天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

當(dāng)前位置:主頁(yè) > 醫(yī)學(xué)論文 > 腫瘤論文 >

Matrigel三維細(xì)胞培養(yǎng)方法培養(yǎng)的原代乳腺癌細(xì)胞生長(zhǎng)情況及對(duì)阿霉素敏感性觀察

發(fā)布時(shí)間:2018-03-09 06:00

  本文選題:乳腺腫瘤 切入點(diǎn):乳腺癌 出處:《山東醫(yī)藥》2017年19期  論文類(lèi)型:期刊論文


【摘要】:目的觀察Matrigel三維細(xì)胞培養(yǎng)方法培養(yǎng)的原代乳腺癌細(xì)胞生長(zhǎng)情況及其對(duì)阿霉素的敏感性。方法分別采用二維單層培養(yǎng)法、Matrigel三維細(xì)胞培養(yǎng)方法培養(yǎng)原代乳腺癌細(xì)胞。在培養(yǎng)的第1、3、5、7天,采用Olympus Ⅸ70倒置顯微鏡觀察二維單層培養(yǎng)及三維培養(yǎng)條件下的原代乳腺癌細(xì)胞形態(tài),采用CellTiter-Glo~汶發(fā)光法觀察細(xì)胞生長(zhǎng)增殖情況及其對(duì)阿霉素的敏感性,繪制原代乳腺癌細(xì)胞生長(zhǎng)曲線及藥敏曲線。結(jié)果二維單層培養(yǎng)的原代乳腺癌細(xì)胞貼壁生長(zhǎng),癌細(xì)胞聚集成巢狀,呈多種形態(tài)。三維細(xì)胞培養(yǎng)的原代乳腺癌細(xì)胞聚集成緊密的多腫瘤細(xì)胞微球。二維單層培養(yǎng)第1、3、5、7天原代乳腺癌細(xì)胞的增殖倍數(shù)分別為1.00±0.00、1.39±0.29、2.08±0.25、3.26±0.41;三維培養(yǎng)第1、3、5、7天原代乳腺癌細(xì)胞的增殖倍數(shù)分別為1.00±0.00、1.25±0.08、1.79±0.10、1.58±0.02。隨阿霉素濃度升高,二維單層培養(yǎng)及三維培養(yǎng)下的原代乳腺癌細(xì)胞增殖抑制率增高。但三維細(xì)胞培養(yǎng)的原代乳腺癌細(xì)胞增殖抑制率均低于二維細(xì)胞培養(yǎng)。結(jié)論 Matrigel三維細(xì)胞培養(yǎng)的原代乳腺癌細(xì)胞可聚集成緊密的球形,細(xì)胞增殖速度變慢,對(duì)阿霉素的敏感性降低。Matrigel三維細(xì)胞培養(yǎng)原代乳腺癌細(xì)胞效果較好。
[Abstract]:Objective to observe the growth of primary breast cancer cells cultured by Matrigel three-dimensional cell culture method and its sensitivity to adrigel. Methods Primary breast cancer cells were cultured by two-dimensional monolayer culture method and Matrigel three-dimensional cell culture method respectively. After 7 days of culture, The morphology of primary breast cancer cells in 2-D monolayer culture and three-dimensional culture was observed by inverted microscope of Olympus IX 70. The cell proliferation and its sensitivity to doxorubicin were observed by CellTiter-Glo-Wen luminescence method. Results the primary breast cancer cells cultured in two dimensional monolayers adhered to the wall and the cancer cells gathered into nests. The primary breast cancer cells cultured in three dimensional cells were clustered into compact multi-tumor cell microspheres. The proliferation times of primary breast cancer cells were 1.00 鹵0.000.39 鹵0.290.39 鹵0.253.26 鹵0.41 on the 7th day of one-dimensional monolayer culture, respectively, and the primary mammary gland cells were primary mammary gland cells on the 1st 357th day of three-dimensional culture. The proliferation times of cancer cells were 1.00 鹵0.001. 25 鹵0. 08 鹵1. 79 鹵0. 10 and 1. 58 鹵0. 02 respectively. The inhibition rate of primary breast cancer cell proliferation in two-dimensional monolayer culture and three-dimensional culture was increased, but the inhibition rate of primary breast cancer cell proliferation in three-dimensional cell culture was lower than that in two-dimensional cell culture. Breast cancer cells can assemble into compact globes. The cell proliferation rate became slower and the sensitivity to adrigel decreased. Matrigel three-dimensional cell culture of primary breast cancer cells had a better effect.
【作者單位】: 天津醫(yī)科大學(xué)腫瘤醫(yī)院國(guó)家腫瘤臨床醫(yī)學(xué)研究中心;天津市"腫瘤防治"重點(diǎn)實(shí)驗(yàn)室;天津市惡性腫瘤臨床醫(yī)學(xué)研究中心;乳腺癌防治教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】:中國(guó)醫(yī)學(xué)基金會(huì)腫瘤預(yù)防與科研項(xiàng)目(314.2222)
【分類(lèi)號(hào)】:R737.9
,

本文編號(hào):1587253

資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/yixuelunwen/zlx/1587253.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶(hù)50787***提供,本站僅收錄摘要或目錄,作者需要?jiǎng)h除請(qǐng)E-mail郵箱bigeng88@qq.com