本文選題：乳腺癌　切入點(diǎn)：瘦素　出處：《免疫學(xué)雜志》2017年08期

【摘要】：目的探討瘦素對(duì)小鼠乳腺癌調(diào)節(jié)性T細(xì)胞的影響及生物學(xué)意義。方法培養(yǎng)4T1乳腺癌細(xì)胞株并接種于Balb/c小鼠建立乳腺癌模型,將成瘤小鼠隨機(jī)分為對(duì)照組和實(shí)驗(yàn)組,分別用等量生理鹽水(normal saline,NS)和瘦素(leptin)處理,監(jiān)測(cè)小鼠腫瘤的生長(zhǎng);流式細(xì)胞技術(shù)檢測(cè)各組Balb/c荷瘤小鼠外周血、淋巴結(jié)、脾臟中調(diào)節(jié)性T淋巴細(xì)胞的數(shù)量及比例變化;ELISA檢測(cè)各組荷瘤小鼠血清TGF-β1、IL-10含量。結(jié)果與NS組比較,實(shí)驗(yàn)組腫瘤生長(zhǎng)速度隨瘦素質(zhì)量濃度的增加而加快;外周血和脾臟中調(diào)節(jié)性T細(xì)胞比例隨瘦素質(zhì)量濃度的增加而升高(P0.05),淋巴結(jié)中調(diào)節(jié)性T細(xì)胞比例變化不明顯(P0.05),血清中TGF-β1、IL-10的含量隨瘦素質(zhì)量濃度的增加而升高(P0.05)。結(jié)論瘦素通過(guò)上調(diào)乳腺腫瘤微環(huán)境中調(diào)節(jié)性T細(xì)胞的數(shù)量比例,分泌抑制因子,抑制機(jī)體腫瘤免疫,進(jìn)而促進(jìn)小鼠乳腺腫瘤的生長(zhǎng)。
[Abstract]:Objective to investigate the effect of leptin on regulatory T cells of mouse breast cancer and its biological significance.Methods 4T1 breast cancer cell lines were cultured and inoculated into Balb/c mice to establish breast cancer model. The tumor-bearing mice were randomly divided into two groups: control group and experimental group. The mice were treated with the same amount of normal saline and leptin to monitor the growth of tumor.The quantity and proportion of regulatory T lymphocytes in peripheral blood, lymph nodes and spleen of Balb/c bearing mice were detected by flow cytometry.Results compared with NS group, the tumor growth rate of the experimental group was accelerated with the increase of leptin concentration.The proportion of regulatory T cells in peripheral blood and spleen increased with the increase of leptin concentration, while the proportion of regulatory T cells in lymph nodes did not change significantly (P 0.05). The content of TGF- 尾 1 and IL-10 in serum increased with the increase of leptin concentration.Conclusion leptin inhibits tumor immunity by upregulating the proportion of regulatory T cells in breast tumor microenvironment, secreting inhibitory factor, and then promoting the growth of breast tumor in mice.
【作者單位】： 桂林醫(yī)學(xué)院第二附屬醫(yī)院檢驗(yàn)科;廣西腫瘤免疫與微環(huán)境調(diào)控重點(diǎn)實(shí)驗(yàn)室;桂林醫(yī)學(xué)院免疫學(xué)教研室;
【基金】：國(guó)家自然科學(xué)基金(81260317)
【分類(lèi)號(hào)】：R737.9

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 侯子正;王金龍;黃華漳;李漪;;聯(lián)合應(yīng)用局部加溫與X線治療小鼠乳腺癌[J];天津醫(yī)藥腫瘤學(xué)附刊;1983年04期

2 于學(xué)寬,郭小紅,趙剛,曾嘉,沈偉,楊海賢,白景文;小鼠乳腺癌化療后殘留癌細(xì)胞的形態(tài)學(xué)研究[J];天津醫(yī)藥;2001年10期

3 于學(xué)寬,郭小紅,趙剛,曾嘉,沈偉,楊海賢,白景文;放療抵抗性小鼠乳腺癌細(xì)胞的形態(tài)觀察[J];天津醫(yī)藥;2002年03期

4 楊海賢,白景文,趙剛,沈偉,于學(xué)寬,郭小紅,曾嘉;小鼠乳腺癌化療放療后頓抑癌細(xì)胞超微結(jié)構(gòu)研究[J];中國(guó)腫瘤臨床;2002年06期

5 羅利群，張友會(huì);γ－干擾素促進(jìn)小鼠乳腺癌的轉(zhuǎn)移[J];中華腫瘤雜志;1994年04期

6 孫慧,牛瑞芳;基因表達(dá)譜芯片在小鼠乳腺癌組織中基因差異性表達(dá)[J];天津醫(yī)科大學(xué)學(xué)報(bào);2002年04期

7 陳信義,高志捷,王玉芝;薯蕷皂苷抗移植性小鼠乳腺癌作用的研究[J];中國(guó)中醫(yī)藥信息雜志;2005年05期

8 賈喜花;唐漢鈞;趙連臣;耿淑美;;乳寧Ⅱ號(hào)對(duì)小鼠乳腺癌抑瘤、抗轉(zhuǎn)移作用的實(shí)驗(yàn)研究[J];臨床誤診誤治;2006年06期

9 鮑濱;楊菁;黨麗捷;宋存先;;紫杉醇亞微球制備及治療小鼠乳腺癌的實(shí)驗(yàn)研究[J];生物醫(yī)學(xué)工程與臨床;2007年03期

10 李維才;周曉麗;王有Z，

本文編號(hào)：1702499