本文選題：乳腺腫瘤　切入點：乳腺癌　出處：《山東醫(yī)藥》2017年19期 　論文類型：期刊論文

【摘要】：目的觀察Matrigel三維細胞培養(yǎng)方法培養(yǎng)的原代乳腺癌細胞生長情況及其對阿霉素的敏感性。方法分別采用二維單層培養(yǎng)法、Matrigel三維細胞培養(yǎng)方法培養(yǎng)原代乳腺癌細胞。在培養(yǎng)的第1、3、5、7天,采用Olympus Ⅸ70倒置顯微鏡觀察二維單層培養(yǎng)及三維培養(yǎng)條件下的原代乳腺癌細胞形態(tài),采用CellTiter-Glo~汶發(fā)光法觀察細胞生長增殖情況及其對阿霉素的敏感性,繪制原代乳腺癌細胞生長曲線及藥敏曲線。結(jié)果二維單層培養(yǎng)的原代乳腺癌細胞貼壁生長,癌細胞聚集成巢狀,呈多種形態(tài)。三維細胞培養(yǎng)的原代乳腺癌細胞聚集成緊密的多腫瘤細胞微球。二維單層培養(yǎng)第1、3、5、7天原代乳腺癌細胞的增殖倍數(shù)分別為1.00±0.00、1.39±0.29、2.08±0.25、3.26±0.41;三維培養(yǎng)第1、3、5、7天原代乳腺癌細胞的增殖倍數(shù)分別為1.00±0.00、1.25±0.08、1.79±0.10、1.58±0.02。隨阿霉素濃度升高,二維單層培養(yǎng)及三維培養(yǎng)下的原代乳腺癌細胞增殖抑制率增高。但三維細胞培養(yǎng)的原代乳腺癌細胞增殖抑制率均低于二維細胞培養(yǎng)。結(jié)論 Matrigel三維細胞培養(yǎng)的原代乳腺癌細胞可聚集成緊密的球形,細胞增殖速度變慢,對阿霉素的敏感性降低。Matrigel三維細胞培養(yǎng)原代乳腺癌細胞效果較好。
[Abstract]:Objective to observe the growth of primary breast cancer cells cultured by Matrigel three-dimensional cell culture method and its sensitivity to adrigel. Methods Primary breast cancer cells were cultured by two-dimensional monolayer culture method and Matrigel three-dimensional cell culture method respectively. After 7 days of culture, The morphology of primary breast cancer cells in 2-D monolayer culture and three-dimensional culture was observed by inverted microscope of Olympus IX 70. The cell proliferation and its sensitivity to doxorubicin were observed by CellTiter-Glo-Wen luminescence method. Results the primary breast cancer cells cultured in two dimensional monolayers adhered to the wall and the cancer cells gathered into nests. The primary breast cancer cells cultured in three dimensional cells were clustered into compact multi-tumor cell microspheres. The proliferation times of primary breast cancer cells were 1.00 鹵0.000.39 鹵0.290.39 鹵0.253.26 鹵0.41 on the 7th day of one-dimensional monolayer culture, respectively, and the primary mammary gland cells were primary mammary gland cells on the 1st 357th day of three-dimensional culture. The proliferation times of cancer cells were 1.00 鹵0.001. 25 鹵0. 08 鹵1. 79 鹵0. 10 and 1. 58 鹵0. 02 respectively. The inhibition rate of primary breast cancer cell proliferation in two-dimensional monolayer culture and three-dimensional culture was increased, but the inhibition rate of primary breast cancer cell proliferation in three-dimensional cell culture was lower than that in two-dimensional cell culture. Breast cancer cells can assemble into compact globes. The cell proliferation rate became slower and the sensitivity to adrigel decreased. Matrigel three-dimensional cell culture of primary breast cancer cells had a better effect.
【作者單位】： 天津醫(yī)科大學腫瘤醫(yī)院國家腫瘤臨床醫(yī)學研究中心;天津市"腫瘤防治"重點實驗室;天津市惡性腫瘤臨床醫(yī)學研究中心;乳腺癌防治教育部重點實驗室;
【基金】：中國醫(yī)學基金會腫瘤預防與科研項目(314.2222)
【分類號】：R737.9
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本文編號：1587253