本文選題：結(jié)直腸腺瘤　切入點：結(jié)直腸癌　出處：《北京協(xié)和醫(yī)學(xué)院》2015年博士論文　論文類型：學(xué)位論文

【摘要】：目的：研究兒茶酚氧位甲基轉(zhuǎn)移酶(COMT)在結(jié)直腸腺瘤及癌組織中的動態(tài)表達(dá)情況及其與結(jié)直腸癌(CRC)臨床病理性質(zhì)的相關(guān)性。方法：留取2013年1月至11月診治的18例結(jié)直腸腺瘤病例的新鮮腺瘤組織及瘤旁正常黏膜,提取RNA,進(jìn)行逆轉(zhuǎn)錄定量PCR。留取2013年1月至3月診治的49例結(jié)直腸腺瘤病例的新鮮腺瘤組織及瘤旁正常黏膜組織,制成切片,進(jìn)行免疫組化染色。留取2014年7月至8月診治的15例CRC病例的新鮮癌組織及癌旁正常黏膜組織,提取RNA,進(jìn)行逆轉(zhuǎn)錄定量PCR。篩選2012年6月至9月診治的42例CRC病例入組,將凍存的癌組織及癌旁正常黏膜制成切片,進(jìn)行免疫組化染色(其中1例同時進(jìn)行逆轉(zhuǎn)錄定量PCR)。篩選2007至2009年診治的81例CRC病例入組,查找病理科存放的相應(yīng)組織蠟塊并制片,進(jìn)行免疫組化染色。得到數(shù)據(jù)使用SPSS 13.0進(jìn)行配對t檢驗或獨(dú)立t檢驗進(jìn)行統(tǒng)計學(xué)分析。結(jié)果：18例進(jìn)行逆轉(zhuǎn)錄定量PCR的結(jié)直腸腺瘤病例中,編碼COMT的nRNA在腺瘤組織中的相對表達(dá)量明顯高于瘤旁正常對照組織,CI 95%,P=0.011。49例進(jìn)行免疫組化染色評分的結(jié)直腸腺瘤病例中,COMT蛋白在腺瘤組織中的表達(dá)明顯高于瘤旁正常對照組織,CI 95%,P=0.000。16例進(jìn)行逆轉(zhuǎn)錄定量PCR的CRC病例中,編碼COMT的mRNA在癌組織中的相對表達(dá)量明顯高于癌旁正常對照組織,CI95%,P=0.007。42例進(jìn)行免疫組化染色評分的CRC病例中,COMT蛋白在癌組織中的表達(dá)明顯高于癌旁正常對照組織,CI 95%,P=0.000。進(jìn)行免疫組化的81例CRC病例中,COMT蛋白在早期癌組織中的表達(dá)明顯高于進(jìn)展期癌組織,對于T/N/TNM, CI 95%, P=0.018/P=0.000/P=0.000,而早期癌組織中COMT蛋白的表達(dá)與腺瘤組織未發(fā)現(xiàn)明顯差異,對于T/N/TNM, CI 95%, P=0.933/P=0.527/P=0.227;另外,COMT蛋白在高、中分化癌組織中的表達(dá)明顯高于低分化癌組織,CI 95%,P=0.005,而高/中分化癌組織中COMT蛋白的表達(dá)與腺瘤組織未發(fā)現(xiàn)明顯差異,CI 95%,P=0.153。結(jié)論：COMT與結(jié)直腸腺瘤及癌的發(fā)生存在相關(guān)性,與CRC進(jìn)展過程及去分化過程存在相關(guān)性,具有作為CRC診斷、分期、分化程度的參考指標(biāo)的潛力。
[Abstract]:Objective: to study the dynamic expression of catechol-oxomethyltransferase (COMT) in colorectal adenoma and carcinoma and its correlation with clinicopathological properties of colorectal carcinoma. Methods: 18 patients with colorectal carcinoma from January 2013 to November were enrolled in this study. The fresh adenoma tissue and adjacent normal mucosa of colorectal adenoma. RNA was extracted from 49 cases of colorectal adenoma treated from January 2013 to March. The fresh adenoma tissue and normal mucosa adjacent to the tumor were collected and made into sections. From July 2014 to August, 15 cases of CRC were collected from the fresh cancer tissue and normal mucosa adjacent to the tumor, and then the RNA was extracted, and then reverse transcription quantitative PCR was performed. 42 cases of CRC from June 2012 to August were selected into the group. Frozen cancer tissue and normal mucosa adjacent to carcinoma were made into sections, and immunohistochemical staining was performed in one case. 81 cases of CRC diagnosed and treated from 2007 to 2009 were selected for examination and examination of corresponding tissue wax pieces stored in pathology department. SPSS 13.0 was used for paired t test or independent t test for statistical analysis. Results in 18 cases of colorectal adenoma with reverse transcription quantitative PCR, The relative expression of nRNA encoding COMT in adenoma tissue was significantly higher than that in adjacent normal control group (P0.011.49 cases with colorectal adenoma by immunohistochemical staining). In the normal control tissue, CIC95 / P was 0.000.16 cases of CRC with reverse transcription quantitative PCR. The relative expression of mRNA encoding COMT in cancer tissue was significantly higher than that in adjacent normal control group (P0.007.42 CRC cases), which was significantly higher than that in adjacent normal control group (P0.007.42) (P = 0.000). The expression of CRC protein in the early stage of cancer was significantly higher than that in the advanced stage of cancer, which was detected by immunohistochemistry in 81 cases of CRC. For T / N / TNM, CI95, P0. 018 / P0. 000 / P0. 000, there was no significant difference between the expression of COMT protein in early cancer tissue and adenoma tissue. For T / N / TNM, CI95, P0. 933 / P0. 527 / P0. 227; in addition, COMT protein was high. The expression of COMT protein in moderately differentiated carcinoma tissues was significantly higher than that in poorly differentiated carcinoma tissues. However, there was no significant difference between the expression of COMT protein and adenoma tissues. Conclusion there is a correlation between the expression of COMT protein and colorectal adenoma and carcinogenesis. It has the potential to be used as a reference index for the diagnosis, staging and differentiation of CRC.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級別】：博士
【學(xué)位授予年份】：2015
【分類號】：R735.34

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相關(guān)期刊論文 前1條

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本文編號：1633301