis感染后胃MALT形成及相關(guān)炎癥因子表達(dá)的影響:建模成功后,新購(gòu)40只雌性C57BL/6小鼠隨機(jī)分為4組,每組10只,即正常對(duì)照組(NC組)、高脂飲食組(HFD組)、H.suis感染組(HS組)和H.suis感染+高脂飲食組(HS+HFD組),將已經(jīng)感染H.suis小鼠的胃黏膜勻漿液(0.2ml/只)灌胃于HS組與HS+HFD組。NC組與HS組持續(xù)給予普通飲食,HFD組與HS+HFD組自感染后第3周開始給予高脂飲食;差異性飲食24周后,處死各組小鼠取其胃組織,采用HE染色方法檢測(cè)各組小鼠胃黏膜淋巴濾泡的數(shù)量與大小;采用Real-time PCR方法檢測(cè)各組小鼠胃黏膜瘦素(leptin)及其受體Ob-R、炎癥因子IFN-γ與趨化因子CXCL13、CCL2的表達(dá)量。結(jié)果1.灌胃所用豬胃黏膜勻漿液中存在H.suis;H.suis可以在小鼠胃內(nèi)定植,1個(gè)月及3個(gè)月后均檢測(cè)到該菌;H.suis感染可誘導(dǎo)小鼠胃黏膜濾泡的形成,并且感染3個(gè)月較感染1個(gè)月的淋巴濾泡明顯增大。2.與HS組相比,HS+HFD組小鼠胃黏膜淋巴濾泡的數(shù)量與大小明顯增加,胃黏膜leptin及其受體Ob-R,IFN-γ、CXCL13、CCL2的m RNA表達(dá)量亦明顯增加。結(jié)論1.成功建立了穩(wěn)定可靠的H.suis感染小鼠模型;2.H.suis可以誘導(dǎo)出胃黏膜淋巴濾泡的形成;3.高脂飲食誘導(dǎo)的肥胖可能促進(jìn)了leptin→IFNγ→CXCL13鏈的表達(dá),進(jìn)而促進(jìn)H.suis感染后胃黏膜相關(guān)淋巴組織的形成。
[Abstract]:Objective to investigate the effect of obesity induced by high fat diet on the formation of mucosa associated lymphoid tissue (malt) in mice infected with Helicobacter suis (H. suis) and its possible mechanism.It is expected to provide a new research direction for the mechanism of obesity.Methods: 40 female C57BL/6 mice were randomly divided into model group and control group. Each mouse in the model group was given 0.5 mL PCR assay to determine the presence of H.suis in the gastric mucosa homogenate.The control group was given the same amount of phosphate buffer solution (Phosphate buffer saline).After 1 month and 3 months of gastric perfusion, half of the mice in the two groups were killed, and their gastric mucosal tissues were taken. The colonization of H.suis in the stomach of mice was detected by PCR method. The formation of lymphatic follicles in gastric mucosa was detected by HE staining.Effects of obesity on gastric MALT formation and expression of related inflammatory factors after H.suis infection: after successful modeling, 40 newly purchased female C57BL/6 mice were randomly divided into 4 groups, 10 in each group.The normal control group (NC group), the high-fat diet group (HFD group) and the high-fat diet group (HS group) and the H.suis infected high-fat diet group (HS HFD group) were perfused with 0.2 ml / mouse gastric mucosal homogenate of infected H.suis mice. The gastric mucosa homogenate of the infected mice was given intragastric to HS group and HS HFD group. NC group and HS group were maintained in HS group and HS group.The high fat diet was given to the general diet group and HS HFD group from the 3rd week after infection.After 24 weeks of different diet, the gastric tissues of each group were killed, and the number and size of lymphatic follicles in gastric mucosa of each group were detected by HE staining.The expression of leptin and its receptor Ob-R-, IFN- 緯 and CXCL13 CCL2 in gastric mucosa of mice in each group were detected by Real-time PCR method.Result 1.H. suis could be colonized in the gastric mucosa homogenate of pigs. After 1 and 3 months, it was found that H. suis infection could induce the formation of gastric follicles in mice.The number of lymphatic follicles in 3 months of infection was significantly larger than that in 1 month of infection.Compared with HS group, the number and size of lymphoid follicles in HS HFD group were significantly increased, and the expression of m RNA in gastric mucosal leptin and its receptor Ob-R- IFN- 緯 -CXCL13CCL2 was also significantly increased.Conclusion 1.A stable and reliable mouse model of H.suis infection was successfully established. 2. H. suis could induce the formation of lymphatic follicles in gastric mucosa.Obesity induced by high fat diet may promote the expression of IFN 緯 CXCL13 chain in leptin, and then promote the formation of gastric mucosal associated lymphoid tissue after H.suis infection.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.2;R589.2

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相關(guān)期刊論文 前10條

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本文編號(hào)：1721006