發(fā)布時間：2018-03-02 16:24

  本文選題：DEPDC1　切入點：甲狀腺乳頭狀癌　出處：《青島大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：背景:國內(nèi)外大量研究表明目前甲狀腺癌的發(fā)病率不斷上升,而在甲狀腺癌所有病理類型中乳頭狀甲狀腺癌占半數(shù)以上。雖然甲狀腺乳頭狀癌的致病因素目前仍不十分明確,但是幾乎所有的惡性腫瘤均有正常細胞周期調(diào)節(jié)的缺失。分析調(diào)控細胞周期的DEPDC1蛋白與甲狀腺乳頭狀癌及甲狀腺乳頭狀癌中最重要的分子標記物BRAF基因的關(guān)聯(lián)對甲狀腺乳頭狀癌的發(fā)病機制研究具有重要意義。目的:研究甲狀腺乳頭狀癌組織及瘤旁正常甲狀腺組織中DEPDC1的表達情況,研究BRAF基因野生型甲狀腺乳頭狀癌腫瘤組織中及BRAF基因突變型甲狀腺乳頭狀癌腫瘤組織中DEPDC1表達差異情況,分析DEPDC1與甲狀腺乳頭狀癌及BRAF基因突變及各臨床特征之間的關(guān)系。為研究DEPDC1是否可以作為甲狀腺乳頭狀癌的臨床診斷及治療靶點提供實驗依據(jù)。方法:采用以醫(yī)院臨床標本為基礎(chǔ)的對照研究,擬應(yīng)用分子流行病學(xué)的方法,利用已有的標本提取組織蛋白及制作病理切片。應(yīng)用免疫組化方法檢測甲狀腺乳頭狀癌腫瘤組織及瘤旁正常甲狀腺組織中DEPDC1表達部位。應(yīng)用免疫印跡法檢測甲狀腺乳頭狀癌腫瘤組織及瘤旁正常甲狀腺組織、BRAF基因野生型腫瘤組織及BRAF基因突變型腫瘤組織中、不同臨床特征分組甲狀腺乳頭狀癌腫瘤組織中DEPDC1表達差異。結(jié)果:免疫組化結(jié)果顯示DEPDC1在甲狀腺乳頭狀癌及瘤旁正常組織中表達。在5例腫瘤組織病理切片中共檢測到陰性表達1例,強陽性表達4例,2例瘤旁正常組織中均檢測到弱陽性表達。DEPDC1蛋白定位于細胞核及細胞質(zhì)中。免疫印跡結(jié)果顯示DEPDC1蛋白在甲狀腺乳頭狀癌腫瘤組織中表達情況為(59(14)62),免疫印跡結(jié)果顯示DEPDC1蛋白在瘤旁組織表達情況為(14(14)20)。免疫印跡結(jié)果顯示DEPDC1蛋白在甲狀腺乳頭狀癌組織中表達量為(1.60±0.82),明顯高于DEPDC1蛋白在瘤旁正常甲狀腺組織中表達量(0.58±0.19)其差異有統(tǒng)計學(xué)意義(P0.05)。DEPDC1蛋白在BRAF基因突變型腫瘤組織中表達量為(1.83±0.80),明顯高于在BRAF基因野生型腫瘤組織表達量(1.03±0.45)差異有統(tǒng)計學(xué)意義(P0.05)。男性患者及女性患者腫瘤組織中DEPDC1蛋白表達量分別為(1.41±0.71)、(1.64±0.83)該差異無統(tǒng)計學(xué)意義(P0.05)。年齡大于等于45歲及小于45歲患者腫瘤組織中DEPDC1蛋白表達量分別為(1.51±0.67)、(1.64±0.91)該差異無統(tǒng)計學(xué)意義(P0.05)。上述實驗結(jié)果顯示DEPDC1蛋白在甲狀腺乳頭狀癌組織及瘤旁正常組織中均可檢測到存在,且腫瘤組織中表達強度明顯高于瘤旁正常甲狀腺組織中表達強度,DEPDC1蛋白表達程度與甲狀腺乳頭狀癌的特異性分子標記物突變型BRAF基因呈正相關(guān),而DEPDC1蛋白的表達與性別、年齡等臨床特征無明顯關(guān)聯(lián)。結(jié)論:DEPDC1蛋白定位于甲狀腺乳頭狀癌細胞的細胞核及細胞質(zhì)中,DEPDC1的表達與甲狀腺乳頭狀癌及BRAF基因突變呈正相關(guān),與性別及年齡無相關(guān)。
[Abstract]:Background: a large number of domestic and foreign research shows that the incidence of thyroid cancer is rising, while the nipple in thyroid cancer all pathological types of thyroid cancer accounts for more than half. Although the causative factors of papillary thyroid carcinoma is not very clear, but the lack of almost all malignant tumors have normal cell cycle regulation is of great significance. The pathogenesis of related molecular markers of BRAF gene is the most important regulation of cell cycle and DEPDC1 protein in papillary thyroid carcinoma and thyroid papillary carcinoma of thyroid papillary carcinoma. Objective: To study the expression of DEPDC1 in papillary thyroid carcinoma and normal thyroid tissues adjacent to tumor in the study of wild type BRAF gene in papillary thyroid papillary carcinoma in tumor tissue and BRAF gene mutation between the expression of DEPDC1 type papillary thyroid carcinoma tissues, and DEPDC1 analysis The relationship between papillary thyroid carcinoma and BRAF gene mutation and the clinical features. To study whether DEPDC1 can be used in clinical diagnosis and treatment of papillary thyroid carcinoma and provide experimental basis. Methods: a comparative study on clinical samples of hospital based by the method of quasi applications of molecular epidemiology, and make pathological tissue protein extraction by using the existing specimens. The expression of DEPDC1 was detected by immunohistochemistry method in papillary thyroid cancer tissues and normal thyroid tissues adjacent to tumor site. By immunoblotting detection of thyroid papillary carcinoma tumor tissue and tumor adjacent normal thyroid tissue, tumor tissue of wild type BRAF gene and BRAF gene mutation in tumor tissues, DEPDC1 expression different clinical features of papillary thyroid carcinoma group. Results: immunohistochemistry results showed that DEPDC1 in papillary thyroid carcinoma Expression and tumor adjacent normal tissues. In 5 cases of pathological sections of tumor tissues were detected in 1 cases of negative expression, 4 cases were strongly positive, 2 cases of tumor adjacent normal tissues were detected by weak positive expression of.DEPDC1 protein was localized in the nucleus and cytoplasm. The results of Western blot showed that the expression of DEPDC1 protein in papillary thyroid carcinoma in case (59 (14) 62), the results of Western blot showed that DEPDC1 protein in tumor tissues for expression (14 (14) 20). The results of Western blot showed that the expression of DEPDC1 protein in papillary thyroid carcinoma was (1.60 + 0.82), significantly higher than that of DEPDC1 protein in tumor adjacent normal expression the amount of thyroid tissue (0.58 + 0.19) the difference was statistically significant (P0.05) expression of mutant.DEPDC1 protein in tumor tissue was in the BRAF gene (1.83 + 0.80), was significantly higher than that of wild type BRAF gene expression in tumor tissue (1.03 + 0.45) difference Statistical significance (P0.05). The expression of DEPDC1 protein were male patients and female patients in tumor tissues (1.41 + 0.71), (1.64 + 0.83) the difference was not statistically significant (P0.05). Older than 45 years and less than the amount of respectively the expression of DEPDC1 protein in tumor tissue of 45 (1.51 + 0.67 (1.64 + 0.91)), the difference was not statistically significant (P0.05). The experimental results showed that DEPDC1 protein could be detected in the presence of papillary thyroid carcinoma and tumor adjacent normal tissues, expression intensity was significantly higher than that of tumor adjacent normal thyroid tissues and tumor tissues, specific molecular markers for sudden the mutant BRAF gene positive expression of DEPDC1 protein and the degree of papillary thyroid carcinoma, and the expression of DEPDC1 protein was associated with gender, clinical features of age. Conclusion: the localization of DEPDC1 protein in thyroid papillary carcinoma cell and nucleus In the cytoplasm, the expression of DEPDC1 is positively correlated with the thyroid papillary carcinoma and the mutation of the BRAF gene, and is not related to the sex and age.

【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R736.1

【參考文獻】

相關(guān)期刊論文 前7條

1 茹曉婷;劉勤江;楊榮;;分化型甲狀腺癌NIS與TSHR研究進展[J];甘肅醫(yī)藥;2016年04期

2 譚慧心;董彥宏;關(guān)文輝;張鯤;徐健;;細胞周期調(diào)控與腫瘤發(fā)生和治療的關(guān)系研究[J];哈爾濱商業(yè)大學(xué)學(xué)報(自然科學(xué)版);2015年06期

3 任改艷;孫阿寧;張晶晶;鄧超;王崢濤;竇薇;;NF-κB在細胞凋亡中調(diào)節(jié)作用的研究進展[J];中國藥理學(xué)與毒理學(xué)雜志;2015年02期

4 何龍霞;糜燕;張春冬;張瑩;蔡偉;王義濤;卜友泉;朱江;;沉默DEPDC1基因表達對鼻咽癌細胞系HNE-1生長和細胞周期的影響[J];中國細胞生物學(xué)學(xué)報;2014年03期

5 李清懷;張曉娟;李筱雨;底旺;張霖雷;;促甲狀腺激素受體在分化型甲狀腺癌組織中的表達及意義[J];中華腫瘤雜志;2013年10期

6 金曉燕;權(quán)明明;楊林軍;楊晶金;王依滿;沈峰清;蔡軍波;梁勇;;Notch-2在甲狀腺乳頭狀癌中的表達[J];中國中西醫(yī)結(jié)合外科雜志;2012年06期

7 李建周;金勇君;劉欣;張凌云;;血清促甲狀腺素水平與甲狀腺癌發(fā)病的相關(guān)性[J];中華腫瘤雜志;2011年12期

，

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