本文選題：維吾爾族　切入點：霍奇金淋巴瘤　出處：《新疆醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:探索EB病毒核心抗原-1(EBV nuclear antigen 1,EBNA-1)、潛伏膜蛋白-1(Latent membrane protein-1,LMP-1)基因分型與維吾爾族霍奇金淋巴瘤(Hodgkin's lymphoma,HL)發(fā)病的相關(guān)性。方法:采用原位雜交技術(shù)檢測新疆腫瘤醫(yī)院50例維吾爾族霍奇金淋巴瘤(HL)及60例淋巴結(jié)反應(yīng)性增生(LRH)活檢組織中EBV編碼的小RNA 1(EBV-encoded small RNA 1,EBER1)以篩選EBV陽性淋巴瘤標(biāo)本,采用PCR結(jié)合DNA測序技術(shù)對EBV陽性淋巴瘤標(biāo)本進(jìn)行EBV 1/2、C/D、F/f分型,并檢測EBV陽性淋巴瘤標(biāo)本中EBNA-1、LMP-1編碼基因分型。結(jié)果:(1)50例HL病理標(biāo)本中共有27例檢測到EBER1陽性信號,陽性率為54.0%。60例LRH病理標(biāo)本中共有8例檢測到EBER1陽性信號,陽性率為13.3%。(2)EBV 1/2、C/D、F/f分型在HL與LRH組中分布無統(tǒng)計學(xué)差異(P0.05)。(3)兩組共檢測到3種亞型,包括V-val型,P-thr型以及P-ala型,3種EBNA-1亞型在HL與LRH組中分布無統(tǒng)計學(xué)差異(P=1.00);3種EBNA-1亞型在HL亞型分布無統(tǒng)計學(xué)差異(P=0.731)。(4)LMP-1包括4種亞型(China 1、China2、Med-、Med+)及重組病毒株型的分布在HL與LRH分布無顯著差異(P=0.256);LMP-1亞型在各亞型HL分布差異無統(tǒng)計學(xué)意義(P=0.854)。結(jié)論:(1)EB病毒陽性維吾爾HL患者中,EB病毒感染以1型、F型感染為主。(2)EBV 1/2、C/D、F/f分型及EBNA-1、LMP-1亞型在病例組及對照組中分布均無顯著性差異,表明EBV 1/2、C/D、F/f亞型及EBNA-1、LMP-1亞型可能不具有腫瘤特異性。(3)V-val是維吾爾族HL患者EBNA-1中最為常見亞型,也是維吾爾族LRH中最常見的類型。(4)China-1是維吾爾族HL患者LMP-1中最為常見亞型。
[Abstract]:Objective: to investigate the relationship between EBV core antigen -1EBV nuclear antigen 1EBNA-1, latent membrane protein-1LMP-1) and the pathogenesis of Hodgkin's lymphoma in Uygur nationality. Methods: in situ hybridization technique was used to detect the incidence of Hodgkinia lymphoma in 50 cases of tumor hospital in Xinjiang. Methods: to investigate the relationship between the gene typing of EBV core antigen -1EBV nuclear antigen 1, latent membrane protein-1 (LMP-1) and the pathogenesis of Hodgkinine lymphoma in Uygur nationality. EBV encoding small RNA 1 EBV-encoded small RNA 1 EBER1) was used to screen EBV positive lymphoma specimens from our own Hodgkin's lymphoma and 60 cases of lymphaden-reactive hyperplasia (LRH) biopsy tissues. PCR and DNA sequencing techniques were used to detect the EBV 1 / 2 C / DU F / f typing of EBV positive lymphoma samples, and EBNA-1 LMP-1 coding genotyping was detected in EBV positive lymphoma samples. Results the EBER1 positive signals were detected in 27 out of 50 HL specimens from 50 cases of HL. The positive rate was 54.0.The positive rate of EBER1 was detected in 8 out of 60 pathological specimens of LRH, and the positive rate was 13.32.The positive rate was 13.32.The positive rate was 13.32.There was no statistical difference between HL and LRH in the distribution of 1 / 2C / D / F / F / f typing and there was no significant difference between the two groups in the distribution of EBER1 subtypes. There was no significant difference in the distribution of three EBNA-1 subtypes between HL and LRH. There was no significant difference in the distribution of three EBNA-1 subtypes between HL and LRH. There was no significant difference in the distribution of three EBNA-1 subtypes in HL subtype P0. 731, P 0. 731. The LMP-1 including 4 subtypes, China 1, China 2 Med-Med) and the distribution of recombinant virus strains in HL and LRH. There was no significant difference in the distribution of LMP-1 subtypes in all subtypes of HL. Conclusion the infection of Epstein-Barr virus in Uygur HL patients with 1: 1 EB virus positive is mainly caused by type 1 serotype F infection and EBV 1 / 2% C / D / D / f typing and EBNA-1 LMP-1 subtype in the case group and the control group. There was no significant difference in middle distribution, The results showed that the subtypes of EBV 1 / 2 C / D / F / f and EBNA-1G LMP-1 may not be tumor-specific. The most common subtypes of EBNA-1 in Uygur patients with HL are the most common subtypes of EBNA-1, and the most common subtypes of LMP-1 in Uygur patients with HL.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R733.1

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