本文選題：白細胞介素6　切入點：卵巢腫瘤　出處：《青島大學》2017年碩士論文

【摘要】：目的:探究細胞因子信號抑制因子3(Suppressor of cytokine signaling protein 3,SOCS3)與白細胞介素6(Interleukin 6,IL-6)在卵巢癌組織中的表達及其臨床意義,并通過將不同終濃度的IL-6作用于卵巢癌SKOV3細胞株,檢測其對SKOV3細胞增殖能力的影響及SKOV3細胞中SOCS1和SOCS3表達的情況。方法:(1)采用免疫組化方法檢測105例卵巢癌組織、40例卵巢良性腫瘤組織和30例卵巢正常組織中SOCS3和IL-6的表達,并使用相關的統(tǒng)計學方法分析兩者的表達與卵巢癌臨床病理參數(shù)的關系。(2)不同終濃度的IL-6對卵巢癌細胞SKOV3進行處理,行Cell Counting Kit-8(CCK-8)、逆轉錄聚合酶鏈反應(reverse transcription polymerase chain reaction,RT-PCR)、蛋白免疫印跡法(Western Blot)分別檢測其對SKOV3細胞增殖能力的影響及細胞中SOCS1和SOCS3 mRNA和蛋白的表達。結果:(1)卵巢癌組織中SOCS3的陽性表達率顯著高于良性腫瘤組織及正常卵巢組織(SOCS3:X2=57.672,P0.01),卵巢癌組織中IL-6的陽性表達率顯著高于良性腫瘤組織及正常卵巢組織(X2=44.950,P0.01);SOCS3的過度表達與組織學分級及腹水有關(P0.05),其中,高級別且合并腹水的患者卵巢癌組織中的SOCS3陽性率顯著高于低級別未合并無腹水的患者組織中的陽性率(P0.05);IL-6的過度表達與組織學分級、FIGO分期及腹水有關(P0.05),其中,晚期、高級別且合并腹水的患者的卵巢癌組織中的IL-6陽性率顯著高于早期、低級別未合并腹水的患者組織中的陽性率(P0.05);Spearman相關分析表明SOCS3與IL-6的表達之間呈顯著正相關(r=0.574,P0.05)。(2)不同終濃度IL-6處理SKOV3細胞后,SKOV3細胞的增殖水平具有濃度依賴性(F=3531.369,P0.01)。不同終濃度的IL-6作用于SKOV3細胞后,SKOV3細胞中SOCS1和SOCS3 mRNA和蛋白的相對表達量呈現(xiàn)濃度依賴性(F=18.832、261.112、6.096、58.922,P0.01)。結論:(1)SOCS3在卵巢癌組織中呈現(xiàn)高表達,且SOCS3的過度表達與組織學分級、及腹水有關,而與卵巢癌的組織類型、淋巴結是否轉移、FIGO分期及年齡因素無關,其中,高級別且合并腹水的卵巢癌患者較低級別未合并腹水的卵巢癌患者相比,其癌組織中SOCS3的表達具有更高的陽性率。說明SOCS3的表達可能與卵巢癌的發(fā)生和發(fā)展具有密切的關聯(lián)。IL-6在卵巢癌組織中呈現(xiàn)高表達,且IL-6的過度表達與組織學分級、FIGO分期及腹水有關,而與卵巢癌的組織類型、淋巴結是否轉移及年齡因素無關。同時,晚期、高級別且合并腹水的卵巢癌患者較早期、低級別未合并腹水的卵巢癌患者相比,其癌組織中IL-6的表達具有更高的陽性率。這說明IL-6的過表達可能參與卵巢癌的形成,并且可能與卵巢癌病情進展有關。(2)隨著IL-6濃度的升高,可抑制SKOV3細胞中SOCS1 m RNA及蛋白的表達,促進SOCS3 mRNA及蛋白的表達,這表明IL-6可能通過控制卵巢癌細胞中SOCS1及SOCS3表達的不同,調控卵巢癌細胞SKOV3的增殖。
[Abstract]:Objective: to investigate the expression and clinical significance of cytokine signal suppressor 3(Suppressor of cytokine signaling protein 3 (SOCS3) and interleukin 6(Interleukin 6 (IL 6) in ovarian cancer tissues, and to explore the clinical significance of the expression of cytokine signal suppressor 3(Suppressor of cytokine signaling protein 3 (SOCS3) and interleukin 6(Interleukin 6 (IL 6) in ovarian cancer SKOV3 cells. Methods the expression of SOCS3 and IL-6 was detected by immunohistochemical method in 40 cases of ovarian benign tumor and 30 cases of normal ovarian tissues, and the expression of SOCS3 and IL-6 in 10 5 cases of ovarian carcinoma and 30 cases of normal ovarian tissues were detected by immunohistochemistry. The relationship between the expression of SKOV3 and clinicopathologic parameters of ovarian cancer was analyzed by correlation statistical method. (2) the SKOV3 of ovarian cancer cells was treated with different final concentrations of IL-6. Cell Counting Kit-8 CCK-8, reverse transcription polymerase chain reaction- reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting were used to detect the effect of Cell Counting Kit-8 CCK-8 and reverse transcriptase polymerase chain reaction (RT-PCR) on the proliferation of SKOV3 cells and the expression of SOCS1, SOCS3 mRNA and protein respectively. The positive expression rate of IL-6 in ovarian cancer tissues was significantly higher than that in benign tumor tissues and normal ovarian tissues. The positive rate of IL-6 expression in ovarian cancer tissues was significantly higher than that in benign tumor tissues and normal ovarian tissues, and the overexpression of SOCS3 was related to histological grading and ascites. The positive rate of SOCS3 in high grade patients with ascites was significantly higher than that in low grade patients without ascites. The positive rate of IL-6 in ovarian cancer tissues of high grade patients with ascites was significantly higher than that in early stage. Spearman correlation analysis showed that there was a significant positive correlation between the expression of SOCS3 and IL-6 in patients without ascites. There was a significant positive correlation between the expression of SOCS3 and IL-6. (2) the proliferation level of SKOV3 cells treated with different final concentrations of IL-6 was different in a dose-dependent manner. The relative expression of SOCS1 and SOCS3 mRNA and protein in SKOV3 cells after treated with IL-6 at the final concentration was in a concentration dependent manner. Conclusion the expression of SOCS1, SOCS3 mRNA and protein in ovarian cancer tissues is high. The expression of SOCS1 and SOCS3 mRNA and protein in SKOV3 cells is higher than that in SKOV3 cells, and the expression of SOCS1 and SOCS3 mRNA and protein in SKOV3 cells is higher than that in SKOV3 cells in a dose-dependent manner. The overexpression of SOCS3 was related to histological grade and ascites, but not to the histological type of ovarian cancer, Figo stage of lymph node metastasis and age factors. Patients with high grade ovarian cancer with ascites compared with those with lower grade ovarian cancer without ascites. The positive rate of SOCS3 expression in human ovarian cancer tissues was higher. It suggested that the expression of SOCS3 might be closely related to the occurrence and development of ovarian cancer. IL-6 was highly expressed in ovarian cancer tissues. The overexpression of IL-6 was related to histological grade, Figo stage and ascites, but not to the histological type, lymph node metastasis and age factors of ovarian cancer. The positive rate of IL-6 expression in ovarian cancer tissues without ascites was higher than that in patients without ascites. This suggests that overexpression of IL-6 may be involved in the formation of ovarian cancer. And it may be related to the progression of ovarian cancer. (2) with the increase of IL-6 concentration, it can inhibit the expression of SOCS1 m RNA and protein, and promote the expression of SOCS3 mRNA and protein in SKOV3 cells. This suggests that IL-6 may control the different expression of SOCS1 and SOCS3 in ovarian cancer cells. To regulate the proliferation of ovarian cancer cell line SKOV3.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R737.31

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