發(fā)布時間：2018-03-12 21:30

  本文選題：宣威肺腺癌　切入點：長鏈非編碼RNA　出處：《中國腫瘤生物治療雜志》2017年07期 　論文類型：期刊論文

【摘要】：目的:檢測非編碼RNA FENDRR長鏈在宣威肺腺癌XWLC-05株細胞與非小細胞肺癌(NSCLC)A549株細胞中的表達,及其對細胞增殖、遷移及侵襲的影響。方法:實時熒光定量PCR檢測FENDRR基因在XWLC-05與A549細胞中的表達;構建過表達p EX-3-FENDRR質粒并轉染XWLC-05細胞,慢病毒干擾LV3-FENDRR載體轉染A549細胞,實時熒光定量PCR檢測轉染后XWLC-05和A549細胞內FENDRR的表達;MTS法檢測細胞增殖;Transwell小室法檢測細胞遷移及侵襲能力的變化。結果:XWLC-05細胞中FENDRR基因表達明顯較A549細胞低,成功提高轉染p EX-3-FENDRR的XWLC-05細胞和降低轉染LV3-FENDRR質粒的A549細胞中FENDRR m RNA水平。與對照組相比,過表達FENDRR可明顯抑制XWLC-05細胞增殖[(1.23±0.13)vs(1.46±0.25),P0.05]并降低其遷移、侵襲能力(P0.05);干擾FENDRR表達,能夠促進A549細胞增殖[(0.85±0.02)vs(0.77±0.08),P0.05]、遷移與侵襲能力(P0.05)。結論:lnc RNA FENDRR表達下調與宣威肺腺癌的發(fā)生發(fā)展相關,其在腫瘤惡性化過程中抑制腫瘤細胞的增殖與轉移。
[Abstract]:Objective: to detect the expression of non-coding RNA FENDRR long chain in Xuanwei lung adenocarcinoma XWLC-05 cell line and non-small cell lung cancer cell line A549, and to investigate its effect on cell proliferation. Methods: Real-time fluorescence quantitative PCR was used to detect the expression of FENDRR gene in XWLC-05 and A549 cells, and overexpression of p EX-3-FENDRR plasmid was constructed and transfected into XWLC-05 cells, and lentivirus interfered with LV3-FENDRR vector to transfect A549 cells. Real-time fluorescence quantitative PCR was used to detect the expression of FENDRR in XWLC-05 and A549 cells after transfection. The cell proliferation and transwell chamber assay were used to detect the changes of cell migration and invasion ability. Results the expression of FENDRR gene was significantly lower in WXWLC-05 cells than in A549 cells. Compared with the control group, overexpression of FENDRR could significantly inhibit the proliferation of XWLC-05 cells [1.23 鹵0.13 vs 1.46 鹵0.25 p0.05] and decrease its migration and invasion ability, and interfere with the expression of FENDRR. It can promote the proliferation of A549 cell line [0.85 鹵0.02VSD 0.77 鹵0.08P05], and the ability of migration and invasion is P0.05.Conclusion the down-regulation of RNA FENDRR expression is related to the occurrence and development of Xuan Wei lung adenocarcinoma, which inhibits the proliferation and metastasis of tumor cells during the malignant process of tumor.
【作者單位】： 昆明醫(yī)科大學第一附屬醫(yī)院檢驗科;昆明醫(yī)科大學第二附屬醫(yī)院檢驗科;昆明醫(yī)科大學第一附屬醫(yī)院急診外科;
【基金】：國家自然科學基金資助項目(No.81460064,No.81460325) 云南省科技廳昆醫(yī)聯(lián)合專項應用基礎研究基金資助項目(No.2015FB047) 云南省領軍人才計劃資助項目(L-201202)~~
【分類號】：R734.2

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本文編號：1603366