本文選題：乳腺癌　切入點(diǎn)：多柔比星　出處：《中國藥學(xué)雜志》2017年04期

【摘要】：目的研究絲甘蛋白聚糖(serglycin)的表達(dá)高低是否會(huì)影響乳腺癌細(xì)胞系對多柔比星的敏感性。方法用瞬時(shí)干擾的方法敲低絲甘蛋白聚糖在MDA-MB-231中的表達(dá)水平,且用qRT-PCR,免疫熒光的方法驗(yàn)證敲低效率;用MTS法測定多柔比星在陰性對照(NC)組和Si-絲甘蛋白聚糖(Si-SG)組中的IC_(50)值,以及測定在0.018μmol·L-1多柔比星的作用下兩組的增殖曲線;用克隆形成的方法觀察在不同藥物壓力下兩組克隆形成數(shù)的差異。結(jié)果瞬時(shí)干擾的效率達(dá)到70%以上;MTS方法檢測Si-SG組的IC_(50)值以及藥物壓力下的增殖曲線均顯著低于NC組;且在不同藥物壓力下的克隆形成能力也顯著低于NC組。結(jié)論在MDA-MB-231中瞬時(shí)干擾絲甘蛋白聚糖能夠顯著增強(qiáng)MDA-MB-231對多柔比星的敏感性。
[Abstract]:Objective to study whether the expression level of serglycinin affects the sensitivity of breast cancer cell line to doxorubicin. The knock efficiency was verified by qRT-PCR and immunofluorescence, the ICS-50 value of doxorubicin in negative control group and Si-seroglycan group was determined by MTS method, and the proliferation curve of the two groups under the action of 0.018 渭 mol L-1 doxorubicin was determined. Results the instantaneous interference efficiency was more than 70% in Si-SG group and the proliferation curve under drug pressure was significantly lower than that in NC group. The clone formation ability under different drug pressure was also significantly lower than that in NC group. Conclusion transient interference silk glycosaminoglycan in MDA-MB-231 can significantly enhance the sensitivity of MDA-MB-231 to doxorubicin.
【作者單位】： 中山大學(xué)附屬腫瘤醫(yī)院華南腫瘤學(xué)國家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金青年科學(xué)基金(81302317) 廣東省科技計(jì)劃項(xiàng)目(2014B020212017,2014A020209024)
【分類號】：R737.9
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本文編號：1669789