本文選題：多發(fā)性骨髓瘤　切入點(diǎn)：Notch基因　出處：《中國實(shí)驗(yàn)血液學(xué)雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的:探討RNAi沉默Notch1基因?qū)Χ喟l(fā)性骨髓瘤細(xì)胞在NOD/SCID小鼠體內(nèi)致瘤性的影響。方法:使用shRNA方法靶向沉默骨髓瘤RPMI8226細(xì)胞Notch1基因,建立NOD/SCID小鼠多發(fā)性骨髓瘤模型,觀察Notch1基因沉默后骨髓瘤的成瘤速度、大小的變化;用ELISA法檢測(cè)荷瘤小鼠血清中IL-6和VEGF水平變化。結(jié)果:Notch1-shRNA沉默Notch1基因后腫瘤細(xì)胞成瘤速度明顯減慢,腫瘤體積縮小,與對(duì)照組比較差異顯著。實(shí)驗(yàn)組小鼠血清中IL-6和VEGF水平與對(duì)照組比較明顯降低(P0.05)。結(jié)論:靶向沉默Notch1基因可顯著抑制骨髓瘤細(xì)胞的致瘤能力,其機(jī)制可能與Notch1基因沉默后瘤細(xì)胞分泌IL-6和VEGF的能力降低有關(guān),Notch1基因沉默有可能成為治療多發(fā)性骨髓瘤的新策略。
[Abstract]:Objective: to investigate the effect of RNAi silencing Notch1 gene on the tumorigenicity of multiple myeloma cells in NOD/SCID mice. Methods: the NOD/SCID mouse model of multiple myeloma was established by targeting the Notch1 gene of RPMI8226 cells with shRNA method. The tumorigenesis speed and size of myeloma were observed after Notch1 gene silencing, and the changes of IL-6 and VEGF levels in serum of mice bearing tumor were detected by ELISA method. Results the tumorigenesis rate and tumor volume of tumor cells decreased after Notch1 gene silencing with Notch1 gene silencing. Compared with the control group, the serum levels of IL-6 and VEGF in the experimental group were significantly lower than those in the control group. Conclusion: the target silencing Notch1 gene can significantly inhibit the tumorigenicity of myeloma cells. The mechanism may be related to the decrease in the ability of tumor cells to secrete IL-6 and VEGF after Notch1 gene silencing, which may be a new strategy for the treatment of multiple myeloma.
【作者單位】： 廈門大學(xué)附屬中山醫(yī)院血液科;貴州醫(yī)科大學(xué)第三附屬醫(yī)院血液科;
【基金】：國家自然科學(xué)基金(81172246) 貴州醫(yī)科大學(xué)-省科技合作項(xiàng)目(黔科合LH[2016]7391)
【分類號(hào)】：R733.3

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