本文選題：微小RNA　切入點(diǎn)：胃癌　出處：《華中科技大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年04期

【摘要】：目的探討miR-551b在人胃癌組織中的表達(dá)及對(duì)胃癌細(xì)胞凋亡的影響。方法采用Real-time PCR檢測(cè)胃癌組織、正常胃組織miR-551b表達(dá)水平,分別將miRNA無義序列、miR-551bmimics、miR-551binhibitors轉(zhuǎn)染至人胃癌細(xì)胞株SGC-7901,Real-time PCR檢測(cè)各組細(xì)胞miR-551b表達(dá),MTT法檢測(cè)各組腫瘤細(xì)胞增殖活性,Transwell法檢測(cè)各組細(xì)胞侵襲能力,流式細(xì)胞術(shù)檢測(cè)各組細(xì)胞凋亡情況,Hoechst33342熒光染色觀察各組細(xì)胞自噬、凋亡的發(fā)生,Western blot檢測(cè)各組細(xì)胞NF-κB、LC3Ⅱ、Beclin 1蛋白表達(dá)。結(jié)果 miR-551b在胃癌組織表達(dá)明顯下調(diào),胃癌組織miR-551b相對(duì)表達(dá)量(1.75±0.13)顯著低于正常胃組織(2.47±0.38)(P0.05)。與NC組和miR-551binhibitors組比較,miR-551bmimics組miR-551b表達(dá)水平顯著上升,細(xì)胞增殖率和侵襲率顯著降低,細(xì)胞凋亡率明顯上升,各指標(biāo)比較差異均具有統(tǒng)計(jì)學(xué)意義(均P0.05)。Hoechst33342熒光染色顯示miR-551bmimics組出現(xiàn)大量自噬泡,NC組可見部分自噬泡,而miR-551binhibitors組僅有少量自噬泡。Western blot檢測(cè)結(jié)果顯示,與NC組和miR-551binhibitors組比較,miR-551bmimics組NF-κB、LC3Ⅱ、Beclin 1蛋白表達(dá)水平顯著升高(均P0.05)。結(jié)論 miR-551b能夠抑制胃癌細(xì)胞的增殖和侵襲等細(xì)胞生物功能,其作用機(jī)制可能是誘導(dǎo)了胃癌細(xì)胞發(fā)生自噬性凋亡。
[Abstract]:Objective to investigate the expression of miR-551b in human gastric carcinoma and its effect on the apoptosis of gastric cancer cells. Methods Real-time PCR was used to detect the expression of miR-551b in gastric cancer and normal gastric tissues. MiRNA nonsense sequence was transfected into human gastric cancer cell line SGC-7901Real-time PCR. MiR-551b expression in each group was detected. The proliferation activity of tumor cells in each group was detected by miRNA method and the invasiveness of tumor cells in each group was detected by Transwell method. Apoptosis was detected by flow cytometry and autophagy was observed by Hoechst33342 fluorescence staining. Apoptosis was detected by Western blot. The expression of NF- 魏 BnLC3 鈪，

本文編號(hào)：1695142