本文選題：芬苯達(dá)唑　切入點(diǎn)：慢性髓系白血病　出處：《中國病理生理雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的:探討抗寄生蟲藥芬苯達(dá)唑?qū)β运柘蛋籽〖?xì)胞K562的作用及機(jī)制。方法:采用CCK-8法檢測芬苯達(dá)唑?qū)562和正常人外周血單個(gè)核細(xì)胞(PBMC)生長的影響;臺盼藍(lán)拒染實(shí)驗(yàn)檢測芬苯達(dá)唑?qū)562細(xì)胞活力的影響;瑞氏染色觀察芬苯達(dá)唑?qū)562細(xì)胞形態(tài)的影響;流式細(xì)胞術(shù)檢測芬苯達(dá)唑?qū)562細(xì)胞周期分布的影響;Western blot檢測芬苯達(dá)唑?qū)562細(xì)胞周期相關(guān)蛋白表達(dá)的影響;免疫熒光觀察芬苯達(dá)唑?qū)562細(xì)胞核的改變。結(jié)果:芬苯達(dá)唑能夠顯著抑制K562細(xì)胞的生長,而對PBMC生長無明顯影響;進(jìn)一步的研究發(fā)現(xiàn),芬苯達(dá)唑顯著抑制K562細(xì)胞增殖并誘導(dǎo)細(xì)胞發(fā)生G_2/M期阻滯;芬苯達(dá)唑處理K562細(xì)胞后,細(xì)胞分裂周期蛋白25C(Cdc25C)磷酸化、周期素依賴性激酶1(Cdk1)-Tyr15去磷酸化以及cyclin B1磷酸化增加;免疫熒光結(jié)果證實(shí)芬苯達(dá)唑誘導(dǎo)K562多核細(xì)胞增多(P0.01),發(fā)生有絲分裂災(zāi)難。結(jié)論:芬苯達(dá)唑通過調(diào)控周期相關(guān)蛋白誘導(dǎo)K562細(xì)胞發(fā)生G_2/M期阻滯。
[Abstract]:Objective: to investigate the effect and mechanism of fenbendazole on the growth of K562 cells. Methods: the effects of fenbendazole on the growth of K562 and normal peripheral blood mononuclear cells (PBMCs) were detected by CCK-8 assay. The effect of fenbendazole on the viability of K562 cells was detected by trypan blue exclusion assay, and the effect of fenbendazole on the morphology of K562 cells was observed by Rayleigh staining. The effect of fenbendazole on cell cycle distribution of K562 cells was detected by flow cytometry. The effect of fenbendazole on the expression of cell cycle related proteins in K562 cells was detected by Western blot. Results: fenbendazole could significantly inhibit the growth of K562 cells, but had no effect on the growth of PBMC. Fenbendazole significantly inhibited K562 cell proliferation and induced GV / M arrest, and after treatment with fenbendazole, cell mitotic cyclin 25 cdc25C) phosphorylation, cyclin dependent kinase 1 (CDK 1) -Tyr15 dephosphorylation and cyclin B1 phosphorylation increased. The results of immunofluorescence showed that fenbendazole induced K562 cells to increase P0.01G and induce mitotic disaster. Conclusion: Fenbendazole induces G 2 / M arrest of K562 cells by regulating cycle related proteins.
【作者單位】： 溫州醫(yī)科大學(xué)檢生學(xué)院;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(No.81400125;No.81372826) 教育部博士點(diǎn)新教師類資助項(xiàng)目(No.20133321120003) 浙江省自然科學(xué)基金資助項(xiàng)目(No.LQ13H080002)
【分類號】：R733.72
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本文編號：1633316